Method for making tissue chip based on one-step molding

Abstract

The invention provides a method for making a tissue chip based on one-step molding. The method is characterized in that the tissue chip is made through one-step molding without preparing a donor which is paraffin or an acceptor which is a paraffin tissue block. The method concretely comprises the following steps: 1, taking a mold frame, putting a tissue in the embedding tube of the mold frame in a required size and a required tangent plane, dumping paraffin for embedding, and allowing the paraffin to stand for a period of time to carry out polymerization molding of the paraffin; and 2, taking a paraffin-embedded frame, placing the mold frame having the embedding structure obtained in step 1 in the embedding frame on flat glass, dumping molten paraffin into the paraffin-embedded frame, allowing the paraffin in the porous tubes of the mold frame and the paraffin in the embedded frame to melt, and removing the embedded frame after the melting to complete the tissue embedding. According to the invention, the tissue positions and the embedding accuracy are strictly controlled, and no burrs or discontinuous cracks appear during punching, so the method has the advantages of cheapness, convenience, high efficiency, time saving and labor saving, and can be popularized to each pathology department and relevant research units.

Description

technical field

[0001] The invention relates to a method for making a tissue chip based on one-shot molding, which is used for preparing a tissue chip and belongs to the technical field of tissue chips. Background technique

[0002] Tissue chip (tissue chip) is an important branch of biochip technology, which can arrange dozens or even thousands of clinical tissue specimens of different individuals on a slide for analysis and research in a pre-designed order, and conduct the original analysis of the same index. Bit histological research is a high-throughput, multi-sample analytical tool. Tissue chip technology can analyze hundreds or even thousands of tissue samples at the same time, and conduct research on the expression of one or more specific proteins. Since the technology came out in 1998, it has been popularized and applied in a wide range due to its large-scale, high-throughput, and standardization advantages. Tissue chips together with gene chips and protein chips c...

Examples

Embodiment Construction

[0016] The present invention will be described in detail below in conjunction with the examples.

[0017] 1. Prepare the mold frame and embedding frame for making tissue chips:

[0018] Such as figure 1 Shown is a schematic diagram of the tissue chip casting mold frame, which is composed of a chip array orifice plate and an embedding tube. There are supports around the mold frame, and the height of the position is controlled by nuts. The embedding frame of the tissue chip is composed of two vertically bent copper strips, with a length of 70mm, a width of 40mm, and a height of 20mm, which can be freely separated and assembled into rectangles with different internal diameters.

[0019] 2. Tissue sampling:

[0020] According to the tissue to be prepared, find out and mark the target tissue area. According to the size of the target area, select a flat knife with an appropriate width, and cut vertically downward along the marked line to obtain a tissue block with the same diamet...

technical field

[0001] The invention relates to a method for making a tissue chip based on one-shot molding, which is used for preparing a tissue chip and belongs to the technical field of tissue chips. Background technique

[0002] Tissue chip (tissue chip) is an important branch of biochip technology, which can arrange dozens or even thousands of clinical tissue specimens of different individuals on a slide for analysis and research in a pre-designed order, and conduct the original analysis of the same index. Bit histological research is a high-throughput, multi-sample analytical tool. Tissue chip technology can analyze hundreds or even thousands of tissue samples at the same time, and conduct research on the expression of one or more specific proteins. Since the technology came out in 1998, it has been popularized and applied in a wide range due to its large-scale, high-throughput, and standardization advantages. Tissue chips together with gene chips and protein chips c...

Examples

Embodiment Construction

[0016] The present invention will be described in detail below in conjunction with the examples.

[0017] 1. Prepare the mold frame and embedding frame for making tissue chips:

[0018] Such as figure 1 Shown is a schematic diagram of the tissue chip casting mold frame, which is composed of a chip array orifice plate and an embedding tube. There are supports around the mold frame, and the height of the position is controlled by nuts. The embedding frame of the tissue chip is composed of two vertically bent copper strips, with a length of 70mm, a width of 40mm, and a height of 20mm, which can be freely separated and assembled into rectangles with different internal diameters.

[0019] 2. Tissue sampling:

[0020] According to the tissue to be prepared, find out and mark the target tissue area. According to the size of the target area, select a flat knife with an appropriate width, and cut vertically downward along the marked line to obtain a tissue block with the same diamet...

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