Method for actual measurement of microcystis density and population size by using laser particle analyzer
A technology of laser particle size analyzer and Microcystis, which is applied in the field of resources and environment, can solve the problems of poor representativeness of evaluation results, difficult to count three-dimensional structures, and huge workload.
Inactive Publication Date: 2012-12-12
HOHAI UNIV
View PDF4 Cites 6 Cited by
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Microscopic observation will have certain limitations when studying the number of groups, group size, and number of cells in the group. Although the number of groups is easy to obtain by counting, the number of cells in each group is difficult to count due to the three-dimensional structure and mutual occlusion of the group cells.
When observing Microcystis colonies with a microscope, due to the small sampling volume (0.1μL-0.1mL) and the need for 1- to 10-fold dilution, the number and size of the colonies appearing in the field of view are quite random, which will cause Deteriorating the representativeness of the evaluation results
At the same time, 400-600 algae units must be counted for each sample when performing group evaluation, and the workload is also very huge
Microscopic observation plays an important role in the classification of phytoplankton and the biomass measurement of unicellular algae, but there are problems in operability and accuracy in the evaluation of the population characteristics of Microcystis in the form of colony
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View moreImage
Smart Image Click on the blue labels to locate them in the text.
Smart ImageViewing Examples
Examples
Experimental program
Comparison scheme
Effect test
Embodiment example
[0013] (1) Instruments and samples
[0014] The test adopts the Mastersize2000 laser particle size analyzer of British Malvern company.
[0015] Microcystis species were collected from the Institute of Hydrobiology, Chinese Academy of Sciences, cultured in a 250ml Erlenmeyer flask using BG-11 medium, and the light intensity was 5~50μE·m -2 ·s-1, culture temperature 25℃, light-dark ratio 12:12 hours. Under different light intensities and perturbation conditions, three types of samples with different population characteristics were obtained: the naked eye and microscope observation can clearly distinguish: type a samples are mainly single cells, a total of 24, and b samples are group-based. Main, a total of 13 samples, c samples of single cells, small populations and larger populations mixed, a total of 9 samples.
[0016] In June 2010, the sampling location was Gonghu Bay of Taihu Lake. The microcystis floating in the surface of the water where the microcystis was more obviou...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More PUM
Login to View More Abstract
The invention relates to a method for actual measurement of microcystis density and population size by using a laser particle analyzer. Through pretreatment of an on-site sample, rapid and simultaneous determination of microcystis density and population particle size distribution can be realized by using the laser particle analyzer. The method comprises the following concrete steps: 1, standing the sample for 12 h, collecting supernatant and supplementing distilled water with an amount equal to the amount of mixed liquid at bottom; 2, setting absorptivity of the laser particle analyzer to be 0.1, a refractive index to be 1.40 and a rotating speed of a circulating pump to be 1500 rad / min; and 3, adding clear water with a volume of V1 (600 to 700 mL) for scanning of a background value, adding the sample to be detected step by step, recording the volume V2 of the added sample and a corresponding shading degree OV when a shading degree reaches 10 to 20%, determining particle size distribution of microcystis and calculating microcystis density.
Description
technical field [0001] The invention relates to a method for actually measuring the density and group size of Microcystis by using a laser particle size analyzer. Through the use of this method, the density and population size distribution of Microcystis can be determined rapidly and simultaneously. Unlike some previous methods for measuring Microcystis density and colony size, this method can simultaneously measure Microcystis density and colony size without breaking the Microcystis colony or spending a lot of time using a microscope to measure Microcystis Colony size, this method can not only quickly obtain the density and population size of Microcystis, but also obtain the particle size distribution of Microcystis population. The invention belongs to the field of resource environment. Background technique [0002] Cyanobacteria blooms are generally characterized by a large number of reproduction and enrichment of Microcystis. The microcystis cell density in lakes is th...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More Application Information
Patent Timeline
Login to View More IPC IPC(8): G01N15/02
Inventor 朱伟李明李林罗永刚代晓炫肖曼郭丽丽
Owner HOHAI UNIV