Pollution-free sterilizing system for air intake and material feeding of bioreactors and control method thereof

The technology of a bioreactor and a sterilization system is applied in the field of non-polluting sterilization systems of bioreactors, which can solve the problems of shortening the service life of the filter element, the temperature is not stable enough, and the filter element is damaged, and achieves the effect of prolonging the service life.

Active Publication Date: 2013-01-23
诗华动保科技(北京)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Second, the feeding pipeline and the reactor must be sterilized by pure steam at the same time, and the feeding pipeline needs to be sterilized before each feeding, so the feeding can only be fed once during a fermentation process, which limits the scope of use of the reactor
[0008] Third, pure steam is not stable enough in pressure and temperature, and it is difficult to control. When pure steam is used to sterilize the filter element of the intake filter, it will cause certain damage to the filter element, thereby shortening the service life of the filter element

Method used

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  • Pollution-free sterilizing system for air intake and material feeding of bioreactors and control method thereof
  • Pollution-free sterilizing system for air intake and material feeding of bioreactors and control method thereof

Examples

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Effect test

Embodiment 1

[0064] In a preferred embodiment of the present invention, as figure 2 As shown, a pollution-free sterilization system for bioreactor air intake and feeding is provided, including: a bioreactor 12, an air intake (steam) device and a feeding device that are respectively connected to the bioreactor 12; wherein , the intake (steam) device includes an intake (steam) pipeline 23, an intake (steam) valve 9 and an intake filter 10, and the intake filter 10 is located between the intake (steam) valve 9 and the bioreactor 12 Between; feeding device includes feeding pipeline 21, exhaust valve 6 and union 30, and union 30 connects feeding pipeline 21 and exhaust valve 6; between bioreactor 12 and intake filter 10 A three-way device 22 is provided, and the three-way device communicates with the bioreactor 12, the intake filter 10 and the pure steam inlet and exhaust branches; the three-way device 22 contains three channel valves, wherein the first channel valve 22a is connected to the i...

Embodiment 2

[0068] In a preferred embodiment, the pipe opening connecting the third channel valve 34c of the three-way structure 34 and the bioreactor 12 is arranged at the top of the bioreactor 12 . Embodiment 2 Various control or application methods of the pollution-free sterilization system for the bioreactor air intake and feeding of the present invention

[0069] When normal high-pressure sterilization of the culture medium of the bioreactor is required, the inlet valve 9, inlet filter 10, valves 22a and 22b are opened, valves 5, 22c, and 34a are closed, and pure steam enters through the inlet filter. In the bioreactor 12, the valves 34b, 34c and 6 are opened, and the steam is exhausted through the steam exhaust branch.

[0070] If the online detection of the filter element of the intake filter fails due to high pressure due to the number of uses, pressure, etc., then close the valve 22b, open the intake valves 9, 22a, 22c and the exhaust valve 5, the intake device and the bioreactor...

Embodiment 3

[0076] Embodiment 3 Application of the bioreactor air intake and feeding of the present invention uses a pollution-free sterilization system to sterilize the medium in the bioreactor

[0077] 1. Empty elimination

[0078] Refers to the purging and disinfection of the bioreactor, the filter housing of the intake filter (the intake filter does not add a filter element, only the filter housing), the exhaust valve and all pipelines, and the order of steam intake is intake filter filter housing, bioreactor, exhaust valve and all pipelines. When the temperature of the reactor rose to 121°C, the timing was started for emptying. At this time, do not add air intake filter element, pH probe, dissolved oxygen probe, and do not start stirring. Under the condition that the temperature is kept constant, all the pipelines (including feeding pipes, exhaust pipes, etc.) are purged and sterilized with pure steam. After disinfection, close all valves and wait for use.

[0079] Second, the ac...

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Abstract

The invention discloses a pollution-free sterilizing system for air intake and material feeding of bioreactors and a control method of the system. The system is provided with a three-way device (22) between the bioreactor (12) and an air intake filter (10); the three-way device is communicated with the bioreactor (12), the air intake filter (10) and clean steam intake and exhaust branches; the other three-way device (34) is arranged between a movable joint (30) and the bioreactor (12); the three-way device is communicated with the bioreactor (12), a material feeding pipe (21) and the clean steam intake branch. The air intake device, the material feeding device and the bioreactor (12) of the system do not sterilize synchronously by arrangement of two three-way devices so as to effectively prolong the service life of filter elements and reduce waste of culture mediums; furthermore, the system can feed material and sterilize at any time during a fermentation process without influence for the fermentation process so as to enlarge the application range.

Description

technical field [0001] The invention relates to a pollution-free sterilization system used in biotechnology, in particular to a pollution-free sterilization system for bioreactor air intake and feeding, which belongs to the pollution-free sterilization system of a bioreactor. Background technique [0002] A bioreactor is a device system that uses the biological functions of enzymes or organisms (such as microorganisms) to perform biochemical reactions in vitro. It is a biological function simulator, such as a fermenter, immobilized enzyme or immobilized cell reactor Wait. The function of the bioreactor is to provide an optimized physical and chemical environment for the metabolism of organisms, so that they can grow faster and better, so as to obtain more required biomass or metabolites. [0003] Only by ensuring that the environment in the bioreactor meets the requirements for the reproduction of organisms can more biomass or metabolites be obtained. For this reason, the i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/12
Inventor 杨国良梁爽张洪郑杰王文泉
Owner 诗华动保科技(北京)有限公司
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