Chicken coccidiosis vaccine suspending agent and fluid suspension therewith

A technology of chicken coccidiosis and suspending agent, which is applied in the field of suspending agent and suspension of chicken coccidiosis live oocyst vaccine, can solve the problems of slow dissolving speed of carboxymethyl cellulose and is not, and achieves the reduction of stoppage time. Water stress, reduced side effects, improved uniformity

Active Publication Date: 2012-10-17
齐鲁青大生物制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, carboxymethyl cellulose dissolves slowly in water and needs to be soaked in water for a long time to dissolve. For example, in the case of manual crushing, it takes more than ten hours to dissolve
Therefore, the cellulose ether is not an ideal suspending agent for chicken coccidiosis vaccine

Method used

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  • Chicken coccidiosis vaccine suspending agent and fluid suspension therewith
  • Chicken coccidiosis vaccine suspending agent and fluid suspension therewith
  • Chicken coccidiosis vaccine suspending agent and fluid suspension therewith

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1. get xanthan gum 3.6 grams, 25 milliliters of 95% ethanol aqueous solution mix as suspending agent, this suspending agent is poured into 6 kilograms of water and stir, and this suspending agent dissolves very soon. Containing 2 ml of sporulated oocyst fluid containing Eimeria acuminata, Eimeria giant, Eimeria tenella, and Eimeria poisonous (each ml contains 1 sporulated oocyst) 10,000), shake well, and divide into 2 portions of 1 ml each. Twenty 4-day-old chickens were divided into 2 groups of 10 each. Take 9 milliliters of the dissolved suspending agent solution of the invention and mix it with 1 part of coccidioides sporulated oocyst liquid, and inoculate 1 group of chickens with 1 milliliter per chicken (1000 sporulated oocysts per milliliter). ; The second group of chickens does not add suspending agent solution, mixes 9 milliliters of water with 1 part of chicken coccidia sporulation oocyst liquid, and inoculates 1 milliliter of each chicken (1000 spo...

Embodiment 2

[0032] Take xanthan gum at 3.6 grams per serving. Take the volume concentration as 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 55%, 65%, 75% %, 85%, 95%, and 100% ethanol aqueous solutions are 25 ml each. Mix one part of ethanol aqueous solution with one part of xanthan gum, shake well and pour into 6 kg of water and stir, the suspending agent dissolves quickly, and the results are shown in Table 2.

[0033] Table 2. Relationship between ethanol concentration and dissolution time

[0034]

[0035] Experiments have proved that with the increase of ethanol concentration, the dissolution rate is accelerated; especially when the concentration of ethanol aqueous solution is 45%~100%, the dissolution time is shorter, so the concentration of ethanol aqueous solution is preferably 45%~100%. When the concentration of ethanol aqueous solution is 75%, the antiseptic effect of the solution is the best, so 75% ethanol aqueous solution is preferred.

Embodiment 3

[0037] Take xanthan gum 3 grams, 6 grams, 9 grams, 12 grams, 15 grams, 18 grams, 21 grams, 24 grams, 27 grams, 30 grams, 33 grams, 36 grams, 39 grams, 42 grams, 45 grams, 48 ​​grams gram, 51 gram, 54 gram, 57 gram and 60 gram are mixed with 100 milliliters of 50% (volume ratio) ethanol aqueous solution respectively, after shaking well, pour into 20 kilograms of water and stir, and this suspending agent all dissolves within 2 minutes, The specific dissolution time is shown in Table 3. Experiments have shown that when the ratio of xanthan gum to ethanol aqueous solution is below 24 grams: 100 ml, the suspending agent dissolves faster in water, so 3-24 grams of xanthan gum: 100 ml ethanol aqueous solution is preferred.

[0038] Table 3 The relationship between the grams of xanthan gum and the dissolution time

[0039]

[0040] Take 250 grams of sodium carboxymethylcellulose (CMC), slowly add a small amount to 100 kilograms of water and stir, and the dissolution rate is very s...

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Abstract

The invention discloses a chicken coccidiosis vaccine suspension agent and suspension fluid therewith. The chicken coccidiosis vaccine suspension agent comprises xanthan gum and aqueous ethanol, wherein the volume concentration of the aqueous ethanol is greater than 38% and smaller then or equal to 100%, and a weight-to-volume ratio of xanthan gum to aqueous ethanol is from 3% to 60%. Xanthan gum is preferably selected fromfood-grade and pharmaceutical grade xanthan gum, and ethanol is preferably selected from food-grade and pharmaceutical grade ethanol. The invention further discloses suspension fluid prepared by using the suspending agent. The invention is advantaged in that: first, the suspension agent can dissolve in water rapidly; second, prepared chicken coccidiosis live vaccine oocysts can be kept in water by the prepared suspension fluid for 8 hour without sedimentation such that quantity uniformity of vaccine oocysts drunk by chickens can be improved; third, non-water-stopping chicken flock immunization can be carried out, and water-stopping stress response is reduced; fourth, water-stopping chicken flock immunization can further be carried out, and immunization time is shortened; and fifth, a side effect of the vaccine is reduced.

Description

technical field [0001] The present invention relates to a chicken coccidiosis vaccine suspending agent and suspension, in particular to a chicken coccidiosis live oocyst vaccine suspending agent and suspension used for drinking water immunity, the suspending agent can be quickly dissolved ; It can also slow down the sinking of vaccine oocysts for up to 8 hours, and enable chickens to be immunized without stopping water or immunizing without water, and can reduce the side effects of vaccines in both cases. Background technique [0002] Chicken coccidiosis is a kind of protozoan parasitic disease, by Eimeria tenaca, Eimeria pileosa, Eimeria giant, Eimeria poisonous, Eimeria placidum, Caused by one or more species of Eimeria brucei and Eimeria prematuria. They parasitize in various parts of the intestinal tract of chickens, causing intestinal inflammation, bleeding and death of chickens, causing huge economic losses to the chicken industry. [0003] For a long time, the preve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/36A61K47/10
Inventor 郝言芝王江青
Owner 齐鲁青大生物制药有限公司
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