Method for determining significant correlation between physicochemical property of rhizosphere soil of S-amino-acid-rich transgenic soybeans and diversity of microbial communities

Abstract

The invention discloses a method for determining a significant correlation between a physicochemical property of rhizosphere soil of S-amino-acid-rich transgenic soybeans and a diversity of microbial communities, belonging to the technical field of soil microbe research and relating to a technology for determining a positive and negative correlated significance between the physicochemical property of the rhizosphere soil of the transgenic soybeans and the diversity of the microbial communities. The invention aims to achieve the effect that the positive and negative correlated significance between the physicochemical property of the rhizosphere soil of the S-amino-acid-rich transgenic soybeans and diversity indexes of the microbial communities can be determined respectively by a scientific method. Key points of the technical scheme for solving the problem are as follows: according to different diversity indexes, utilizing IBM SPSS Statistics 19 to measure a pearson correlation coefficient among measurement results of microbial community structures of the rhizosphere soil of the transgenic soybeans with heredity and functional diversity, pH values of the corresponding soil, carbon nitrogen ratios and sulfur contents to display correlated significant levels; and finally determining sensitive significance of the diversity of the microbial communities of the soil on the physicochemical property of the soil. The method can be mainly used for determining the sensitive significance between the diversity of the microbial communities of the rhizosphere soil of other transgenic soybeans and the physicochemical property of the corresponding soil.

Description

1: Technical field [0001] The invention belongs to the technical field of soil microbiology research, and specifically relates to a technology for determining the sensitivity and significance of the microbial community diversity index of transgenic soybean rhizosphere soil to relevant soil physical and chemical properties under field planting mode. Two: background technology [0002] At present, the research methods of soil microbial community diversity mainly include PLFA microbial lipid analysis method, BIOLOG microplate method, and DNA fingerprint analysis method based on PCR-amplified rRNA, etc. Scientists usually use the change of PLFA type and quantity to explore the change of soil microbial community structure diversity, and use PCR-DGGE analysis to display the amplification of soil bacterial 16SrDNA and the increase and decrease of bacterial species to explore the genetic diversity of soil microorganisms. To explore the soil culturable microbial community (including ...

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Problems solved by technology

Although the above literatures are concerned with the effects of S-amino acid-rich transgenic soybean on the content of organic elements in rhizosphere soil, soil microbial community structure and functional diversity, the physical and chemical properties of S-amino acid-rich transgenic soybean rhizosphere soil (such as pH value, carbon Nitrogen ratio and sulfur content) to the sensitivity significance of the soil microbial community to the structural, genetic and functional diversity indices represented by the results of PLFA, PCR-DGGE and BIOLOG (-GN, -GP, -FF and -ECO) Research methods and results have not been reported so far

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