Method for artificially fermenting tobacco leaves
A technology of artificial alcohol and tobacco leaves, which is applied in the fields of tobacco, tobacco treatment, application, etc., can solve the problems of harsh reaction conditions, side effects, and limited application, and achieve the effects of reducing production costs, simple operation, and promoting alcoholization
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Embodiment 1
[0030] experiment material
[0031] Unfermented tobacco leaves, local tobacco leaves fermented for 1, 2, and 3 years (all produced in Yunnan), sterile water, nutrient broth medium, PDB medium.
[0032] method
[0033] 1. After surface disinfection of 1-year-old fermented tobacco leaves, mash them, dilute them with sterile water and pour them into the above nutrient broth medium, 37°C, 1d; PDB medium, 28°C, 3d shaker culture. The treatment of 2-year fermented tobacco leaves and 3-year fermented tobacco leaves is the same as above;
[0034] 2. Tobacco leaf thalli obtained from fermentation in nutrient broth culture medium for one year, two years, and three years were combined in a mass ratio of 1:1:1;
[0035] 3. Tobacco leaf thalli were fermented for one year, two years, and three years respectively by using PDB medium to culture the bacteria and merge the thalline according to the mass ratio of 1:1:1;
[0036] 4. The cells obtained in steps (3) and (4) are combined again a...
Embodiment 2
[0039] experiment material
[0040] Unfermented tobacco leaves, local tobacco leaves fermented for 1, 2, and 3 years (produced in Yunnan), sterile water, nutrient broth medium, PDB medium.
[0041] method
[0042] 1. After surface disinfection of 1-year-old fermented tobacco leaves, mash them, dilute them with sterile water and pour them into the above nutrient broth medium, 37°C, 1d; PDB medium, 28°C, 3d shaker culture. The treatment of 2-year fermented tobacco leaves and 3-year fermented tobacco leaves is the same as above;
[0043] 2. Tobacco leaf thalli obtained from fermentation with nutrient broth culture medium for one year, two years, and three years were combined in a mass ratio of 1:2:5;
[0044] 3. Tobacco leaf cells obtained from fermentation for one year, two years, and three years were respectively combined by mass ratio of 1:2:5 with PDB medium;
[0045] 4. The cells obtained in steps (3) and (4) are combined again and then added to unfermented tobacco leave...
Embodiment 3
[0048] experiment material
[0049] Unfermented tobacco leaves, local tobacco leaves fermented for 1, 2, and 3 years (produced in Yunnan), sterile water, nutrient broth medium, PDB medium.
[0050] method
[0051] 1. After surface disinfection of 1-year-old fermented tobacco leaves, mash them, dilute them with sterile water and pour them into the above nutrient broth medium, 37°C, 1d; PDB medium, 28°C, 3d shaker culture. The treatment of 2-year fermented tobacco leaves and 3-year fermented tobacco leaves is the same as above;
[0052] 2. Tobacco leaf thalli obtained from fermentation in nutrient broth culture medium for one year, two years, and three years were combined in a mass ratio of 1:3:6;
[0053] 3. Tobacco leaf cells obtained from fermentation for one year, two years, and three years were respectively combined by mass ratio of 1:3:6 with PDB medium;
[0054] 4. The cells obtained in steps (3) and (4) are combined again and then added to unfermented tobacco leaves ...
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