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Strain for highly yielding enramycin, and preparation method and application thereof

A technology for enramycin and high-yielding strains, which is applied in the field of high-yielding enramycin strains and the preparation thereof, can solve the problems of limited improvement of strain production capacity, single screening method, etc., so as to prevent the infiltration of antibiotics and block ultraviolet rays. , the effect of increasing the yield of enramycin

Active Publication Date: 2013-11-06
HEBEI SHENGXUE DACHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although this method has improved the production capacity of bacterial strain to a certain extent, because only adopted chemical mutagenesis, and screening mode is single, so the improvement of bacterial strain production capacity is limited

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1 Screening of Enramycin High-Producing Strains

[0023] a. Preparation of slant medium: Weigh 8g maltose powder, 0.8g dry yeast powder, 0.8g tuna extract, 1g tryptone, 3g sodium chloride, 20g agar, add water to 1000ml, and then use 30% by mass % NaOH aqueous solution to adjust the pH to 6.7±0.1 to obtain a mixed solution A. Take the above mixture A, divide it into several 30 mm×200 mm test tubes, add cotton plugs, two layers of gauze, and two layers of kraft paper in sequence, and sterilize at 0.11±0.05MPa, 121±1°C for 20 min . After the sterilization is completed, shake it well immediately, and when the temperature is lowered until it is not hot to the touch, spread it on a slant, and obtain a slant medium after solidification.

[0024] Preparation of spore suspension Ⅰ: Take the starting strain of Streptomyces fungicide (4.1312), inoculate it on the above slant medium, cultivate it at 28.0±0.5°C and relative humidity of 40-60% for 7 days, then add 3 mL of s...

Embodiment 2

[0032] Example 2 Retesting and Stability Investigation of Enramycin High-Producing Strains

[0033] Strain re-test: Take the sand tube preserved DCS067 in Example 1, inoculate it on the slant medium prepared in Example 1, cultivate it for 7 days at 28.0±0.5°C and a relative humidity of 40-60%, and then dig out about 0.5×0.5cm 2 The bacterial layer was inoculated onto the seed medium prepared in Example 1, and cultured at 28.0±0.5°C and shaken at 220 rpm for 26-30 h, then transferred to the fermentation medium prepared in Example 1, and incubated at 28.0±0.5°C , cultured with shaking at 220 rpm for 10 days, and the titer of enramycin in the fermentation broth was determined, and the results were 10079, 11034, 10604 U / mL, with an average of 10572 U / mL. This result indicated that the DCS067 strain had good activity and had the characteristic of high enramycin production.

[0034] Stability investigation: take the sand tube preserved in DCS067 in Example 1, inoculate it on the a...

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Abstract

The invention discloses a strain for highly yielding enramycin, simultaneously provides a screening method of the strain, and provides application of the strain in enramycin production. The preserving number of the strain DCS067 for highly yielding enramycin is CGMCCONO: 6220, the preserving unit is China General Microbiological Culture Collection Center (CGMCC), and the preserving date is June 14, 2012. The DCS067 strain enables production rate of enramycin to be improved by 47.80%.

Description

technical field [0001] The invention relates to a bacterial strain for producing antibiotics, in particular to a high-yield enramycin-producing bacterial strain and its preparation method and application. Background technique [0002] Enramycin is a cyclic polypeptide antibiotic produced by the fermentation of Streptomyces fungicidious, which is composed of more than a dozen amino acids and unsaturated fatty acids. In the past, most of the fungicidal Streptomyces strains used to produce enramycin were screened from wild strains, and their production levels were low. Chinese patent ZL 201010528367.2 discloses a mutant strain of fungicidal Streptomyces FYFJ03 (preservation number CGMCC No.4113). Staphylococcus, the mutant strains were screened according to the biological potency method, and a strain with high production of enramycin was obtained. Although this method improves the production capacity of the bacterial strain to a certain extent, because only chemical mutagenes...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12N13/00C12N15/01C12P21/04C12R1/465
Inventor 王雪峰闫彩洁刘卫哲
Owner HEBEI SHENGXUE DACHENG PHARMA
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