Co-culture support of multiple types of cells

Abstract

The invention relates to a co-culture support of multiple types of cells. The support comprises a support surface and a bracing frame connected with the support surface, and the support surface is provided with fixing orifices. The edge of the support surface is connected with the bracing frame. The support can realize the co-culture of 3-5 types of cells as needed, and the support is suitable for the co-culture of multiple types of adherent cells, and suspension or semi-suspension cells. Various cells respectively grow in different small cell culture inserts or dishes, the pollution and the mixing of different types of cells are not caused, and the cells are easy to gain.

Description

Technical field [0001] The invention relates to a culture scaffold, in particular to a multi-cell co-culture scaffold. Background technique [0002] Cell culture is the growth and reproduction of cells under in vitro conditions. It has the advantages of directly observing the morphology and life activities of living cells, directly observing cell changes, easy to provide a large number of experimental objects with similar biological characteristics, and low cost. The in vitro culture of cells can be a single type of cell culture and multiple types of cell culture together. [0003] A single cell culture, which is currently the most widely used method in basic medicine and the combination of basic and clinical research. It is mainly used to study the morphology, gene and protein expression of a certain cell, and in different processing factors. Changes under the action and its mechanism. Although the cells live independently in the artificially simulated in vivo environment duri...

AI Technical Summary

Problems solved by technology

But this product has the following disadvantages: 1) There are 6 small holes in a large co-culture well of the culture plate, the height of each small hole is only 0.8 mm, and the distance between each small hole is too close, so when operating If there is a slight shaking, the cells in one small hole may move to another small hole, causing contamination and mixing between different cells; and because the shaking is not possible, the cells will adhere unevenly, and there will be many central cells. There are few surrounding cells, which will affect the growth of cells; and it is also difficult to operate when harvesting cells; 2) Because the growth area of ​​the small holes is limited (each small hole can only hold a maximum of 300 microliters of liquid), it is not suitable for Experiments that require a large number of cells; 3) only suitable for adherent cells, not suitable for the culture of suspension and semi-suspension cells

[0005] In summary, the existing system IdMOC that can co-culture a variety of cells TM It has the following deficiencies: 1) It is easy to cause contamination and mixing between different cells; 2) Cells adhere to the wall unevenly; 3) It is not suitable for experiments that require a large number of cells; 4) It is only suitable for adherent cells, not suitable for suspension and semi-suspension cells 5) It is difficult to harvest cells

In addition, IdMOC TM The system is mainly used to evaluate the susceptibility and toxic effects of poisons on various organ cells, and it is difficult to meet the different needs of researchers for the co-culture of various cells

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