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Herbicide-resistant sunflower plants with multiple herbicide resistant alleles of AHASL1 and methods of use

A sunflower and genetic technology, applied in the field of agriculture, can solve the problems of undescribed imidazolinone-specific resistance and other problems

Active Publication Date: 2012-12-05
BASF AGROCHEMICAL PROD BV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The mutations described there encode cross-resistance to imidazolinones and sulfonylureas, or specific resistance to sulfonylureas, but imidazolinone-specific resistance is not described

Method used

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  • Herbicide-resistant sunflower plants with multiple herbicide resistant alleles of AHASL1 and methods of use
  • Herbicide-resistant sunflower plants with multiple herbicide resistant alleles of AHASL1 and methods of use
  • Herbicide-resistant sunflower plants with multiple herbicide resistant alleles of AHASL1 and methods of use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0191] Example 1: Phenotypic interactions of imidazolinone resistance mutations in sunflower AHASL1

[0192] GM40 and GM1606 are mutant derived lines of sunflower that show a high level of tolerance to imidazolinones due to a point mutation at codon 122 of AHASL1 (Arabidopsis nomenclature) (WO2007005581 and Jul. 1, 2005 filed U.S. Provisional Patent Application Serial No. 60 / 695952). This demonstrates that the A122T mutation and derivative lines and hybrids homozygous for this mutation show better tolerance to imazamox than the known commercially available Clearfield sunflower homozygous for the A205V mutation on AHASL1 (WO2007005581) . Both mutants exhibit an incompletely dominant, sensitive allele relative to the wild type, as do many other examples in the literature. The present invention is based on the discovery that the A122T mutation exhibits near complete dominance over A205V for resistance to imidazolinones over the herbicide application range of 0.5X to 6X commerci...

Embodiment 2

[0196] Example 2: Response of homozygous A122T / A122T and A205V / A205V and heterozygous A122T / A205V events to imazapyr at the whole plant level

[0197] This experiment was performed to quantify and compare imazapyr in sunflower hybrids carrying the A122T and A205V mutations in homozygous (A122T / A122T or A205V / A205V) and heterozygous (A122T / A205V) states on different genetic backgrounds and at the whole plant level sensitivity.

[0198] Material

[0199] Seeds of different sunflower lines (Table 1) were obtained under field conditions.

[0200] Table 1. Sunflower materials utilized, their families, and types of mutation events.

[0201] the code family lineage Line (L) or Hybrid (H) mutation event L1 L A205V L2 L A205V H1 L1x L2 H A205V L3 cmsGM40 L A122T L4 L A122T H2 L3x L4 H A122T L5 BTK 47 L Sensitive H3 L3x L2 H A205V+A122T H4 L1x L4 H A...

Embodiment 3

[0236] Example 3: Herbicide tolerance of A122T and A205V homozygous and heterozygous lines and two mutant heterozygous lines (A122T / A205V) under field conditions

[0237] This experiment was carried out under field conditions to compare the difference between the mutants carrying the A122T and A205V mutations in homozygous (A122T / A122T or A205V / A205V), heterozygous (A122T / - or A205V / -) and double stacked heterozygous (A122T / A205V) states. Herbicide tolerance of genotyped sunflower hybrids and lines.

[0238] Material

[0239] The sunflower materials used are listed in Table 5.

[0240] Table 5: Item List

[0241]

[0242]

[0243] method

[0244] Seeds for each item in Table 5 were produced during the 2005-2006 growing season under optimal seed production conditions in South America. Field trials were conducted in 2006 at a site in North Dakota, USA. These items were arranged in randomized complete blocks using a split-plot design consisting of 3 replicates per ...

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Abstract

Herbicide resistant sunflower plants comprising two different herbicide-resistant alleles of the sunflower acetohydroxyacid synthase large subunit 1 (AHASL1) gene are described. Methods for making these sunflower plants and methods for controlling weeds or other undesired vegetation growing in the vicinity of these sunflower plants are disclosed. Such methods involve the use of acetohydroxyacid synthase-inhibiting herbicides. Methods for controlling parasitic weeds growing on sunflower plants are also described. Additionally provided are methods for determining the genotype of sunflower plants for AHASL1 gene.

Description

[0001] This application is a Chinese patent application 200880017562.X (International Application No. PCT / US2008 / 059125) divisional application. field of invention [0002] The present invention relates to the field of agriculture, in particular to herbicide-resistant sunflower plants comprising two different herbicide-resistant alleles of the sunflower acetohydroxyacid synthase large subunit 1 (AHASL1) gene. Background of the invention [0003] Acetohydroxyacid synthase (AHAS; EC4.1.3.18, also known as acetolactate synthase or ALS) is the first enzyme that catalyzes the biochemical synthesis of the branched-chain amino acids valine, leucine, and isoleucine (Singh (1999) "Biosynthesis of valine, leucine and isoleucine," in Plant Amino Acids, Singh, B.K., ed., Marcel Dekker Inc. New York, New York, pp. 227-247). AHAS is the site of action for four structurally and chemically distinct herbicide families including the sulfonylureas (Tan et al. (2005) Pest Manag. Sci. 61:246-57...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11A01H5/02A01M29/00A01M29/12
CPCC12N9/88A01H1/00A01H5/025C12N15/8278A01H5/02A01H6/14A01H1/123A01N43/50C12N15/8274
Inventor C·萨拉M·布洛斯M·埃查尔特B·K·辛格B·J·韦斯顿S·R·惠特
Owner BASF AGROCHEMICAL PROD BV
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