Method for molecular-marker-assisted selection of cowpea drought tolerant variety

A molecular marker-assisted, molecular marker technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve the problems of time-consuming and laborious, a large amount of land and human resources, and achieve reduced workload, low cost, and high capacity. stable effect

Active Publication Date: 2013-01-09
上海凌恩生物科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a time-saving and labor-saving method for cowpea conventional breeding methods that rely solely on fields or greenhouses for drought-resistant identification at the seedling stage or adult plant stage, which require a large amount of land and human resources and are time-consuming and labor-intensive. , Accurate and rapid molecular marker-assisted breeding method for drought-tolerant varieties of cowpea

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  • Method for molecular-marker-assisted selection of cowpea drought tolerant variety
  • Method for molecular-marker-assisted selection of cowpea drought tolerant variety

Examples

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Effect test

Embodiment 1

[0024] Example 1: (Marker-assisted selection of cowpea line 'ZJ106' and "Fandou" hybrid backcross progeny)

[0025] (1) Hybridization and backcrossing of non-drought-tolerant main varieties and drought-tolerant resources: select plump non-drought-tolerant recurrent parents ('ZJ106', good agronomic traits, weak drought tolerance) and drought-tolerant donor parents (" rice bean", strong drought tolerance, but poor agronomic traits) sowed in the field, hybridized after flowering, harvested seeds after the pods mature, which is F 1 ; The next year will be F 1 (about 5 grains are enough) and reincarnated parent seeds are sown in the field, hybridized after flowering, and the seeds are harvested after the pods mature, which is BC 1 f 1 ; the third year will be BC 1 f 1 (about 30 seeds) and reincarnated parent seeds are sown in the field, hybridized after flowering, and the seeds are harvested according to the line after the pods mature, which is BC 2 f 1 , each line receiv...

Embodiment 2

[0031] Example 2: (Molecular marker-assisted selection of backcross progenies of cowpea lines 'Tezao 30' and "314")

[0032] The drought-tolerant cowpea line "314" has a DNA sequence completely identical to that of "rice bean" on the amplified fragment of marker 1_1286CAPS, so it can be used for marker-assisted selection by marker 1_1286CAPS.

[0033] (1) Hybridization and backcrossing of non-drought-tolerant main varieties and drought-tolerant resources: select plump non-drought-tolerant recurrent parents ('Tezao 30', good agronomic traits, weak drought tolerance) and drought-tolerant donor parents ("314", strong drought tolerance, but poor agronomic traits) sown in the field; hybridize after flowering, collect seeds after the pods mature, which is F 1 ; The next year will be F 1 (about 5 grains are enough) and reincarnated parent seeds are sown in the field, hybridized after flowering, and the seeds are harvested after the pods mature, which is BC 1 f 1 ; the third...

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Abstract

The invention discloses a method for molecular-marker-assisted selection of a cowpea drought tolerant variety and belongs to the technical field of vegetable biotechnological breeding. The method includes screening of a cowpea drought tolerant association single nucleotide polymorphism (SNP) molecular marker site 1_1286, conversion, amplification and enzyme digestion of SNP to cleaved amplified polymorphic sequence (CAPS) markers, preparation of cowpea materials to be detected, molecular marker detection of seedling drought tolerance of cowpea materials to be detected, phenotype redetection of stay-green Stg of stubbing stems to be detected and agronomic characters and the like. The method has the advantages of being simple and practical, rapid and flexible, accurate in result, low in cost and the like. A molecular marker detection process only needs 4 to 5 hours, and the cost is only about twentieth of that of traditional phenotypic identification. The method can be promoted and applied in vegetable breeding and seed identification sectors.

Description

technical field [0001] The invention belongs to the field of crop breeding biotechnology, in particular to a method for assisting breeding of cowpea drought-tolerant varieties by using molecular markers. Background technique [0002] Long cowpea is an important summer bean vegetable in my country and is deeply loved by the people. However, cowpea is mainly planted in the hot and dry seasons of summer and autumn, and the production of cowpea in the whole country, especially in the north, is facing the great threat of drought. Drought stress will cause a large number of cowpea to shed flowers and pods and form "ratized" pods, resulting in huge yield loss and serious quality decline. At present, the majority of cowpea growers have an urgent desire for high-quality long cowpea varieties with strong drought tolerance (Agbicodo et al., 2009). Therefore, improving the drought tolerance of cultivated long cowpea is one of the major problems to be solved urgently in the breeding of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
Inventor 徐沛李国景吴晓花汪宝根黄芸萍鲁忠富王毓洪王莎
Owner 上海凌恩生物科技有限公司
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