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Bacillus subtilis and application thereof in preventing and treating plant leaf spot diseases

A Bacillus subtilis and plant leaf technology, applied in the field of microorganisms, can solve problems such as unsatisfactory leaf spot disease of Dendrobium officinale, and achieve the effects of good development and application prospects, easy industrial production, and easy preservation.

Active Publication Date: 2012-10-17
JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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Problems solved by technology

[0010] At present, there is no ideal biocontrol agent for the prevention and treatment of Dendrobium candidum leaf spot. If biological pesticides or as few pesticides as possible can be used to effectively control Dendrobium candidum leaf spot, pesticide residues will be reduced to a certain extent, and its food safety

Method used

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  • Bacillus subtilis and application thereof in preventing and treating plant leaf spot diseases
  • Bacillus subtilis and application thereof in preventing and treating plant leaf spot diseases
  • Bacillus subtilis and application thereof in preventing and treating plant leaf spot diseases

Examples

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Embodiment 1

[0028] Bacillus subtilis (Bacillus subtilis) JSNL1205 on Dendrobium candidum leaf spot pathogen Alternaria brassicae (Alternaria brassicae), Photinia leaf spot pathogen Botryotinia fuckeliana, strawberry leaf spot pathogen microsporum Plate bacteriostasis of Pestalotiopsis microspora.

[0029] Inoculate the Bacillus subtilis (Bacillus subtilis) JSNL1205 strain activated on the LB slope symmetrically on both sides of the PDA plate, and then inoculate the PDA plate culture of the pathogenic bacteria of Dendrobium candidum, Photinia fragrans and strawberry leaf spot with a hole puncher with a diameter of 5mm Take the same amount of fungus cake and inoculate it in the center of the plate. Cultivate at 27±1°C for 10 days to observe the antibacterial effect, and take pictures to record. The experimental effect is as follows: Figure 1-Figure 3 , Table 1 shows. The results showed that Bacillus subtilis JSNL1205 had obvious antagonistic effects on the three pathogenic bacteria. A P...

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Abstract

The invention discloses bacillus subtilis which is classified and designated as bacillus subtilis JSNL1205 and preserved in the China General Microbiological Culture Collection center, the preservation number is CGMCC No.6303, and the preservation date is June, 28th, 2012. The invention also discloses application of the bacillus subtilis in preventing and treating plant leaf spot diseases such asdendrobium candidum. The bacillus subtilis JSNL1205 has a suppression effect on various pathogenic fungi on the dendrobium candidum, can be artificially cultured and has the advantages of high propagation speed, convenience for large-scale production and good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a bacillus subtilis and its application in preventing and treating dendrobium candidum leaf spot. Background technique [0002] Biopesticides (biopesticides) include botanical pesticides, microbial pesticides, microbial secondary metabolites (antibiotics), etc., which are used to prevent and control various insects and diseases. One of the most concerned issues has become a research hotspot. With the general concern of the public on the safety of chemical pesticides in food and environment, the full use of beneficial microorganisms to control plant diseases has been paid more and more attention. Especially since entering the 21st century, governments and people of various countries have paid more attention to the development and application of biological pesticides. my country has also listed the research and development of microbial pesticides as a national ...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/125
Inventor 徐超张红岩席刚俊管斌赵桂华
Owner JIANGSU POLYTECHNIC COLLEGE OF AGRI & FORESTRY
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