Method for excluding background interference of fruit and vegetable in rapid detection of fruit and vegetable pesticide residue and application thereof
A fast technology for pesticide residues, applied in the direction of biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problems of poor accuracy of detection results, detection interference, and inability to detect, etc., to achieve accurate detection results
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[0044] A method for eliminating the background color interference of fruits and vegetables in the rapid detection of pesticide residues in fruits and vegetables. The following is an example of lettuce with a darker background color. The specific operation steps are as follows:
[0045] (1) Push 5ml of the lettuce sample solution to be tested into the reactor immobilized with acetylcholinesterase, and carry out the enzyme inhibition reaction at 35°C for 15min;
[0046]The immobilization process of the enzyme in the reactor with acetylcholinesterase immobilized is as follows: the acetylcholinesterase is immobilized on 40-60 mesh large pore diameter cation exchange resin particles D113 (Anhui Wandong Resin Co., Ltd. Technology Co., Ltd.), and then loaded in the reactor; or use carbodiimide method to directly immobilize acetylcholinesterase on the inner surface of the activated reactor;
[0047] Described reactor is that diameter is 6mm, and height is the cylinder of 30mm, and the...
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