Test method for vitamin B12 in milk and milk powder
A detection method and vitamin technology, applied in the direction of chemical reaction of materials for analysis, can solve the problems of complex detection process, cumbersome operation, cross-contamination, etc., and achieve good reproducibility of results, simple operation process, and avoid cross-interference. Effect
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Example 1
1. The milk processing method is as follows:
Add 1 mL of milk sample to a 50 mL centrifuge tube, accurately add 39 mL of distilled water or pure water, and vortex to obtain a solution. Filter and sterilize with a 0.2 μm sterile filter membrane in an ultra-clean bench to obtain a sterilized solution, and store the sterilized solution in a 1.5 mL sterile test tube. Among them, 0.22μm sterile filter membrane can be used according to needs, and there is no difference in use effect between the two compared with 0.2μm sterile filter membrane; at the same time, 2.0mL sterile test tube can be used according to need, and 1.5mL sterile test tube Comparing the two, there is no difference in the use effect.
The detection limit of the method is 0.03 μg / 100g, and the detection range is 0.03-0.18 μg / 100g.
2. The medium treatment method is as follows:
All operations in this step should be performed in a clean bench.
Add 10 mL of sterile water to the vitamin B12 medium, cov...
Example Embodiment
Example 1
1. The milk processing method is as follows:
Add 1 mL of milk sample to a 50 mL centrifuge tube, accurately add 39 mL of distilled water or pure water, and vortex to obtain a solution. Filter and sterilize with a 0.2 μm sterile filter membrane in an ultra-clean bench to obtain a sterilized solution, and store the sterilized solution in a 1.5 mL sterile test tube. Among them, 0.22μm sterile filter membrane can be used according to needs, and there is no difference in use effect between the two compared with 0.2μm sterile filter membrane; at the same time, 2.0mL sterile test tube can be used according to need, and 1.5mL sterile test tube Comparing the two, there is no difference in the use effect.
The detection limit of the method is 0.03 μg / 100g, and the detection range is 0.03-0.18 μg / 100g.
2. The medium treatment method is as follows:
All operations in this step should be performed in a clean bench.
Add 10 mL of sterile water to the vitamin B12 medium, cov...
Example Embodiment
Example 3
1. The milk processing method is as follows:
Add 1g of milk powder to a 50mL centrifuge tube, accurately add 39mL of distilled water or pure water, vortex and mix to obtain a solution. Filter and sterilize with a 0.22 μm sterile filter membrane in an ultra-clean bench to obtain a sterilized solution, and store the sterilized solution in a 2.0 mL sterile test tube.
The detection limit of the method is 0.03 μg / 100g, and the detection range is 0.03-0.18 μg / 100g.
2. The medium treatment method is as follows:
All operations in this step should be performed in a clean bench.
Add 10 mL of sterile water to the vitamin B12 medium, cover the bottle cap, and shake well to obtain the culture medium. Heat the culture medium in a water bath at 100°C for 20 minutes, shake it at least twice during the period (make sure the bottle is closed), and cool it down to room temperature (below 30°C) quickly. Use a 0.22 μm sterile filter membrane to filter and sterilize to obtain a ...
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