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Lactobacillus bulgaricus enrichment culture medium and preparation method thereof

A technology of enrichment culture medium and lactobacillus, applied in the direction of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of expensive medium, unfavorable industrial production, complex components, etc., and achieve easy preparation, The ingredients are cheap, the ingredients are simple and the effect

Active Publication Date: 2014-06-04
SHAANXI YATAI DAIRY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But this culture medium is expensive, the composition is complex, and the production is cumbersome, which is not conducive to industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Lactobacillus bulgaricus enrichment medium composition: lactose 0.4g, newborn calf serum 0.4mL, casein hydrolysate 0.8g; mass concentration of 1% hydroxyproline 0.09mL; mass concentration of 1% glutamic acid 0.05mL; K 2 HPO 4 0.59g, 100mL water, pH 6.5.

[0024] Among them, the casein hydrolysate is commercially available from Rosenbau Technology Co., Ltd., is a yellow powder and is easily soluble in water.

[0025] The preparation method is:

[0026] 1) Preparation of concentrated solution of hydroxyproline and glutamic acid:

[0027] The hydroxyproline and glutamic acid were prepared into a 1% concentrated solution, and then filtered through a 0.2 micron membrane sterilized at 121°C for 20 min.

[0028] 2) Preparation of newborn calf serum:

[0029] The newborn calf serum was filtered through a 0.2 micron membrane sterilized at 121°C for 20 min.

[0030] 3) Preparation of Enrichment Medium for Lactobacillus bulgaricus:

[0031] Add 0.4g lactose, 0.8g casein hydrolysate, K 2 HPO 4 ...

Embodiment 2

[0034] Lactobacillus bulgaricus enrichment medium composition: lactose 0.4g, newborn calf serum 0.4mL, casein hydrolysate 1g; mass concentration of 1% hydroxyproline 0.10mL; mass concentration of 1% glutamic acid 0.04mL; K 2 HPO 4 0.59g, 100mL water, pH 6.6.

[0035] The preparation method is:

[0036] 1) Preparation of concentrated solution of hydroxyproline and glutamic acid:

[0037] The hydroxyproline and glutamic acid were prepared into a 1% concentrated solution, and then filtered through a 0.2 micron membrane sterilized at 121°C for 20 min.

[0038] 2) Preparation of newborn calf serum:

[0039] The newborn calf serum was filtered through a 0.2 micron membrane sterilized at 121°C for 20 min.

[0040] 3) Preparation of Enrichment Medium for Lactobacillus bulgaricus:

[0041] Add 0.4g lactose, 1g casein hydrolysate, K 2 HPO 4 0.59g, mixed and dissolved in water, adjusted pH to 6.6 with 1moL / L HCl solution, then sterilized at 118°C for 15min, and finally added 0.4mL of membrane-filter...

Embodiment 3

[0044] Lactobacillus bulgaricus enrichment medium composition: lactose 0.4g, newborn calf serum 0.3mL, casein hydrolysate 0.8g; mass concentration of 1% hydroxyproline 0.12mL; mass concentration of 1% glutamic acid 0.05mL; K 2 HPO 4 0.59g, 100mL water, pH 6.8.

[0045] The preparation method is:

[0046] 1) Preparation of concentrated solution of hydroxyproline and glutamic acid:

[0047] The hydroxyproline and glutamic acid were prepared into a 1% concentrated solution, and then filtered through a 0.2 micron membrane sterilized at 121°C for 20 min.

[0048] 2) Preparation of newborn calf serum:

[0049] The newborn calf serum was filtered through a 0.2 micron membrane sterilized at 121°C for 20 min.

[0050] 3) Preparation of Enrichment Medium for Lactobacillus bulgaricus:

[0051] Add 0.4g lactose, 0.8g casein hydrolysate, K 2 HPO 4 0.59g, mixed and dissolved in water, adjusted pH to 6.8 with 1moL / L HCl solution, then sterilized at 118°C for 15 minutes, and finally added 0.3mL of membra...

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Abstract

The invention discloses a lactobacillus bulgaricus enrichment culture medium and a preparation method thereof. The lactobacillus bulgaricus enrichment culture medium comprises 0.4 g of lactose, 0.4 mL of new-born calf serum, 0.8 g of casein hydrolysate, 0.090 mL of hydroxyproline with mass concentration of 1 percent, 0.050 mL of glutamic acid with mass concentration of 1 percent and 0.59 g of K2HPO4 in every 100 ml of water, wherein the pH (Potential of Hydrogen) value is 6.5; and the casein hydrolysate is purchased from Rosenbo Science and Technology Limited. The culture medium is a cheap culture medium capable of realizing large-scale growth of lactobacillus bulgaricus. The culture medium has the advantages of simple and cheap ingredients which are all more common carbon and nitrogen sources and easiness in preparation, so that the method is a good method for industrial production of lactic acid bacteria.

Description

Technical field [0001] The invention belongs to the technical field of preparation of probiotic products, and relates to a probiotic culture medium, in particular to a lactobacillus bulgaricus culture medium and a preparation method thereof. Background technique [0002] In recent years, with the development of yogurt production technology, the preparation method of lactic acid bacteria starter has been correspondingly improved. A key factor that determines the quality of yogurt is starter. Most domestic yogurt manufacturers use traditional liquid starter. However, the traditional liquid starter has a long cycle, a large amount of inoculation, cumbersome operation, and low number of viable bacteria (10 7 -10 8 cfu / mL); bacterial strains are prone to contamination, degradation, mutation and many other disadvantages, so direct injection freeze-dried starter has become the main direction of the development of yogurt starter. [0003] Lactobacillus bulgaricus and Streptococcus thermop...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12R1/225
Inventor 陈合李串娜舒国伟王勇
Owner SHAANXI YATAI DAIRY CO LTD
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