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Method for preparing pogosterol

A technology of bogosterol and methanol, which is applied in the field of preparation of bogosterol, can solve the problems of less research on bogosterol, and achieve the effects of maintaining stability, simple process and high yield

Inactive Publication Date: 2013-01-16
NANJING ZELANG AGRI DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It mainly exists in the leaves of Vernonia Vernonia pogosperma, a plant of Compositae. Modern pharmacological studies have shown that pogosterol has certain cytotoxic activity, and it is weak against L-1210 cells in vitro. At present, there are few studies on bogosterol. Therefore, a high-efficiency, environmentally friendly, simple and suitable preparation method for industrial production is needed to provide a wealth of information for further research on the active ingredients in Vernonia bogosterol and bogosterol. raw material

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take the dried leaves of Vernonia vernonia and pulverize them with a pulverizer, weigh 10kg of the powder, add 12 times the weight of the medicinal material in 85% methanol solution, soak and extract at room temperature for 8 hours, filter, repeat the above operation on the filter residue, and extract twice, combine the filtrate, reduce Concentrate under pressure to form an extract to obtain a methanol extract; take the extract and add 2 times the volume of water to disperse, then extract twice with dichloromethane equal to the volume of water, combine the dichloromethane layers, and recover the dichloromethane under reduced pressure (for the next extraction), continue to extract the water layer with water-saturated n-butanol for 3 times, separate the upper layer, namely the n-butanol layer, combine the n-butanol layer, and recover n-butanol under reduced pressure (for the next extraction) ) to obtain total steroidal saponins; add an appropriate amount of water to total ...

Embodiment 2

[0017] Take the dried leaves of Vernonia vernonia and pulverize them with a pulverizer, weigh 10kg of the powder, add 10 times the weight of the medicinal materials to 100% methanol solution, soak and extract at room temperature for 5 hours, filter, repeat the above operation on the filter residue, and extract once more, combine the filtrate, reduce Compress and concentrate into an extract to obtain a methanol extract; take the extract and add 2.5 times the volume of water to disperse, then extract twice with dichloromethane equal to the volume of water, combine the dichloromethane layers, and recover the dichloromethane under reduced pressure (for the next extraction), continue to extract the water layer with water-saturated n-butanol for 3 times, separate the upper layer, namely the n-butanol layer, combine the n-butanol layer, and recover n-butanol under reduced pressure (for the next extraction) ) to obtain total steroidal saponins; add an appropriate amount of water to tot...

Embodiment 3

[0019] Take the dried leaves of Vernonia vernonia and pulverize them with a pulverizer, weigh 10kg of powder, add 6 times the weight of medicinal materials in 90% methanol solution, soak and extract at room temperature for 10 hours, filter, repeat the above operation for the filter residue, and extract twice, combine the filtrate, reduce Concentrate under pressure to form an extract to obtain a methanol extract; take the extract and add 1.5 times the volume of water to disperse, then extract twice with dichloromethane equal to the volume of water, combine the dichloromethane layers, and recover the dichloromethane under reduced pressure (for the next extraction), continue to extract the water layer with water-saturated n-butanol for 3 times, separate the upper layer, namely the n-butanol layer, combine the n-butanol layer, and recover n-butanol under reduced pressure (for the next extraction) ) to obtain total steroidal saponins; add appropriate amount of water to total steroid...

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Abstract

The invention discloses a method for preparing pogosterol. The method obtains pogosterol with purity more than 95% by methods of methanol immersion, organic solvent extraction and enzymolysis, high speed counter-current chromatography, recrystallization, etc. The method does not need operations of column chromatography or synthesis, has the characteristics of simple process, high efficiency, mild separation environment, high product purity, and the like, is suitable for industrial production, and lays the firm foundation for extraction of effective components in vernonia pogosperma.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine chemistry, and in particular relates to a method for preparing bogosterol. Background technique [0002] Pogosterol (Pogosterol) CAS registration number is 149155-26-0, molecular formula is C 29 h 46 o 6 , with a molecular weight of 490.68, is a steroidal saponin, mp.154-157°C. Mainly found in the Compositae (Compositae) plant Vernonia pogo Vernonia pogosperm In the leaves of a, modern pharmacological studies have shown that bogosterol has a certain cytotoxic activity, and it has a weak cytotoxic effect on L-1210 cells in vitro. At present, there are few studies on bogosterol, so a high-efficiency, environmentally friendly The preparation method is simple, suitable for industrial production, and provides abundant raw materials for the further research on the active ingredients in Vernonia bogo and bogosterol. Contents of the invention [0003] The object of the present invention ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J71/00
Inventor 苏刘花
Owner NANJING ZELANG AGRI DEV
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