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Method for rapidly detecting polysaccharide content of health enhancement oral liquid by adopting near infrared spectrum technology

A health-enhancing oral liquid and near-infrared spectroscopy technology, which is applied in the field of polysaccharide content detection, can solve the problems of long detection cycle, long time, unfavorable health-enhancing oral liquid, etc., and achieve the effect of high detection cost.

Inactive Publication Date: 2013-02-13
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, no matter what kind of chemical determination method, the sample needs to be pretreated, which consumes a large amount of chemical reagents and causes some pollution to the environment. However, due to the many pretreatment steps and long time, human error has a great impact on the final test results. , leading to large fluctuations in the determination of the polysaccharide content of the final product, and a long detection cycle (1-2 days), which is not conducive to the detection of Zengjian Oral Liquid products and the rapid quality control of the production process

Method used

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  • Method for rapidly detecting polysaccharide content of health enhancement oral liquid by adopting near infrared spectrum technology
  • Method for rapidly detecting polysaccharide content of health enhancement oral liquid by adopting near infrared spectrum technology
  • Method for rapidly detecting polysaccharide content of health enhancement oral liquid by adopting near infrared spectrum technology

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] The method for rapidly detecting the polysaccharide content of Zengjian Oral Liquid based on near-infrared spectroscopy provided by this embodiment may specifically include the following steps:

[0030] (1) Collect representative samples of different batches of Zengjian Oral Liquid produced normally

[0031] Different batches of Zengjian Oral Liquid samples were collected first, and the polysaccharide content was detected by conventional detection methods as reference data, and then the spectrum was scanned and collected by the Nicolet Antaris II near-infrared spectrometer from Thermo Fisher Scientific, analyzed and processed, and the model was established. Model Validation. The specific implementation is as follows:

[0032] (2) Determination of polysaccharide content in Zengjian Oral Liquid

[0033] 2.1 For the determination of polysaccharide content in Zengjian Oral Liquid during modeling, refer to "Determination of Reducing Sugar in Food"-GB / T5009.7-2008, the firs...

Embodiment 2

[0075] The method for rapidly detecting the polysaccharide content of Zengjian Oral Liquid based on near-infrared spectroscopy provided by this embodiment may specifically include the following steps:

[0076] (1) Collect 29 batches (pieces) of Zengjian Oral Liquid produced normally;

[0077] (2) The polysaccharide content of 29 batches of Zengjian oral samples was detected by routine laboratory testing methods as reference data. The specific testing method was the same as in Example 1, and the polysaccharide content range was 382.89-611.05 (mg / 100mL);

[0078] (3) 22 samples were randomly selected for modeling, and 7 samples were used as samples to be tested. The spectral data of 22 batches of samples were collected by Thermo Fisher Scientific near-infrared spectrometer NicoletAntaris II. For the original spectra, see figure 1 ;

[0079] (4) Partial least squares (PLS) was used to process the data, and the first-order differential and Norris smoothing preprocessing were perf...

Embodiment 3

[0092] The method for rapidly detecting the polysaccharide content of Zengjian Oral Liquid based on near-infrared spectroscopy provided by this embodiment may specifically include the following steps:

[0093] (1) Collect 40 batches of Zengjian Oral Liquid produced normally;

[0094] (2) For 40 batches of Zengjian oral samples, the polysaccharide content was detected by routine laboratory testing methods. As a reference data, the polysaccharide content range was: 572.61-684.51 (mg / 100mL);

[0095](3) 30 samples were randomly selected for modeling, and 10 samples were used as samples to be tested. The spectral data of 30 batches of samples were collected by Thermo Fisher Scientific near-infrared spectrometer Nicolet Antaris II. The original spectra obtained are shown in image 3 ;

[0096] (4) Partial least squares (PLS) was used to process the data, and the first-order differential and Norris smoothing preprocessing were performed on the modeled spectrum, and the collected sp...

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Abstract

The invention discloses a method for rapidly detecting the polysaccharide content of a health enhancement oral liquid by adopting a near infrared spectrum technology. The method comprises the following steps of: (1). collecting a representative health enhancement oral liquid sample; (2). detecting the polysaccharide content of the sample by adopting a conventional laboratorial detection method; (3). collecting near infrared spectrum data of the sample by adopting a near-infrared spectroscopy; (4). relating the collected spectrum data with the polysaccharide content, and establishing a quantitative model of the spectrum data and the polysaccharide content of the health enhancement oral liquid sample; and (5) collecting the near infrared spectrum data of a sample to be tested, and analyzing by utilizing the established quantitative model of the spectrum data and the polysaccharide content of the health enhancement oral liquid sample, so as to obtain the polysaccharide content of the sample to be tested. The method is stable, rapid, and low in cost, the detection period of the health enhancement oral liquid can be shortened, and support is provided for quality monitoring during the production process.

Description

technical field [0001] The invention belongs to the technical field of polysaccharide content detection, and in particular relates to a method for rapidly detecting the polysaccharide content of Zengjian Oral Liquid by near-infrared spectroscopy. Background technique [0002] Zengjian Oral Liquid is an immune modulator-compound polysaccharide extracted from a variety of natural plants by Infinitus (China) Co., Ltd. based on traditional Chinese medicine theory, using modern research results and practical experience, and then formulated with a variety of tonics and heat-clearing and detoxifying agents. , medicinal and edible natural plants, etc. are refined. Effectively regulate and enhance the body's immunity, improve the body's resistance, prevent the invasion of diseases, enhance and improve physical fitness. [0003] Polysaccharide content is the efficacy index of Zengjian Oral Liquid. There is no unified national standard for the determination method of polysaccharide co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/35G01N21/3577G01N21/359
Inventor 黄生权陆树杏
Owner INFINITUS (CHINA) CO LTD
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