Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for fermentation production of butyl alcohol by utilizing cellulose and hemicellulose in bamboos

A technology of hemicellulose and cellulose, applied in the field of bioenergy, can solve problems such as unseen butanol, and achieve the effect of cheap raw materials

Inactive Publication Date: 2013-02-13
SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI
View PDF3 Cites 2 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the use of bamboo to produce fuel ethanol has been reported, but there is no research on the use of bamboo to produce butanol

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for fermentation production of butyl alcohol by utilizing cellulose and hemicellulose in bamboos

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Bamboo (Ph. pubescens, Jiangxi Moso) was pulverized by ultrafine airflow, and 5g of bamboo powder was pulverized by ultrafine airflow to a particle size of 2000 mesh, and 50mL, 0.5% (w / w) sulfuric acid solution (solid-liquid ratio was added) 1:10), react at 160°C for 20 minutes, adjust the pH of the hydrolysate to 4.8, add 1.0IU / g xylanase substrate (purchased from Novozymes), cellulase 20FPU / g substrate (purchased from Novi The product, β-glucosidase 1CBU / g substrate (purchased from Novozymes), enzymatically digested at 50°C for 48h, and the shaker speed is 100r / min. Add 1% (w / v) activated carbon to 50℃ detoxify the enzymolysis solution for 1h, detoxify the speed at 100rpm, add 1.0% (w / v) corn steep liquor to the clear solution, connect with NCIMB8052 strain, anaerobic culture at 37℃ for 48h, fermentation product Among them, butanol is 9.86g / L, and the total solvent is 11.63g / L.

Embodiment 2

[0038] Weigh 5g of bamboo powder crushed by ultra-fine airflow to a particle size of 1500 mesh, add 40mL, 2% (w / w) sulfuric acid solution (solid-liquid ratio 1:8), react at 170°C for 10 minutes, adjust the pH of the hydrolysate 5.0, add 0.5IU / g substrate of xylanase, 30FPU / g substrate of cellulase, 10IU / g substrate of β-glucosidase, enzymolysis at 50℃ for 48h, and shaker speed 120r / min. Add 1.5% (w / v) activated carbon to detoxify the enzymolysis solution at 60°C for 0.5h, add 0.3%(w / v) ammonium sulfate to the clear solution, shake the rotation speed of 200rpm, connect to the NCIMB8052 strain, and culture at 35°C for 50h. The butanol in the fermentation product is 7.64 g / L, and the total solvent is 9.08 g / L.

Embodiment 3

[0040] Weigh 5g of bamboo powder that has been pulverized to a particle size of 100 mesh, add 60mL, 1.5% (w / w) sulfuric acid solution (solid-liquid ratio 1:12), react at 140°C for 30 minutes, adjust the pH of the hydrolyzate to 4.5, Add xylanase 0.1IU / g substrate, cellulase 30FPU / g substrate, β-glucosidase 10CBU / g substrate, enzymatically digest at 55°C for 45h, and shaker speed at 180r / min. Add 3% (w / v) activated carbon to detoxify the enzymatic hydrolysate at 40°C for 3 hours, add 0.1% (w / v) corn steep liquor to the clear solution, connect with NCIMB8052 strain, and culture at 38°C for 40 hours anaerobic. The fermented product contains 6.33g / L butanol and 8.06g / L total solvent.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Particle sizeaaaaaaaaaa
Login to View More

Abstract

The invention relates to the field of biological energy sources, in particular to a method for fermentation production of butyl alcohol by utilizing cellulose and hemicellulose in bamboos, which comprises the specific steps as follows: firstly, preprocessing; secondly, performing enzymolysis; thirdly, performing solid-liquid separation; fourthly, performing detoxification; fifthly, preparing a fermentation medium and culturing to obtain a fermentation product; and sixthly, rectifying the fermented product to obtain butyl alcohol. The method hydrolyzes rich cellulose and hemicellulose contained in bamboos to obtain a mixed carbon source containing glucose and xylose and is used for the growth and fermentation of biobutanol bacterial strains, so as to develop a new approach to the utilization of bamboo resources, realize the reuse of waste after processing the bamboos, and provide a cheaper raw material for the fermentation of butyl alcohol.

Description

Technical field [0001] The invention relates to the field of bioenergy, in particular to a method for fermenting butanol by using cellulose and hemicellulose in bamboo. Background technique [0002] my country is the country with the richest bamboo resources in the world. The sixth national forest resource inventory statistics show that the area of ​​bamboo forests in my country has exceeded 4.8 million hectares. 2 , Mainly distributed along the banks of the Yangtze River, with more than 500 species. According to the 2010-2015 analysis report on the development prospects of China’s bamboo industry, there will be 9 companies with annual sales revenue of 100 million yuan in bamboo products and 25 companies with more than 50 million yuan. The industry involves wood-based panels, pulp and paper, medicine, Bamboo charcoal, tourism and other industries. [0003] Butanol is not only a basic raw material for bulk chemical industry, but also a high-quality fuel and fuel additive. It is eas...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12P7/16
Inventor 沈兆兵刘莉史吉平林增祥张丽丽姜标
Owner SHANGHAI ADVANCED RES INST CHINESE ACADEMY OF SCI