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Method for studying microstructure of clonorchis sinensis egg in gallstone

A technology of Clonorchis sinensis and gallbladder stones, which is applied in the field of research on the microstructure of Clonorchis sinensis eggs in gallbladder stones, can solve the problem of lack of microstructure research on the formation mechanism of Clonorchis sinensis eggs and gallbladder stones, and gallbladder stones are not very clear And other issues

Inactive Publication Date: 2013-03-27
GUANGZHOU BAODAN MEDICAL INSTR TECH
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Problems solved by technology

In recent years, the research on the causes of gallstones has made great progress. For example, Qiao Tie et al. reported that the eggs of Clonorchis sinensis are closely related to gallstones, but the formation mechanism of gallstones is still not very clear. Microstructural Study of Orchid Fluke Eggs and the Mechanism of Gallstone Formation

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  • Method for studying microstructure of clonorchis sinensis egg in gallstone
  • Method for studying microstructure of clonorchis sinensis egg in gallstone
  • Method for studying microstructure of clonorchis sinensis egg in gallstone

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Embodiment Construction

[0024] The method for researching the microstructure of C. sinensis ovum of gallbladder stone of the present invention, its concrete steps are:

[0025] (1) Prepare gallbladder stone specimens routinely and perform coating.

[0026] (2) Place the specimen table in the scanning electron microscope sample chamber, extract the negative pressure, turn on the filament, and after focusing, first locate the gallbladder stone specimen at 20-40 times.

[0027] (3) Select local gallbladder stone specimens and enlarge them to 400 times in order to find Clonorchis sinensis eggs and various crystals. Such as figure 1 , 2 The microscopic tissue pictures are shown, in which 1 is Clonorchis sinensis eggs, 2 is calcium carbonate crystals, and 3 is bilirubin and salt particles. Since the average size of Clonorchis sinensis eggs is 29 μm×17 μm, the diameter of calcium carbonate crystals in gallbladder stones is 5-15 μm, the thickness of cholesterol crystals is 0.2-2 μm, and the bilirubin and ...

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Abstract

The invention belongs to a medical research method, and specifically discloses a method for studying a microstructure of a clonorchis sinensis egg in gallstone. The method comprises the steps that: (1) a gallstone specimen is prepared with a conventional method, and is filmed; (2) the gallstone specimen is placed in a sample chamber of a scanning electron microscope, and is positioned under a magnification of 20-40 times; (3) a local gallstone specimen is respectively magnified to 400 times, 1000 times, 3000times, 6000 times, 10000 times and 20000 times, wherein scanning electron microscope observation and X-ray energy spectrometer analysis are carried out. According to the invention, with the scanning electron microscope and the X-ray energy spectrometer, the microstructure of the gallstone clonorchis sinensis egg is studied. According to the size and morphology of clonorchis sinensis egg and size and morphology of gallstone crystal, ordered and gradually magnified analysis is carried out, and a novel research method is provided for the discussion of clonorchis sinensis egg and gallstone formation mechanism.

Description

technical field [0001] The invention relates to a medical research method, in particular to a method for studying the microscopic structure of clonorchis sinensis eggs of gallbladder stones. Background technique [0002] Gallbladder stone disease is one of the most common digestive system diseases in the world. With the increasing incidence, it seriously affects people's health and quality of life. In recent years, the research on the causes of gallstones has made great progress. For example, Qiao Tie et al. reported that the eggs of Clonorchis sinensis are closely related to gallstones, but the formation mechanism of gallstones is still not very clear. Microstructural studies of Orchis sinensis eggs and the mechanism of gallstone formation. Contents of the invention [0003] The invention provides a method for studying the microstructure of Clonorchis sinensis eggs in gallbladder stones. The microstructure of Clonorchis sinensis eggs in gallbladder stones is analyzed by ...

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Application Information

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IPC IPC(8): G01N23/22G01Q30/02
Inventor 乔铁谢景夏罗小兵马瑞红
Owner GUANGZHOU BAODAN MEDICAL INSTR TECH
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