Creatine phosphokinase isozyme chemiluminescence immune quantitative detection kit and preparation method thereof

A chemiluminescence immunoassay and creatine phosphokinase technology, applied in the field of immunoassay medicine, can solve the problems of low sensitivity and narrow detection range, and achieve the effects of high sensitivity, easy operation and good precision

Active Publication Date: 2012-12-05
BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The problem to be solved in the present invention is to provide a chemiluminescent immunoquantitative detection kit for blood creatine phosphokinase (Creatine Kinase, CK) and a preparation method thereof, which solves the defects of low sensitivity and narrow detection range

Method used

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  • Creatine phosphokinase isozyme chemiluminescence immune quantitative detection kit and preparation method thereof
  • Creatine phosphokinase isozyme chemiluminescence immune quantitative detection kit and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0033] Example 1: Preparation of creatine phosphokinase isoenzyme (CK-MB) chemiluminescent immunoquantitative detection kit

[0034] Creatine phosphokinase isoenzyme (CK-MB) chemiluminescent immunoquantitative detection kit, characterized in that the kit includes: CK-MB antibody-coated plate, CK-MB antibody enzyme conjugate, CK-MB calibration products, CK-MB quality control products, 20 times concentrated lotion, luminescent solution A and B.

[0035] Creatine phosphokinase isoenzyme chemiluminescent immunoquantitative detection kit was prepared by the following method

[0036]1) Preparation of CK-MB antibody-coated plate: Dilute CK-MB antibody to 1-10ug / mL with 0.05mol / L pH9.6 carbonate buffer, add it to a white microwell plate, and coat at 37°C for 2 hours; discard the liquid in the wells, wash the plate with pH 7.4 PBS-T buffer, then add phosphate buffer containing 0.5% BSA to seal the microwell plate, and block for 2 hours at 37°C; discard the liquid in the wells, and sha...

Embodiment 2

[0058] Example 2: Preparation of CK-MB Chemiluminescent Immunoquantitative Detection Kit

[0059] The kit of the present invention was prepared in the same manner as in Example 1, except that the solid phase carrier was a 48-well white microwell plate.

Embodiment 3

[0060] Embodiment 3: the using method of kit of the present invention

[0061] 1) Equilibrate the test kit at room temperature (18-25°C) for 30 minutes.

[0062] 2) Prepare lotion: Dilute the concentrated lotion with distilled water at 1:20 (1mL lotion plus 19mL distilled water). If the concentrated lotion has crystals, place the concentrated lotion at room temperature or 37°C until the crystals dissolve before diluting.

[0063] 3) Preparation of luminous liquid: 5 minutes before use, take an appropriate amount of luminous liquid A and B and mix them in equal volumes.

[0064] 4) Take out an appropriate amount of coated slats. Set 1 well for blank control, 2 wells for calibrator, and 10 wells for quality control. Add 20 μL each of CK-MB calibrator, quality control substance, and sample to each well, and do not add calibrator, quality control substance, and sample to the blank control.

[0065] 5) Add 100 μL of CK-MB enzyme conjugate to each well, except for blank control ...

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Abstract

The invention discloses a creatine phosphokinase isozyme chemiluminescence immune quantitative detection kit and a preparation method thereof. The kit comprises a creatine kinase-MB (CK-MB) antibody coated plate, a CK-MB antibody enzyme conjugate, a CK-MB calibrator, a CK-MB quality control product, a 20-times concentrated lotion, a light-emitting solution A and a light-emitting solution B. In addition, the invention also discloses the preparation method for the creatine phosphokinase isozyme chemiluminescence immune quantitative detection kit. Compared with the conventional kit, the kit provided by the invention is operated conveniently and safely, and does not pollute environment. Moreover, the kit also has the advantages that the concentration range of a detection sample is wide, the validity period of a reagent is long, the stability is high and the like.

Description

technical field [0001] The invention relates to the field of immunoanalysis medicine, in particular to a creatine phosphokinase isoenzyme (CK-MB) chemiluminescent immunoquantitative detection kit and a preparation method thereof. Background technique [0002] Creatine Kinase (CK) is a dimer composed of brain-type subunit (B) and muscle-type subunit (M), and has three isozymes CK-MM, CK-MB and CK-BB , CK exists in a large number of tissues such as cardiac muscle, skeletal muscle, and brain, and is an important kinase directly related to intracellular energy operation, muscle contraction, and ATP regeneration. CK-BB mainly exists in the brain, prostate, intestine, and lung; bone There are a large amount of CK-MM and a small amount of CK-MB in the muscle; although the myocardial tissue is dominated by CK-MM, the proportion of CK-MB is much higher than other tissues. [0003] At present, myocardial injury, myocardial infarction and acute myocardial infarction (AMI) have become ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/573
Inventor 刘萍宋启超董婷婷李克锦范利花
Owner BIOSCIENCE (TIANJIN) DIAGNOSTIC TECH CO LTD
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