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Microcystis culture medium formula
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A culture medium formula and culture medium technology, applied in the direction of microorganism-based methods, microorganisms, single-cell algae, etc., to achieve the effect of fast growth rate and high survival rate
Inactive Publication Date: 2013-03-20
溧阳市天目湖保健品有限公司
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Problems solved by technology
However, there are few reports on the culture method of Microcystis at present, and there are even fewer reports on the culture medium specially used for Microcystis
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Embodiment 1
[0011] Prepare the culture medium with the following formula:
[0012] NaNO 3 1g / L, NH 4 NO 3 100mg / L, KNO 3 450mg / L, CaCl 2 .2H 2 O 500mg / L, MgSO 4 .7H 2 O 170mg / L, K 2 HPO 4 200mg / L, KI 0.5mg / L, H 3 BO 3 9.1mg / L, MnSO 4 .4H 2 O 20mg / L, ZnSO 4 .7H 2 O 5.3mg / L, Mo(NO 3 ) 3 .5H 2 O2mg / LCuSO 4 .5H 2 O 0.02mg / L, CoCl 2 .6H 2 O 0.02mg / L;
[0013] Ferric citrate 6mg / L, Na 2 -EDTA.2H 2 O 15mg / L, inositol 15mg / L, niacin 0.2mg / L, vitamin B 12 0.7mg / L, NNA 0.1mg / L, IBA 0.05mg / L.
[0014] Microcystis aeruginosa in the logarithmic growth phase was inoculated into the medium for cultivation, with light for 8 hours a day, the temperature of the incubator was controlled at about 25 degrees Celsius, and the cell density of Microcystis in the medium was recorded every 3 days.
Embodiment 2
[0016] NaNO 3 1.2g / L, NH 4 NO 3 105mg / L, KNO 3 455mg / L, CaCl 2 .2H 2 O 510mg / L, MgSO 4 .7H 2 O 175mg / L, K 2 HPO 4 205mg / L, KI 0.55mg / L, H 3 BO 3 9.3mg / L, MnSO 4 .4H 2 O 20.5mg / L, ZnSO 4 .7H 2 O 5.5mg / L, Mo(NO 3 ) 3 .5H 2 O 3mg / L, CuSO 4 .5H 2 O 0.03mg / L, CoCl 2 .6H 2 O 0.03mg / L;
[0017] Ferric citrate 10mg / L, Na 2 -EDTA.2H 2 O 20mg / L, inositol 20mg / L, niacin 0.3mg / L, vitamin B 12 0.8mg / L, NNA 0.15mg / L, IBA 0.08mg / L.
[0018] Microcystis aeruginosa in the logarithmic growth phase was inoculated into the medium for cultivation, with light for 8 hours a day, the temperature of the incubator was controlled at about 25 degrees Celsius, and the cell density of Microcystis in the medium was recorded every 3 days.
Embodiment 3
[0020] NaNO 3 1.1g / L, NH 4 NO 3 105mg / L, KNO 3 455mg / L, CaCl 2 .2H 2 O 510mg / L, MgSO 4 .7H 2 O 175mg / L, K 2 HPO 4 205mg / L, KI 0.55mg / L, H 3 BO 3 9.3mg / L, MnSO 4 .4H 2 O 20.5mg / L, ZnSO 4 .7H 2 O 5.5mg / L, Mo(NO 3 ) 3 .5H 2 O 2.5mg / L, CuSO 4 .5H 2 O 0.025mg / L, CoCl 2 .6H 2 O 0.025mg / L;
[0021] Ferric citrate 8mg / L, Na 2 -EDTA.2H 2 O 18mg / L, inositol 18mg / L, niacin 0.25mg / L, vitamin B 12 0.75mg / L, NNA 0.12mg / L, IBA 0.06mg / L.
[0022] Microcystis aeruginosa in the logarithmic growth phase was inoculated into the medium for cultivation, with light for 8 hours a day, the temperature of the incubator was controlled at about 25 degrees Celsius, and the cell density of Microcystis in the medium was recorded every 3 days.
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Abstract
The invention provides a microcystis culture medium. The formula of the microcystis culture medium comprises 1-1.2g / L of NaNO3, 100-105mg / L of NH4NO3, 450-455mg / L of KNO3, 500-510mg / L of CaCl2.2H2O, 170-175mg / L of MgSO4.7H2O, 200-205mg / L of K2HPO4, 0.5-0.55mg / L of KI, 9.1-9.3mg / L of H3BO3, 20-20.5mg / L of MnSO4.4H2O, 5.3-5.5mg / L of ZnSO4.7H2O, 0.02-0.03mg / L of CuSO4.5H2O, 0.02-0.03mg / L of CoCl2.6H2O, 6-10mg / L of ferric citrate, 15-20mg / L of Na2-EDTA, 15-20mg / L of inositol, 0.2-0.3mg / L of nicotinic acid, 0.7-0.8mg / L of vitamin B12, 0.1-0.15mg / L of NNA and 0.05-0.08mg / L of IBA. The microcystis culture medium has the characteristics of high survival rate and fast growth rate when microcystis is cultured by the medium, and cannot be polluted by other bacteria.
Description
technical field [0001] The invention relates to an algae culture medium, in particular to a culture medium formula of Microcystis. Background technique [0002] Microcystins (MCs) are a cyclic heptapeptide compound whose basic structure is a ring (D-alanine-L-X-D-erythro-methyl-β-D-isoaspartic acid-L-Z-Adda-D- isoglutamic acid-N-methyl dehydroalanine), in which N-methyl dehydroalanine is a special amino acid containing α, β unsaturated double bonds, Adda structure is 3-amino-9 -Methoxy-2,6,8-trimethyl-10-phenyl-4,6-dienoic acid. X and Y are two variable L amino acids respectively. The amino acid replacement or the demethylation of other amino acids can lead to many types of toxins, among which MC-LR (molecular formula: C49H75N13O12) is the most toxic. [0003] In recent years, my country's Taihu Lake, Songhua River, Huaihe River and other drinking water sources have been severely polluted by cyanobacteria blooms, releasing a large amount of MC-LR into the water body. The h...
Claims
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