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Lactobacillus bulgaricus enrichment culture medium and preparation method thereof

A technology of enrichment culture medium and lactobacillus, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of unfavorable industrial production, expensive culture medium, cumbersome production, etc., and achieve easy preparation, The ingredients are simple and the ingredients are cheap

Active Publication Date: 2013-03-06
SHAANXI YATAI DAIRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this culture medium is expensive, the composition is complex, and the production is cumbersome, which is not conducive to industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Lactobacillus bulgaricus enrichment medium composition: lactose 0.4g, newborn bovine serum 0.4mL, casein hydrolyzate 0.8g; 0.09mL of 1% hydroxyproline; 0.05mL of 1% glutamic acid; K 2 HPO 4 0.59g, water 100mL, pH value 6.5.

[0024] Among them, casein hydrolyzate is commercially available from Rosenbo Technology Co., Ltd., and is a yellow powder, easily soluble in water.

[0025] Its preparation method is:

[0026] 1) Preparation of concentrated solution of hydroxyproline and glutamic acid:

[0027] Hydroxyproline and glutamic acid were formulated into a 1% concentrated solution, and then filtered through a 0.2-micron membrane that was sterilized at 121°C for 20 minutes.

[0028] 2) Preparation of newborn bovine serum:

[0029] Newborn bovine serum was filtered through a 0.2 micron membrane that was sterilized at 121°C for 20 minutes.

[0030] 3) Preparation of Lactobacillus bulgaricus enrichment medium:

[0031] Lactose 0.4g, casein hydrolyzate 0.8g, K 2 HPO 4 ...

Embodiment 2

[0034] Lactobacillus bulgaricus enrichment medium composition: 0.4g lactose, 0.4mL newborn bovine serum, 1g casein hydrolyzate; 0.10mL hydroxyproline with a mass concentration of 1%; 0.04mL glutamic acid with a mass concentration of 1%; K 2 HPO 4 0.59g, water 100mL, pH value 6.6.

[0035] Its preparation method is:

[0036] 1) Preparation of concentrated solution of hydroxyproline and glutamic acid:

[0037] Hydroxyproline and glutamic acid were formulated into a 1% concentrated solution, and then filtered through a 0.2-micron membrane that was sterilized at 121°C for 20 minutes.

[0038] 2) Preparation of newborn bovine serum:

[0039] Newborn bovine serum was filtered through a 0.2 micron membrane that was sterilized at 121°C for 20 minutes.

[0040] 3) Preparation of Lactobacillus bulgaricus enrichment medium:

[0041] Lactose 0.4g, casein hydrolyzate 1g, K 2 HPO 4 0.59g, mix and dissolve in water, adjust the pH to 6.6 with 1moL / L HCl solution, then sterilize at 118°...

Embodiment 3

[0044] Lactobacillus bulgaricus enrichment medium composition: 0.4g lactose, 0.3mL newborn bovine serum, 0.8g casein hydrolyzate; 0.12mL hydroxyproline with a mass concentration of 1%; 0.05mL glutamic acid with a mass concentration of 1%; K 2 HPO 40.59g, water 100mL, pH value 6.8.

[0045] Its preparation method is:

[0046] 1) Preparation of concentrated solution of hydroxyproline and glutamic acid:

[0047] Hydroxyproline and glutamic acid were formulated into a 1% concentrated solution, and then filtered through a 0.2-micron membrane that was sterilized at 121°C for 20 minutes.

[0048] 2) Preparation of newborn bovine serum:

[0049] Newborn bovine serum was filtered through a 0.2 micron membrane that was sterilized at 121°C for 20 minutes.

[0050] 3) Preparation of Lactobacillus bulgaricus enrichment medium:

[0051] Lactose 0.4g, casein hydrolyzate 0.8g, K 2 HPO 4 0.59g, mix and dissolve in water, adjust the pH to 6.8 with 1moL / L HCl solution, then sterilize at 1...

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Abstract

The invention discloses a lactobacillus bulgaricus enrichment culture medium and a preparation method thereof. The lactobacillus bulgaricus enrichment culture medium comprises 0.4 g of lactose, 0.4 mL of new-born calf serum, 0.8 g of casein hydrolysate, 0.090 mL of hydroxyproline with mass concentration of 1 percent, 0.050 mL of glutamic acid with mass concentration of 1 percent and 0.59 g of K2HPO4 in every 100 ml of water, wherein the pH (Potential of Hydrogen) value is 6.5; and the casein hydrolysate is purchased from Rosenbo Science and Technology Limited. The culture medium is a cheap culture medium capable of realizing large-scale growth of lactobacillus bulgaricus. The culture medium has the advantages of simple and cheap ingredients which are all more common carbon and nitrogen sources and easiness in preparation, so that the method is a good method for industrial production of lactic acid bacteria.

Description

technical field [0001] The invention belongs to the technical field of probiotic product preparation, and relates to a probiotic culture medium, in particular to a Lactobacillus bulgaricus enrichment culture medium and a preparation method thereof. Background technique [0002] In recent years, with the development of yogurt production technology, the preparation method of lactic acid bacteria starter has also been improved accordingly. A key factor that determines the quality of yogurt is the starter, and most domestic yogurt manufacturers use traditional liquid starter. However, the traditional liquid fermentation agent has a long period of existence, a large amount of inoculum, cumbersome operation, and a low number of viable bacteria (10 7 -10 8 cfu / mL); strains are prone to contamination, degradation, variation and many other disadvantages, so the direct input freeze-dried starter has become the main direction for the development of yogurt starter. [0003] Lactobaci...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/225
Inventor 陈合李串娜舒国伟王勇
Owner SHAANXI YATAI DAIRY CO LTD
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