Method for identifying chilli heat resistance by using detached leaves

A technology of detached leaves and heat resistance, applied in the field of vegetable breeding, can solve the problems of great influence of field identification environmental conditions, poor reproducibility of results, difficulty in large batches, etc., and achieve identification results that are consistent and repeatable with field natural identification results Good performance, improve the effect of the process

Inactive Publication Date: 2012-12-05
NORTHWEST A & F UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

[0004] Field identification is greatly affected by environmental conditions, the reproducibility of the results is poor, the time required is long, and the workload is heavy;
[0005] Artificial simulated adversity identification method requires certain facility conditions and is difficult to carry out in large quantities;
[0006] Physiological identification of adversity is an indirect identification method, and its reliability is poor
[0007] The above-mentioned existing identification methods are time-consuming, laborious and inefficient, especially for some strains and transgenic strains with few samples available for identification, and have great limitations.

Method used

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  • Method for identifying chilli heat resistance by using detached leaves

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Embodiment 1

[0035] 1. The heat-resistant pepper strain B35 was used as the heat-resistant control, and the heat-labile pepper strain B6 was used as the heat-sensitive control (Wei Zhu, Minghui Lu, Zhenhui Gong, and Rugang Chen. Cloning and Expression under Heat Stress of A Small Heat Shock Protein Gene CaHSP24 from Pepper[J].African Journal of Biotechnology,2011,10(25):4968-4976), the pepper strains used for detection were MC4, MITO LEE SELN-1 and 83-2018-2 (all cited from Asian Vegetables Development Research Center).

[0036] 2. Soak the seeds of capsicum material whose heat resistance is unknown, as well as the seeds of the heat-resistant control and the heat-sensitive control, respectively in warm water at 55°C for 20 minutes, soak in clear water for 5 hours, and accelerate germination in an artificial climate box at 28°C in total darkness; after 4 days Seeds are white, sown in plugs. Then culture them under the condition of 25℃~30℃ / 18℃, 16h / 8h, relative humidity 50%~70%, light 3000~...

Embodiment 2

[0055] 1. The heat-resistant pepper strain B35 was used as the heat-resistant control, and the heat-labile pepper strain B6 was used as the heat-sensitive control (Wei Zhu, Minghui Lu, Zhenhui Gong, and Rugang Chen. Cloning and Expression under Heat Stress of A Small Heat Shock Protein Gene CaHSP24 from Pepper[J].African Journal of Biotechnology,2011,10(25):4968-4976), the pepper strains used for detection are Marshal Huang No. 2 selection, Meiyu selection, Baozijiao and Early Calwonder (referred to as EC) (both materials are well-known in the art, provided by the applicant's inventor's research group).

[0056] 2. Soak the seeds of capsicum material whose heat resistance is unknown, as well as the seeds of the heat-resistant control and the heat-sensitive control, respectively in warm water at 55°C for 20 minutes, soak in clear water for 5 hours, and accelerate germination in an artificial climate box at 28°C in total darkness; after 4 days Seeds are white, sown in plugs. Th...

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Abstract

The invention discloses a method for identifying chilli heat resistance by using detached leaves. The method comprises the following steps that a chilli heat resistance strain B35 is used as heat resistance control, a chilli heat intolerant strain B6 is used as thermosensitive control selection, the method for identifying the chilli heat resistance of the detached leaves is adopted, the detached leaf heat harm grading standard and group heat harm grading standard are made, and the like. The method is characterized in that the chilli detached leaves are treated at the specific temperature and time, different heat resistance strains have obvious differences on the leaf expression symptoms, the heat harm index of a strain group to be detected is calculated on the basis, and the chilli heat resistance strain and the thermosensitive strain can be identified according to the made group heat damage grading standard. The method is particularly suitable for genetic analysis and heat resistanceidentification of precious chilli breeding plant strains including transgenosis plant strains and the like. The method belongs to the method with the advantages that the operation is simple, the speed is high, and the heat resistance of a large number of chilli seed resources is effectively identified. The chilli heat resistance breeding process can be greatly accelerated.

Description

technical field [0001] The invention belongs to the field of vegetable breeding, and specifically relates to a method for distinguishing different heat-resistant pepper germplasm resources by using isolated pepper leaves, in particular to identification of heat-resistant isolated pepper leaves and a heat-resistant grading system for isolated pepper leaves method of establishment. The method will not cause the death of heat-labile plants, and is convenient for saving seeds of heat-labile strains and genetic analysis of offspring, and is especially suitable for heat-resistant identification and genetic analysis of precious pepper breeding strains such as small strain populations and transgenic strains. It is a simple, rapid and effective method for identifying the heat resistance of a large number of pepper germplasm resources. Background technique [0002] Pepper (Capsicum annuum L.) is a typical "heat-loving and heat-labile" plant. Heat stress will occur if it exceeds 32°C,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G7/00
Inventor 巩振辉马宝鹏赵滢崔春梅郭猛逯明辉
Owner NORTHWEST A & F UNIV
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