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In-situ crosslinked hydrogel for intraocular tamponade, and preparation method and application thereof

An in-situ cross-linking, hydrogel technology, applied in medical science, prosthesis, etc., can solve the problem of lack of long-term intraocular fillers

Inactive Publication Date: 2013-03-06
PEOPLES HOSPITAL PEKING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, so far, there is a lack of ideal long-term intraocular fillers clinically.

Method used

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  • In-situ crosslinked hydrogel for intraocular tamponade, and preparation method and application thereof
  • In-situ crosslinked hydrogel for intraocular tamponade, and preparation method and application thereof
  • In-situ crosslinked hydrogel for intraocular tamponade, and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1, preparation of in situ cross-linked hydrogel

[0028] 4ARM-PEG-SH and PEG-O-EMA (n is 30) were dissolved in water respectively, and prepared into a solution with a concentration of 200 mg / mL, and the two solutions were mixed in vitro at a volume ratio of 1:1 (the In the system, the mass fraction ratio of 4ARM-PEG-SH to PEG-O-EMA was 1:1), and the in-situ cross-linking reaction was carried out at room temperature for 3 hours to obtain the in-situ cross-linked hydrogel.

Embodiment 2

[0029] Embodiment 2, preparation of in situ cross-linked hydrogel

[0030] 4ARM-PEG-SH and PEG-O-EMA (n is 60) were respectively dissolved in 0.9% sodium chloride aqueous solution by mass percentage to prepare a solution with a concentration of 50 mg / mL, and the solutions of the two Mix in vitro at a volume ratio of 1:10 (in this system, the mass fraction ratio of 4ARM-PEG-SH and PEG-O-EMA is 1:10), and carry out the in-situ cross-linking reaction for 3 h to obtain in-situ cross-linking Hydrogels.

Embodiment 3

[0031] Example 3. Preparation of in situ cross-linked hydrogel and its cell safety test

[0032] (1) Dissolve 4ARM-PEG-SH and PEG-O-EMA respectively in phosphate buffer solution with a concentration of 0.1mol / L at pH=7.4, and prepare a solution with a concentration of 50mg / mL. Mix in vitro at a volume ratio of 1:1, and perform in situ cross-linking reaction at room temperature for 3 hours to obtain gel blocks for cell experiments.

[0033] (2) The cell line (rat retinal pigment epithelial cell D407 cell line) was routinely cultured, and the culture medium was DMEM / F12=1:1 (purchased from Hyclone Company in the United States), adding fetal bovine serum (final concentration: 10%), and taking Cells passed down to passage 3-6 were used for experiments.

[0034] (3) The cells were digested with 0.25% trypsin, mixed evenly, and made into a cell suspension, which was then inoculated in a sterile 6-well culture plate at 5×106 / ml. Put in 37 degrees 5% CO 2 Cultivate in a constant te...

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Abstract

The invention discloses an in-situ crosslinked hydrogel for intraocular tamponade, and a preparation method and an application thereof. The preparation method comprises a step that the hydrogel is obtained through an in-situ crosslinking reaction of 4arm-PEG-thiol having a structure represented by formula (I) shown in the specification and a polymer having a structure represented by formula (II). The invention also provides an application of the in-situ crosslinked hydrogen prepared through the method in the preparation of artificial vitreous bodies. Compared with traditional artificial vitreous body substitute materials, the in-situ crosslinked hydrogel which is the artificial vitreous body substitute material provided by the invention has the advantages of long-term intraocular tamponade, and no obvious toxic reactions, stimulates an original natural vitreous body structure, and is hydrophilic.

Description

technical field [0001] The invention relates to an in-situ cross-linked hydrogel for intraocular filling, a preparation method and application thereof. Background technique [0002] Vitrectomy is a commonly used clinical surgical method for the treatment of retinal detachment, vitreous hemorrhage, trauma to the posterior segment of the eye and other fundus diseases. After the vitreous body is removed, intraocular fillers need to be injected to reset the pressure on the retina and maintain the shape of the eyeball. Commonly used intraocular fillers include silicone oil and inert gas (such as SF 6 、C 2 f 6 and C 3 f 8 Wait). However, silicone oil filling has complications such as silicone oil emulsification, secondary glaucoma, corneal band degeneration, and concurrent cataract, which must be removed by a second operation. There is nothing that can be done for those whose eyes are dependent on silicone oil and the eyeballs shrink after the silicone oil is taken out. Th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08J3/24C08J3/075C08L71/00A61L27/18A61L27/52
Inventor 姜燕荣陶勇张燕郭宝华黄延宾童新明
Owner PEOPLES HOSPITAL PEKING UNIV
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