Probiotics viable bacteria powder produced by whole oat solid-state mixed fermentation and preparation method of probiotics viable bacteria powder
A technology of solid-state fermentation and combined live bacteria powder, which is applied in powder delivery, pharmaceutical formulations, and medical raw materials derived from bacteria, etc. It can solve problems such as unsuitable for long-term consumption, and achieve the effect of improving intestinal health and regulating intestinal health
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example 1
[0033] Oat solid-state fermentation medium (%): whole oat powder 55; water 45; sterilized at 115°C for 20 minutes.
[0034] Inoculation and culture conditions: first inoculate Lactobacillus plantarum P-8 (L.plantarum P-8) and Lactobacillus casei Zhang (L.casei Zhang) for direct injection, and then inoculate Bifidobacterium lactis V9 (B. lactis V9) direct injection, and mixed bacteria fermentation for 16 hours, the inoculation amount of the three strains of lactic acid bacteria direct injection is 5% (v / w), the pH is natural, the culture temperature is 37±1°C, and the total culture is 24 hours. For fresh koji The number of viable bacteria in the probiotics was detected.
[0035] The total number of live bacteria measured was 3.56×10 9 CFU / g. Among them, the number of live bacteria of Lactobacillus plantarum P-8 (L.plantarum P-8) is 1.82×10 9 CFU / g, the viable count of Lactobacillus casei Zhang (L.casei Zhang) was 1.74×10 9 CFU / g, the number of live bacteria of Bifidobacteri...
example 2
[0037] Oat solid-state fermentation medium (%): oat whole powder 50; water 50; sterilized at 115°C for 20 minutes.
[0038] Inoculation and culture conditions: first inoculate Lactobacillus plantarum P-8 (L.plantarum P-8) and Lactobacillus casei Zhang (L.casei Zhang) for direct injection, and then inoculate Bifidobacterium lactis V9 (B. lactis V9) direct injection, and mixed bacteria fermentation for 16 hours, the inoculation amount of the three strains of lactic acid bacteria direct injection is 5% (v / w), the pH is natural, the culture temperature is 37±1°C, and the total culture is 24 hours. For fresh koji The number of viable bacteria in the probiotics was detected.
[0039] The total number of live bacteria measured was 5.59×10 9 CFU / g. Among them, the number of live bacteria of Lactobacillus plantarum P-8 (L.plantarum P-8) is 2.57×10 9 CFU / g, the viable count of Lactobacillus casei Zhang (L.casei Zhang) is 3.21×10 9 CFU / g, the number of live bacteria of Bifidobacteriu...
example 3
[0041] Oat solid-state fermentation medium (%): whole oat powder 45%; water 55%; sterilized at 115°C for 20min.
[0042] Inoculation and culture conditions: first inoculate Lactobacillus plantarum P-8 (L.plantarum P-8) and Lactobacillus casei Zhang (L.casei Zhang) for direct injection, and then inoculate Bifidobacterium lactis V9 (B. lactis V9) direct injection, and mixed bacteria fermentation for 16 hours, the inoculation amount of the three strains of lactic acid bacteria direct injection is 5% (v / w), the pH is natural, the culture temperature is 37±1°C, and the total culture is 24 hours. For fresh koji The number of viable bacteria in the probiotics was detected.
[0043] The total number of live bacteria measured was 3.69×10 9 CFU / g. Among them, the number of live bacteria of Lactobacillus plantarum P-8 (L.plantarum P-8) is 1.60×10 9 CFU / g, the viable count of Lactobacillus casei Zhang (L.casei Zhang) is 2.08×10 9 CFU / g, the number of live bacteria of Bifidobacterium l...
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