Early warning method for chronic toxicity of water quality substance to Daphnia magna

A technology for chronic toxicity and early warning, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of insensitivity, high detection limit of toxicity, long test time, etc., to solve the acute toxicity detection sensitivity Poor performance, rapid detection, and the effect of solving the long-term detection cycle of chronic toxicity

Inactive Publication Date: 2012-12-19
TSINGHUA UNIV
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Problems solved by technology

Acute toxicity usually gives test results within 24 h or 48 h, but its toxicity detection limit is high, and the response to some samples with low concentrations of toxic substances (such as secondary effluent from u

Method used

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  • Early warning method for chronic toxicity of water quality substance to Daphnia magna
  • Early warning method for chronic toxicity of water quality substance to Daphnia magna
  • Early warning method for chronic toxicity of water quality substance to Daphnia magna

Examples

Experimental program
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Effect test

Embodiment 1

[0019] The secondary effluent of sewage plant A was taken, filtered with qualitative filter paper to remove suspended particles, and then used as water samples for the acute toxicity, chronic toxicity and enzyme activity detection experiments of Daphnia magna.

[0020] Firstly, the acute toxicity test of Daphnia magna was carried out according to the OECD202 method. The acute lethality rate of the water sample to Daphnia magna was 30% at 1 times the concentration for 48 hours. Therefore, the secondary effluent test concentration of the chronic toxicity and enzyme activity detection experiment was selected as 50%. , 75% and 1 times, using aerated and dechlorinated tap water to dilute accordingly, set 10 parallels for each concentration, and set tap water as a control, OECD 211 method for 21-day Daphnia magna chronic toxicity test: in 20 mL water samples Or put 1 Daphnia magna into tap water, culture it continuously for 21 days in an incubator with a light-to-dark ratio of 16:8 a...

Embodiment 2

[0026] Take reclaimed water from sewage plant B, use qualitative filter paper to remove suspended particles, and then use solid phase extraction to concentrate organic matter in the water to obtain a concentrated water sample equivalent to 200 times the concentration of reclaimed water, which is used for acute toxicity, chronic toxicity and enzyme Activity detection experiment.

[0027] Firstly, the acute toxicity test of Daphnia magna was carried out according to the OECD202 method. The 48-hour LC50 of the water sample was 24 times that of Daphnia magna. Therefore, the test concentration of regenerated water for the chronic toxicity and enzyme activity detection experiments was selected as 1, 2, 5 and 10 times. The concentration was set to 10 parallels, and tap water was set as the control, and the OECD 211 method was used to conduct the 21-day Daphnia magna chronic toxicity test: put 1 Daphnia magna in 20 mL of water sample or tap water, and set the light-dark ratio at 16:8, ...

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Abstract

The invention discloses an early warning method for chronic toxicity of a water quality substance to Daphnia magna, which belongs to the technical field of water pollution control. According to the invention, directed at a low-toxicity water quality situation, marker enzyme having sensitive response to toxicity in Daphnia magna is screened out, and potential of chronic toxicity within 21 days is predicted based on results of detection on a change rate of the activity of the marker enzyme in a short period when Daphnia magna is tainted. There are standard methods or commercial kits for detection of the marker enzyme, detection is convenient, rapid and accurate, detection requirements of rapid early warning are met, and the method provided by the invention is applicable to a single-substance water sample with a low concentration or to a complex water sample like reclaimed water and surface water containing a variety of components and having low toxicity and can rapidly reflect the level of biotoxicity.

Description

technical field [0001] The invention belongs to the technical field of water pollution control, and in particular relates to an early warning method for chronic toxicity of water quality substances to Daphnia magna. Background technique [0002] Daphnia magna is an internationally recognized standard organism for toxicity testing. Its acute toxicity and chronic toxicity tests have formed international standard methods and are widely used in the toxicity evaluation of pesticides, chemicals, industrial wastewater, domestic sewage and drinking water sources. Acute toxicity usually gives test results within 24 h or 48 h, but its toxicity detection limit is high, and the response to some samples with low concentrations of toxic substances (such as secondary effluent from urban sewage plants) is not sensitive; chronic toxicity The detection limit is lower, and it is more sensitive than acute toxicity, but the test takes a long time (21 days), and cannot reflect the water quality i...

Claims

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Application Information

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IPC IPC(8): C12Q1/46C12Q1/44C12Q1/34C12Q1/30C12Q1/26
Inventor 张薛李福志赵璇尹倩
Owner TSINGHUA UNIV
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