Animal cell fermentation and separation all-in-one machine

An animal cell and animal cell culture technology, applied in the field of an integrated machine for fermentation and separation of animal cells, can solve the problems of cumbersome operation, difficulty in realizing full utilization of animal cells, reducing the yield of supernatant liquid and production efficiency, etc., so as to improve production The effect of efficiency

Inactive Publication Date: 2012-11-07
苏州勃朗科技股份有限公司
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Problems solved by technology

Due to the strict requirements for the prevention and control of bacterial infection in biological fermentation engineering, such reciprocating operations are not only cumbersome to operate, especially frequent bacterial infection will lead to waste of raw materials, it is difficult to fully utilize animal cells in the best growth period, and the yield of supernatant is reduced. and production efficiency

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  • Animal cell fermentation and separation all-in-one machine
  • Animal cell fermentation and separation all-in-one machine

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Embodiment approach

[0012] Combined with the accompanying drawings:

[0013] 1) if figure 1 , when the hydraulic device is in the initial reset state, the 12 outer tank body is compounded with the content space of the 17 inner tank body, and the volume space is used as a space for cell culture. Through 1 gas filling port, 2 temperature sensor, 3 dissolved oxygen sensor, 4 pH sensor, 6 culture stirring motor, 7 mechanical seal, 8 sensor protection sheath, 9 safety valve, 10 sight glass, 20 material filling port, 21 Exhaust gas outlet, 22 Sampling port, 32 Insulation jacket liquid level gauge, 33 Insulation jacket, 35 Annular air distributor constitutes a dedicated system for cell culture reactors, and the cell culture is completed after about 7-11 days of culture cycle fermentation process. Among them, 1 gas filling port, 21 tail gas discharge port pneumatic valve, 2 temperature sensor, 3 dissolved oxygen sensor, 4 pH sensor, 6 cultivating stirring motor are controlled by computer program in 30 ...

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Abstract

Container equipment which can integrate an animal cell culture reactor and a separator is applied in experimental and production processes of animal cell culture and animal cell's metabolite extraction in the field of bio-fermentation engineering. The equipment is composed of an external tank with an elevating wall for adjusting volume and an internal tank with a fixed volume, and two work systems, namely an agitatory animal cell culture reactor and a separator, are configured. When the external tank and the internal tank are combined, the formed inner space can be used as a reactor to support the cell culture fermentation process under the control of an automation procedure. When the elevating wall is lifted by a hydraulic device, a separate disengagement chamber formed at the bottom of the tank is used to support the separation and extraction process under the control of the automation procedure. By the adoption of the equipment, cell metabolite can be extracted timely, continuously and conveniently, hygienic requirements can be guaranteed, and production efficiency can be raised.

Description

Technical field: [0001] The invention is a container device that can realize the integration of an animal cell culture reactor and a separator, which is applied to the experiment and production links of the cultivation of animal cells and the extraction of metabolites in the field of biological fermentation engineering, and can realize timely, continuous and convenient Extraction of cell metabolites (supernatant) to minimize the possibility of animal cell raw materials being infected by bacteria to ensure the fermentation effect and improve production efficiency. Background technique: [0002] At present, in the field of bio-fermentation engineering, the feasible acquisition of useful animal cell metabolites (referred to as "supernatant") is realized in two steps through two types of equipment, a cell culture reactor and a separator. The culture and fermentation of animal cell strains and medium (referred to as raw materials) through the reactor during a period of culture wi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/10
CPCC12M23/34C12M47/10C12M27/02
Inventor 李利昕
Owner 苏州勃朗科技股份有限公司
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