Method for excluding background interference of fruit and vegetable in rapid detection of fruit and vegetable pesticide residue and application thereof

A fast technology for pesticide residues, applied in the direction of biochemical equipment and methods, microbiological measurement/inspection, etc., can solve the problems of poor accuracy of detection results, detection interference, and inability to detect, etc., to achieve accurate detection results

Inactive Publication Date: 2012-11-28
赖智勇
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Problems solved by technology

Although the absorbance value caused by the color of the sample liquid has been deducted in the rapid detection of pesticide residues by the photometric method, the effective measurement limit of the photometer

Method used

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Examples

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Embodiment 1

[0044] A method for eliminating the background color interference of fruits and vegetables in the rapid detection of pesticide residues in fruits and vegetables. The following is an example of lettuce with a darker background color. The specific operation steps are as follows:

[0045] (1) Push 5ml of the lettuce sample solution to be tested into the reactor immobilized with acetylcholinesterase, and carry out the enzyme inhibition reaction at 35°C for 15min;

[0046]The immobilization process of the enzyme in the reactor with acetylcholinesterase immobilized is as follows: the acetylcholinesterase is immobilized on 40-60 mesh large pore diameter cation exchange resin particles D113 (Anhui Wandong Resin Co., Ltd. Technology Co., Ltd.), and then loaded in the reactor; or use carbodiimide method to directly immobilize acetylcholinesterase on the inner surface of the activated reactor;

[0047] Described reactor is that diameter is 6mm, and height is the cylinder of 30mm, and the...

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Abstract

The invention discloses a method for excluding background interference of fruit and vegetable in rapid detection of fruit and vegetable pesticide residues and the application thereof in the rapid detection of fruit and vegetable pesticide residues. The method for excluding background interference of fruit and vegetable in rapid detection of fruit and vegetable pesticide residues drains a colored fruit and vegetable sample liquid away after an enzyme inhibition reaction, so the colored fruit and vegetable sample liquid is not allowed to enter a color reaction system in the rapid detection, thus excluding the interference from fruit and vegetable background color for final detection results. As for the application of the method in the rapid detection of fruit and vegetable pesticide residues, an enzyme inhibit reaction, and an enzyme catalyzed substrate hydrolysis reaction are both conducted in a same closed reactor, so the impacts of environmental factor changes are also controlled at the lowest limit, making the detection results be relatively more accurate.

Description

technical field [0001] The invention relates to a method for eliminating background color interference of fruits and vegetables in the rapid detection of pesticide residues in fruits and vegetables and its application in the rapid detection of pesticide residues in fruits and vegetables, belonging to the field of rapid detection of pesticide residues in fruits and vegetables. Background technique [0002] The enzyme inhibition method has been used to detect the residues of organophosphorus and carbamate pesticides for more than 50 years. The basic principle is that organophosphorus and carbamate pesticides can inhibit the activity of acetylcholinesterase in the central and peripheral nervous systems of insects. According to this toxicological principle, the current rapid detection of organophosphorus and carbamate pesticide residues in fruits and vegetables mainly uses cholinesterase as the detection enzyme, and at the same time uses photometry to detect the degree of inhibit...

Claims

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Application Information

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IPC IPC(8): C12Q1/46C12Q1/44C12Q1/42C12Q1/26C12Q1/48
Inventor 赖智勇
Owner 赖智勇
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