Creatine phosphokinase isozyme chemiluminescence immune quantitative detection kit and preparation method thereof

Abstract

The invention discloses a creatine phosphokinase isozyme chemiluminescence immune quantitative detection kit and a preparation method thereof. The kit comprises a creatine kinase-MB (CK-MB) antibody coated plate, a CK-MB antibody enzyme conjugate, a CK-MB calibrator, a CK-MB quality control product, a 20-times concentrated lotion, a light-emitting solution A and a light-emitting solution B. In addition, the invention also discloses the preparation method for the creatine phosphokinase isozyme chemiluminescence immune quantitative detection kit. Compared with the conventional kit, the kit provided by the invention is operated conveniently and safely, and does not pollute environment. Moreover, the kit also has the advantages that the concentration range of a detection sample is wide, the validity period of a reagent is long, the stability is high and the like.

Description

technical field

[0001] The invention relates to the field of immunoanalysis medicine, in particular to a creatine phosphokinase isoenzyme (CK-MB) chemiluminescent immunoquantitative detection kit and a preparation method thereof. Background technique

[0002] Creatine Kinase (CK) is a dimer composed of brain-type subunit (B) and muscle-type subunit (M), and has three isozymes CK-MM, CK-MB and CK-BB , CK exists in a large number of tissues such as cardiac muscle, skeletal muscle, and brain, and is an important kinase directly related to intracellular energy operation, muscle contraction, and ATP regeneration. CK-BB mainly exists in the brain, prostate, intestine, and lung; bone There are a large amount of CK-MM and a small amount of CK-MB in the muscle; although the myocardial tissue is dominated by CK-MM, the proportion of CK-MB is much higher than other tissues.

[0003] At present, myocardial injury, myocardial infarction and acute myocardial infarction (AMI) have become ...

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