Preparation method of raspberry fermenting substance for cigarette
A technology of raspberry and fermented products, which is applied in the field of preparation of raspberry fermented products for tobacco, can solve the problems of changing the characteristic aroma of raspberry spices, unstable quality, turbidity, etc. gas effect
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[0022] Example 1
[0023] 1) Medium preparation
[0024] a. Raw material pretreatment: extract raspberries and water at a weight ratio of 1:5 and reflux for 3 hours, place the extract for 24 hours and filter, and collect the filtrate to obtain a raspberry extract;
[0025] b. Add glucose: add 0.2% glucose by weight to the prepared raspberry extract to prepare a culture medium;
[0026] 2) Sterilization of culture medium
[0027] Sterilize the medium at 115°C for 20 minutes;
[0028] 3) Strain preparation
[0029] The active dry acetic acid strain is added to 10 times the mass of normal saline, and filtered with filter paper under aseptic conditions, and the filtrate is taken as the strain.
[0030] 4) Vaccination
[0031] Under aseptic conditions, inoculate the bacteria in the culture medium with an inoculum amount of 0.05%;
[0032] 5) Fermentation
[0033] The pH value was 6, the stirring speed was 50 rpm, and the anaerobic fermentation was carried out at 25°C for 2 days, and then at 4°C fo...
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[0037] Example 2
[0038] 1) Medium preparation
[0039] a. Raw material pretreatment: extract raspberries and water at a weight ratio of 1:10 for 3 hours at reflux, leave the extract for 24 hours and then filter, and collect the filtrate to obtain a raspberry extract;
[0040] b. Add glucose: add 0.8% glucose by weight to the prepared raspberry extract to prepare a culture medium;
[0041] 2) Sterilization of culture medium
[0042] Sterilize the medium at 115°C for 20 minutes;
[0043] 3) Strain preparation
[0044] The active dry acetic acid strain is added to 10 times the mass of normal saline, and filtered with filter paper under aseptic conditions, and the filtrate is taken as the strain.
[0045] 4) Vaccination
[0046] Under aseptic conditions, inoculate the bacteria in the culture medium with an inoculum amount of 0.1%;
[0047] 5) Fermentation
[0048] The pH value was 7, the stirring speed was 100 rpm, and the anaerobic fermentation was carried out at 36°C for 4 days, and then at 4...
Example Embodiment
[0052] Example 3
[0053] 1) Medium preparation
[0054] a. Pretreatment of raw materials: reflux the raspberry and water at a weight ratio of 1:15 for 3 hours, place the extract for 36 hours and filter, and collect the filtrate to obtain the raspberry extract;
[0055] b. Add glucose: add 2% glucose by weight to the prepared raspberry extract to prepare a culture medium;
[0056] 2) Sterilization of culture medium
[0057] Sterilize the medium at 115°C for 20 minutes;
[0058] 3) Strain preparation
[0059] The active dry acetic acid strain is added to 10 times the mass of normal saline, and filtered with filter paper under aseptic conditions, and the filtrate is taken as the strain.
[0060] 4) Vaccination
[0061] Under aseptic conditions, inoculate the strains in the culture medium with an inoculum amount of 0.15%;
[0062] 5) Fermentation
[0063] At pH 7, the stirring speed was 100 rpm, anaerobic fermentation was carried out at 36°C for 6 days, and then at 4°C for 8 days.
[0064] 6) Qua...
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