Non-contact gene amplification electrochemical quick detection method

Abstract

The invention relates to the field of biological engineering and particularly relates to a non-contact gene amplification electrochemical quick detection method. The method is used for monitoring a gene amplification process and animalizing the result of the gene amplification. According to the method, the change of ironic concentration in an amplification reaction solution is tested by a capacitively coupled contactless conductivity sensor; and the qualitative and quantitative information of a sample DNA or RNA can be provided cheaply, easily, quickly and automatically based on the reaction time.

Description

Technical field: [0001] The invention relates to the field of bioengineering, in particular to a non-contact type gene amplification electrochemical rapid detection method. Background technique: [0002] For gene amplification, whether the reaction product is specific and whether the result is accurate and reliable depends on strict analysis and identification techniques. At present, the detection / monitoring and analysis techniques of amplification products and amplification process can be divided into the following types: [0003] 1. Electrophoresis [see literature Ihira M, Yoshikawa T, Enomoto Y, Akimoto S, Ohashi M, Suga S, Nishimura N, Ozaki T, Nishiyama Y, Notomi T, Ohta Y, Asano Y: Rapid Diagnosis of Human Herpesvirus 6 Infection by a Novel DNA Amplification Method, Loop-Mediated Isothermal Amplification, J. Clin. Microbiol. 2004, 42(1), 140-145]. Including agarose gel electrophoresis, polyacrylamide gel electrophoresis and related enzyme digestion-gel electrophoresis. Thi...

AI Technical Summary

Problems solved by technology

Blot hybridization and dot hybridization based on visual hybridization results, although the method is simple and the equipment is cheap, they also have the same disadvantages as the electrophoresis method: the use of dyes, long cycle, risk of process contamination, poor quantitative effect, only endpoint analysis, etc.

The optical method effectively avoids the possibility of the product polluting the environment, making it possible to monitor the progress of the amplification reaction in real time and perform accurate quantitative analysis, but there are still disadvantages that cannot be ignored: there are strict requirements on the transparency of the relevant reagent solutions and containers; optical equipment is expensive ;Optical / electrical conversion device is required to output the results conveniently and automatically; the use of probes will significantly increase the operating cost

[0009] Although various current methods can complete the result analysis or process monitoring of the amplification reaction in a certain direction and within a certain range, they are not ideal in terms of low cost, high throughput, automation and miniaturization.

Images