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Method for efficiently inducing wheat-wheatgrass non-homologous chromosomes to translocate

A heterologous chromosome and wheat technology, which is applied in botany equipment and methods, application, plant gene improvement, etc., can solve the problem of low frequency of heterologous translocation chromosome induction, limitation of excellent gene transfer and utilization efficiency of wheat close relative wild plants, etc. problems, to achieve the effect of broadening the genetic base

Active Publication Date: 2013-02-06
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

In summary, the low frequency of induction of heterologous translocation chromosomes limits the transfer and utilization efficiency of excellent genes in wheat related wild plants, and it is urgent to establish a method for efficiently inducing heterologous chromosome translocation in wheat

Method used

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  • Method for efficiently inducing wheat-wheatgrass non-homologous chromosomes to translocate
  • Method for efficiently inducing wheat-wheatgrass non-homologous chromosomes to translocate
  • Method for efficiently inducing wheat-wheatgrass non-homologous chromosomes to translocate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Example 1. Preparation of Receptor Material for Inducing Wheat-Wheat Grass Heterologous Chromosomal Translocation

[0021]Seeds of wheat-wheatgrass addition line 4844-12 were sown in flower pots (30 cm in height, 24 cm in diameter) containing field soil, and 3 plants were planted in each pot after emergence. Under natural outdoor conditions and normal water and fertilizer management, positive Wheatgrass addition line 4844-12 plants including spikelets at the booting stage, spikelets that have not bloomed at the heading stage and / or florets that are opening, that is, the recipients for inducing wheat-Wheatgrass heterologous chromosome translocation body material.

Embodiment 2

[0022] Example 2, Inducing Wheat-Wheat Grass Heterologous Chromosomal Translocation

[0023] Take the wheat-wheatgrass obtained in step 1, including the spikelets at the booting stage (marked as A), the spikelets that have not bloomed at the heading stage (marked as B) and / or the florets that are opening (marked as C) A total of 75 plants of the additional line 4844-12 were divided into three batches (each batch was a repetition) and used 60 Co-γ rays were irradiated, the irradiation dose was 20Gy and the dose rate was 0.5Gy / min, and the above-mentioned irradiated receptor material (that is, the material used to obtain the wheat-wheatgrass heterologous chromosomal translocation line) Then move it back to outdoor natural conditions for normal water and fertilizer management, and harvest mature seeds according to the irradiation period (A, B and C).

[0024] Take 100 seeds at random from the seeds of the three treatments of A, B and C, place them in a petri dish after soaking, ...

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Abstract

The invention discloses a method for efficiently inducing wheat-wheatgrass non-homologous chromosomes to translocate. The method comprises the following step of irradiating wheat-wheatgrass non-homologous disome addition line plants and plants with blooming florets by means of 60 Co-gamma rays at 20 Gy of irradiation dose and 0.5 Gy / min of dose rate; and thus obtaining a wheat-wheatgrass non-homologous translocation line of which the translocation frequency is obviously improved (39.85 percent). In order to expand the genetic basis of wheat, the invention provides a high-efficiency method for transferring beneficial genes in wheatgrass chromosomes to the wheat, and the method has important significance in the aspects of basic theory research, actual application of breeding and the like.

Description

technical field [0001] The invention relates to a method for efficiently inducing heterologous chromosome translocation between wheat and wheatgrass. Background technique [0002] Wheat is the largest food crop in the world and the second largest food crop after rice in China, which plays an important role in food security. The increase of world population and the reduction of arable land have higher and higher requirements for wheat production. However, in recent years, the phylogenetic relationship of wheat varieties has become simplistic, and the genetic variation within the species has decreased sharply, resulting in less and less genetic resources within the cultivated varieties, and lower and lower ability to resist diseases and droughts. The narrow genetic basis has become the main obstacle of wheat breeding, and introducing foreign genes into wheat is the main method to broaden the genetic basis of wheat. Wild relatives of wheat are rich in genetic diversity and ha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
Inventor 刘伟华李立会宋利强江莉莉高爱农杨欣明张锦鹏李秀全鲁玉清
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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