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Method for producing monacolin k by fermenting monascus with solid matrix served as carrier

A matrix carrier, the technology of Monascus, applied in the field of fermentation production (Monacolin K) Monacolin K, which can solve the problems of low content and unstable content of Monacolin K

Inactive Publication Date: 2013-02-06
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This also makes the content of Monacolin K produced by the solid-state fermentation method with grain as the only raw material low and unstable

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0037] Bagasse is used as a solid matrix carrier, boiled in water for 20 minutes, particle size is 2mm, bagasse and nutrient solution are mixed at a mass ratio of 1:7, sterilized at 121°C for 20 minutes, and 15% (V / W) of Monascus 9901 seeds are added After mixing evenly, incubate at 30°C for 2 days, and at 28°C for 20 days, with stirring every 3 days. The composition of the nutrient solution is glucose 30g / L, soybean hydrolyzate 50mL / L, glycerin 400g / L, NaNO 3 10g / L, K 2 HPO 4 4g / L, MgSO 4 2g / L, ZnSO 4 ﹒ 7H 2 O 8g / L, corn steep liquor 50mL / L. After the fermentation, the samples were sterilized by high temperature and humidity, dried and pulverized, extracted with 70% alcohol in a water bath at 55°C for 1 hour, and determined by microfiltration to determine the content of MONACOLIN K. The content of MONACOLIN K is 11.31mg / g.

example 2

[0039] Mix corn cob and bagasse in a mass ratio of 3:2 as a solid matrix carrier, boil the carrier in water for 40 minutes, and the particle size is 2mm and 5mm respectively, mix the carrier and nutrient solution in a mass ratio of 1:9, sterilize at 121°C for 30 minutes, and insert 18% (V / W) Monascus 9901 seed liquid, mixed evenly, cultured at 28°C for 2 days, 25°C for 18 days, during which time it was stirred every 3 days. The composition of the nutrient solution is glucose 40g / L, soybean hydrolyzate 90mL / L, glycerin 350g / L, NaNO 3 8g / L, K 2 HPO 4 3g / L, MgSO 4 1.5g / L, ZnSO 4 ﹒ 7H 2O 6g / L, corn steep liquor 40mL / L. After the fermentation, the samples were sterilized by high temperature and humidity, dried and pulverized, extracted with 80% alcohol in a water bath at 55°C for 1.5 hours, and then microfiltration was used to determine the content of MONACOLIN K. The content of MONACOLIN K is 10.42mg / g.

example 3

[0041] Tea stalk powder is used as a solid matrix carrier, the carrier is boiled for 30 minutes, the particle size is 1mm, the carrier and nutrient solution are mixed at a mass ratio of 1:5, sterilized at 121°C for 25 minutes, and 13% (V / W) red yeast rice is added Bacteria 9901 seed solution, after mixing evenly, cultivate at 28°C for 2 days, and at 22°C for 25 days, during which time, stir once every 3 days. The composition of the nutrient solution is glucose 50g / L, soybean hydrolyzate 70mL / L, glycerin 400g / L, NaNO 3 5g / L, K 2 HPO 4 5g / L, MgSO 4 2.5g / L, ZnSO 4 ﹒ 7H 2 O 10g / L, corn steep liquor 30mL / L. After the fermentation, the samples were sterilized by high temperature and humidity, dried and pulverized, extracted with 90% alcohol in a 55°C water bath for 1.5 hours, and then microfiltration was used to determine the content of MONACOLIN K. The content of MONACOLIN K is 7.76mg / g.

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Abstract

The invention discloses a method for producing monacolin k by fermenting monascus with a solid matrix served as carrier. The method relates to the field of fermentation engineering. The method mainly comprises steps as follows: uniformly mixing a solid matrix carrier with nutrient liquid based on mass ratio of 1: (0.5 to 10); sterilizing for 20 to 60 minutes at 121 DEG C; cooling; then adding 10 to 20% (V / W) of monascus MGMCC0517 seed liquid; uniformly mixing; then standing to culture for 2 days at 28 to 30 DEG C; then culturing for 18 to 28 days at 20 to 28 DEG C, and synchronously agitating once every three ways; and drying and crushing a solid matrix material after fermenting, so as to obtain a monascus powder product of monacolin K. The method can be used for producing a biological activity material of monascus by a microbiological fermentation method by adopting various cheap solid matrixes as raw materials, so that high-value-added transformation can be achieved.

Description

technical field [0001] The invention relates to a method for producing physiologically active substances by solid-state fermentation using a solid substrate as a carrier, in particular a method for producing Monacolin K by fermentation. technical background [0002] Monaconlin K is a competitive inhibitor of the key enzyme hydroxymethylglutaryl coenzyme A reductase (HMG-CoA reductase) in human cholesterol biosynthesis, which can effectively reduce the level of low-density cholesterol (LDL) in the human body. [0003] At present, there are mainly solid-state fermentation and liquid-state fermentation methods for producing Monacolin K by fermentation of Monascus bacterium at home and abroad. [0004] The liquid fermentation method is carried out in a ventilated mechanical tank, and the medium can be artificially optimized. Our laboratory has reached a high level in the production of Monacolin K by using Monascus 9901 liquid fermentation glycerin. However, the investment in l...

Claims

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Application Information

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IPC IPC(8): C12P17/06C12R1/645
Inventor 许赣荣鲁利平张薄博
Owner JIANGNAN UNIV
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