Gordon euryale seed extract with antioxidation and anti-ageing effects as well as preparation method and application thereof

A technology for anti-aging and extract, applied in the field of Gorgon Fructus extract and its preparation, can solve the problems of unstable strong oxidants and the like, and achieve the effects of less impurities, increased enzyme activity and good anti-oxidation

Inactive Publication Date: 2012-11-28
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nitric oxide synthase (NOS) can catalyze the generation of NO in the body. NO is not only a neurotransmitter, but also an extremely unstable and st

Method used

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  • Gordon euryale seed extract with antioxidation and anti-ageing effects as well as preparation method and application thereof
  • Gordon euryale seed extract with antioxidation and anti-ageing effects as well as preparation method and application thereof
  • Gordon euryale seed extract with antioxidation and anti-ageing effects as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0030] Example 1

[0031] A Gorgon extract with antioxidant and anti-aging effects, which is prepared by the following method:

[0032] (1) Take Gorgon medicinal materials, cleanly select, remove impurities, put them in a multifunctional extraction tank, add 8 times the amount of 80% ethanol, reflux and extract 3 times, each extraction for 8 hours, combine the extracts, filter, and obtain the filtrate , Concentrate under reduced pressure, evaporate to dryness, obtain the ethanol extract of Gorgon, for use.

[0033] (2) Take the ethanolic extract of Gorgon which was prepared in step (1) above, add water to suspend, extract 6 times with the same volume of ethyl acetate to obtain ethyl acetate extract, concentrate under reduced pressure, and evaporate to dryness to obtain Gorgon Ethyl acetate extract for use;

[0034] (3) Take the ethyl acetate extract prepared in step (2) on the D101 type macroporous adsorption resin, first eluting with water and 10% ethanol, and then eluting with 50%-...

Example Embodiment

[0035] Example 2

[0036] A Gorgon extract with antioxidant and anti-aging effects, which is prepared by the following method:

[0037] (1) Take Gorgon medicinal materials, cleanly select, remove impurities, put them in a multifunctional extraction tank, add 6 times the amount of 80% ethanol, reflux and extract 3 times, each extraction for 8 hours, combine the extracts, filter, and obtain the filtrate , Concentrate under reduced pressure, evaporate to dryness, obtain the ethanol extract of Gorgon, for use.

[0038] (2) Take the ethanolic extract of Gorgon which was prepared in step (1) above, add water and suspend it, extract 6 times with the same volume of n-butanol to obtain the n-butanol extract, concentrate under reduced pressure, and evaporate to dryness to obtain the Gorgon N-butanol extract, spare;

[0039] (3) Take the D101 type macroporous adsorption resin on the n-butanol extract prepared in step (2), and elute with water and 10% ethanol, and then with 50% to 60% ethanol. ...

Example Embodiment

[0040] Example 3 Anti-oxidation and anti-aging experiments

[0041] 1. Experimental materials

[0042] 1.1 Test drugs

[0043] Gorgon was purchased from Beijing Zhongge Lvyuan Technology Co., Ltd., batch number: 110901; the extracts prepared in Example 1 and Example 2 of the present invention.

[0044] D-galactose, Shanghai Baoman Biological Co., Ltd., batch number: 110523.

[0045] GSH-Px, NOS, AChE, Coomassie brilliant blue protein determination kit, Nanjing Jiancheng Institute of Bioengineering, batch number: 20120416.

[0046] 1.2 Experimental animals

[0047] Clean grade KM mice, provided by the Experimental Animal Center of Jiangsu University, animal certificate number: SCXK (Su) 2009-0002:.

[0048] 1.3 Experimental equipment

[0049] Mouse open field device, Shanghai Ruanlong Technology Development Co., Ltd.

[0050] SLY-WMS Morris water maze, Beijing Shuolinyuan Technology Co., Ltd.

[0051] FSH-2A adjustable high-speed electric homogenizer, Jintan Jinnan Instrument Factory.

[0052] ...

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Abstract

The invention discloses a gordon euryale seed extract with antioxidation and anti-ageing effects as well as a preparation method and application thereof. Through system optimization of an extraction solvent, an extracting method, an extraction method and a macroporous resin separation method, an optimal preparation process is obtained to prepare the gordon euryale seed extract. An experimental result shows that the gordon euryale seed extract provided by the invention is capable of obviously improving the motion capability and the exploratory behavior of a subacute ageing mouse in an open field, the academic record and memory ability of the mouse in water maze tests and the spleen and kidney indexes of the mouse, increasing the activity and protein contents of GSH-Px (Glutathione Peroxidase) and NOS (Nitric Oxide Synthase) in brain tissues, reducing the activity of AChE (acetylcholinesterase) in the brain tissues, improving the pathological change of the brain tissues, and having excellent antioxidation and anti-ageing effects.

Description

technical field [0001] The invention relates to an extraction method of a traditional Chinese medicine, in particular to a Gorgon fruit extract with anti-oxidation and anti-aging effects, a preparation method and application thereof. Background technique [0002] Gorgon fruit was first recorded in "Shen Nong's Materia Medica", and it was listed as top grade. The effect of spleen to relieve diarrhea, dispel dampness and stop leukorrhea, mainly treat nocturnal emission, slippery essence, enuresis, frequent urination, chronic diarrhea due to spleen deficiency, white turbidity, and leukorrhea. It is known as "ginseng in water" and "longan in water". It is a traditional Chinese medicinal material and Precious natural supplements. [0003] my country is one of the countries with the fastest aging rate in the world and one of the countries with the largest aging population. The national 1% population sample at the end of 2005 showed that the population over 65 years old accounted...

Claims

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Application Information

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IPC IPC(8): A61K36/62A61P39/06A61P9/10A61K31/352A61K131/00
Inventor 吴启南周婧乐巍段金廒沈蓓
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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