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Gingivitis and gingivitis ozostomia preventing mouthwash prepared by anti-porphyromonas gingivalis and IgY antibody with fusobacterium nucleatum specificity

A technology of Porphyromonas spp. and Fusobacterium nucleatum is applied in the field of oral products, which can solve the problems such as poor treatment effect of mouthwash, and achieve the effect of obvious effect.

Active Publication Date: 2013-01-09
上海美加净日化有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0012] The technical problem to be solved by the present invention is to provide a prophylactic treatment prepared by anti-Fusobacterium nucleatum and Porphyromonas gingivalis specific IgY antibodies against the problem that the existing periodontal disease drug mouthwash has a poor therapeutic effect. Mouthwash for gingivitis and bad breath caused by inoculum

Method used

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  • Gingivitis and gingivitis ozostomia preventing mouthwash prepared by anti-porphyromonas gingivalis and IgY antibody with fusobacterium nucleatum specificity
  • Gingivitis and gingivitis ozostomia preventing mouthwash prepared by anti-porphyromonas gingivalis and IgY antibody with fusobacterium nucleatum specificity
  • Gingivitis and gingivitis ozostomia preventing mouthwash prepared by anti-porphyromonas gingivalis and IgY antibody with fusobacterium nucleatum specificity

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Experimental program
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Effect test

Embodiment 1

[0020] Preparation of compound specific IgY antibody preparation against Porphyromonas gingivalis and Fusobacterium nucleatum:

[0021] A. Porphyromonas gingivalis and Fusobacterium nucleatum culture steps

[0022] A.1 Bacterial species: Porphyromonas gingivalis and Fusobacterium nucleatum;

[0023] A.2 Porphyromonas gingivalis (P.g) ATCC33277 strain and Fusobacterium nucleatum (Fn) ATCC10953 strain were taken, and the Porphyromonas gingivalis and Fusobacterium nucleatum (Fn) ATCC10953 strains were separated and cultured on BHI-S medium, respectively. bacilli, and were identified and confirmed as Porphyromonas gingivalis and Fusobacterium nucleatum to obtain Porphyromonas gingivalis cells and cell fragments, Fusobacterium nucleatum cells and cell fragments;

[0024] B. Immunogen preparation steps:

[0025] B.1 Take 10-70 parts by weight of Porphyromonas gingivalis cells and cell fragments, and 30-90 parts by weight of Fusobacterium nucleatum cells and cell fragments and mi...

Embodiment 2

[0036] Preparation of mixed specific IgY antibody preparation against Porphyromonas gingivalis and Fusobacterium nucleatum:

[0037] A. Porphyromonas gingivalis and Fusobacterium nucleatum culture steps

[0038] A.1 Bacterial species: Porphyromonas gingivalis and Fusobacterium nucleatum;

[0039] A.2 Porphyromonas gingivalis (P.g) ATCC33277 strain and Fusobacterium nucleatum (Fn) ATCC10953 strain were taken, and the Porphyromonas gingivalis and Fusobacterium nucleatum (Fn) ATCC10953 strains were separated and cultured on BHI-S medium, respectively. bacilli, and were identified and confirmed as Porphyromonas gingivalis and Fusobacterium nucleatum to obtain Porphyromonas gingivalis cells and cell fragments, Fusobacterium nucleatum cells and cell fragments;

[0040]B. Immunogen preparation steps:

[0041] B.1 Take 10-70 parts by weight of Porphyromonas gingivalis cells and cell fragments, and 30-90 parts by weight of Fusobacterium nucleatum cells and cell fragments and mix th...

Embodiment 3

[0052] Separately take Porphyromonas gingivalis cells and cell fragments as bacterial liquid; prepare anti-Porphyromonas gingivalis specific IgY extracts separately. Refer to Example 1 for the rest of the preparation.

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Abstract

The invention discloses IgY mouthwash prepared by anti-porphyromonas gingivalis and an IgY antibody with fusobacterium nucleatum specificity, which is used for preventing gingivitis and gingivitis ozostomia. The IgY mouthwash comprises an IgY preparation for resisting periodontal pathogens and basic mouthwash materials, wherein the IgY preparation for resisting periodontal pathogens is prepared by raw materials according to the following mass percent accounting for the total mass of toothpaste: 0.01%-0.5% of anti-porphyromonas gingivalis and IgY antibody preparation with fusobacterium nucleatum specificity, 0.01-2.0% of chlorhexidine, 1.0-30.0% of glycerol, and 0.1-2.0% of polyvinylpyrrolidone, wherein a protective preparation of the anti-porphyromonas gingivalis and the IgY antibody preparation with fusobacterium nucleatum specificity is prepared by the chlorhexidine, the glycerol, and the polyvinylpyrrolidone. The IgY mouthwash which is disclosed by the invention and is used for treating and preventing gingivitis and ozostomia has an obvious effect on treating and preventing the gingivitis and the ozostomia.

Description

technical field [0001] The invention relates to the technical field of oral products, in particular to a mouthwash prepared by using specific IgY antibodies against Fusobacterium nucleatum and Porphyromonas gingivalis to prevent gingivitis and bad breath caused by the two bacteria. Background technique [0002] The incidence of gingival and periodontal diseases accounts for more than 70% of the population in my country, and Porphyromonas gingivalis is the main suspected cause of periodontal diseases such as chronic gingivitis, chronic periodontitis and aggressive periodontitis bacteria, whose pili can bind to glyceraldehyde-3-phosphate dehydrogenase on the surface of oral epithelial cells, invade gingival epithelial cells and gingival fibroblasts, and escape immune clearance. Porphyromonas gingivalis produces dipeptidyl aminopeptidase to degrade connective tissue and promote increased MMP-2 and MMP-1 activity. The arginine- and lysine-specific cysteine ​​proteases (RgpA, Rgp...

Claims

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Application Information

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IPC IPC(8): A61K8/81A61K8/64A61Q11/00A61P1/02A61P31/04
Inventor 陈健芬罗鑫龙孙晓青张萍
Owner 上海美加净日化有限公司
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