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Extraction process of polysaccharide in portulaca oleracea

An extraction process, the technology of purslane, applied in the field of extraction process of polysaccharides in purslane, can solve the problems of low extraction rate and long time consumption

Inactive Publication Date: 2013-01-16
张羚慧
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the polysaccharides of purslane are usually extracted by water extraction and microwave-assisted extraction. However, these two methods are time-consuming and the extraction rate is not high.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] 1. Pretreatment

[0019] 2. Degreasing

[0020] Use anhydrous ether in Soxhlet, reflux and degrease at 50°C for 3 hours to obtain degreased purslane powder;

[0021] 3. Extraction

[0022] First, distilled water was added to the degreased purslane powder, the ratio of solid to liquid was 1:15, ultrasonic extraction was carried out at 80°C for 25 minutes, and the ultrasonic power was 240w;

[0023] 4. Suction filtration, decolorization and concentration

[0024] 5. Deproteinization

[0025] Dissolve the crude polysaccharide in double distilled water, mix well and add the mixed solution of chloroform and n-butanol according to the mass ratio of 5:1, the m 氯仿 :m 正丁醇 =5:1, take the supernatant after shaking, add 4 times the volume of ethanol with a mass fraction of 95%, put it in the refrigerator and let it stand for 24 hours, and then obtain the deproteinized purslane polysaccharide by centrifugation;

[0026] 6. Washing.

Embodiment 2

[0028] 1. Pretreatment

[0029] 2. Degreasing

[0030] Use anhydrous ether in Soxhlet, reflux and degrease at 50°C for 3 hours to obtain degreased purslane powder;

[0031] 3. Extraction

[0032] First, distilled water was added to the degreased purslane powder, the ratio of solid to liquid was 1:20, ultrasonic extraction was carried out at 60°C for 35 minutes, and the ultrasonic power was 200w;

[0033] 4. Suction filtration, decolorization and concentration

[0034] 5. Deproteinization

[0035] Dissolve the crude polysaccharide with double-distilled water, mix well and add the mixed solution of chloroform and n-butanol according to the mass ratio of 4:1, the mixed solution contains m 氯仿 :m 正丁醇 =5:1, take the supernatant after shaking, add 5 times the volume of ethanol with a mass fraction of 95%, put it in the refrigerator and let it stand for 24 hours, and then obtain the deproteinized purslane polysaccharide by centrifugation;

[0036] 6. Washing.

Embodiment 3

[0038] 1. Pretreatment

[0039] 2. Degreasing

[0040] Use anhydrous ether in Soxhlet, reflux and degrease at 50°C for 3 hours to obtain degreased purslane powder;

[0041] 3. Extraction

[0042] First, distilled water was added to the degreased purslane powder, the ratio of solid to liquid was 1:25, ultrasonic extraction was carried out at 70°C for 30 minutes, and the ultrasonic power was 280w;

[0043] 4. Suction filtration, decolorization and concentration

[0044] 5. Deproteinization

[0045] Dissolve the crude polysaccharide in double-distilled water, and after mixing, add the mixed solution of chloroform and n-butanol according to the mass ratio of 4.5:1. In the mixed solution, m 氯仿 :m 正丁醇 =5:1, take the supernatant after shaking, add 3 times the volume of ethanol with a mass fraction of 95%, put it in the refrigerator and let it stand for 24 hours, and then obtain the deproteinized purslane polysaccharide by centrifugation;

[0046] 6. Washing.

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Abstract

Relating to an extraction process of polysaccharide, the invention specifically relates to an extraction process of polysaccharide in portulaca oleracea. The extraction process of polysaccharide in portulaca oleracea provided in the invention is characterized by short time and high extraction rate. The process includes the technological steps of: pretreatment and degreasing; extraction: first adding distilled water into degreased portulaca oleracea powder, with the powder and the water in a ratio of 1:15-1:25, conducting ultrasonic extraction for 25-35min under a temperature of 60-80DEG C and ultrasonic power of 200-280w; pumping filtration, decolorization, and concentration; deproteinization: using double distilled water to dissolve crude polysaccharide, mixing them evenly, and adding the mixture into a mixed solution of chloroform and n-butanol according to a mass ratio of 4:1-5:1, with the m chloroform and the m n-butanol of the mixed solution being in a ratio of 5:1, shaking the newly obtained mixture and taking the supernatant, adding ethanol with a volume of 3-5 times and a mass fraction of 95%, placing the mixture in a refrigerator to stand for 24h, then performing centrifugation so as to obtain the deproteinized portulaca oleracea polysaccharide; and conducting washing.

Description

Technical field: [0001] The invention relates to a polysaccharide extraction process, more specifically, relates to a polysaccharide extraction process in purslane. Background technique: [0002] Purslane is an annual succulent herb of the family Portulaca oleracea. It has green leaves, red stems, yellow flowers, white roots, and black seeds, so it is also called "five elements grass". It is widely distributed in temperate and tropical regions, and is the most common in my country. Purslane is rich in nutrients and a variety of functional factors, among which polysaccharides are the main functional components contained in purslane, which has various physiological activities such as immune regulation, anti-tumor, anti-virus, anti-infection, and hypoglycemia. At present, water extraction and microwave-assisted extraction of purslane polysaccharides are commonly used, but these two methods take a long time and the extraction rate is not high. Invention content: [0003] The ...

Claims

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Application Information

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IPC IPC(8): C08B37/00
Inventor 张羚慧
Owner 张羚慧
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