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Nanogold-labeled chip for polygene diagnosis of prostate cancer

A prostate cancer and nano-gold technology, applied in the field of gene chips, can solve problems such as huge investment, difficulty in combining nano-gold and probes, long-term funding, etc., and achieve the effect of reducing inspection costs

Inactive Publication Date: 2013-01-23
广州市第一人民医院 +3
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, nano-gold labeling chip technology has difficulties in combining nano-gold and probes, and it is difficult to master the concentration of nano-gold and the color development conditions. The development process of immunoassay and other rapid detection technologies using nano-gold as a marker requires huge investment and relatively long time. long time

Method used

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  • Nanogold-labeled chip for polygene diagnosis of prostate cancer
  • Nanogold-labeled chip for polygene diagnosis of prostate cancer
  • Nanogold-labeled chip for polygene diagnosis of prostate cancer

Examples

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Embodiment 1

[0035] The nano-gold marker chip for multi-gene diagnosis of prostate cancer of the present invention comprises 11 genes (IGF1, P27, AR, PSMA) with significantly different expressions in prostate cancer and benign prostatic hyperplasia obtained after screening by gene chip and verified by fluorescent quantitative PCR. , KLK3, PCNA, Ki-67, KLK1, KLK2, TMPRSS2, SREBF2), prostate cancer cell line RNA, nano-gold-labeled oligonucleotide probe, and a chip made of nitrocellulose. This method is based on the hybridization between the biotin-labeled target sequence and the immobilized probe on the chip, through the affinity effect of biotin-streptavidin, the target sequence is bound to nano-gold, and then silver-stained by nano-gold catalysis The magnification effect produces a recognition signal visible to the naked eye.

[0036] The specific production method of the chip:

[0037] 1 Biotin-labeled target sequence preparation

[0038] 1.1 RNA extraction

[0039] 1.) After adding Tr...

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Abstract

The invention discloses a nanogold-labeled chip for polygene diagnosis of prostate cancer. The nanogold-labeled chip mainly comprises 11 genes, a prostate cancer cell strain RNA (Ribonucleic Acid), a nanogold-labeled oligonucleotide probe and nitrocellulose, wherein the 11 genes are obtained by selection of the gene chip and verification of fluorescent quantitation polymerase chain reaction (PCR), have obvious expression difference in prostate cancer and benign prostatic hyperplasia and include IGF1, P27, AR, PSMA, KLK3, PCNA, Ki-67, KLK1, KLK2, TMPRSS2 and SREBF2. The nanogold-labeled chip for polygene diagnosis of prostate cancer can be applied in clinically early diagnosis on prostate cancer.

Description

technical field [0001] The invention relates to a gene chip, in particular to a nano-gold labeling chip for multigene diagnosis of prostate cancer. Background technique [0002] Nano-gold labeling chips have the advantages of simplicity, rapidity, high sensitivity, strong specificity, low cost, and less sample required, and can be used for qualitative and semi-quantitative detection. At present, nano-gold labeling chip technology has difficulties in combining nano-gold and probes, and it is difficult to grasp the concentration of nano-gold and the color development conditions. The development process of immunoassay and other rapid detection technologies using nano-gold as a marker requires huge investment and relatively long time. long time. [0003] In recent years, the incidence of male prostate cancer (PCa) in my country has shown an obvious upward trend. In European and American countries, prostate cancer has ranked first in the incidence of male malignant tumors for ma...

Claims

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 陈清标陈锡彬钟惟德
Owner 广州市第一人民医院
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