Method for synthesizing ester by using cutinase

A cutinase and reaction system technology, applied in the field of enzyme engineering, can solve the problems of high enzyme addition and low conversion rate of long-chain ester synthesis

Active Publication Date: 2013-04-03
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There have been reports on the application of cutinase derived from the fungus F.solani in the synthesis of short-chain esters. Among them, the conversion rate of ethyl hexanoate synthesis is the highest, which is 97%, but the amount of enzyme added is very high, which is 240U / mL, and The conversion rate of ester synthesis of medium and long chain is very low

Method used

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  • Method for synthesizing ester by using cutinase
  • Method for synthesizing ester by using cutinase
  • Method for synthesizing ester by using cutinase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] In this example, the cutinase Tfu_0883 derived from Thermobifida fusca was used to catalyze the reaction.

[0016] Organic reagents and substrate acids and alcohols are used for ester synthesis after removing water with 4A molecular sieve. Reaction conditions: 0.1M acid, 0.2M alcohol, 0.03% water added, 50°C, 150rpm, enzyme 30U / ml, reaction solvent isooctane, the specific results are shown in Table 1 and Table 2.

[0017] Table 1

[0018]

[0019]

[0020] Table 2

[0021]

Embodiment 2

[0023] In this example, the cutinase Tfu_0882 derived from Thermobifida fusca was used to catalyze the reaction.

[0024] Reaction conditions: 0.15M acid, 0.3M alcohol, 0.05% water addition, 50°C, 150rpm, enzyme 40U / ml, reaction solvent isooctane, the specific results are shown in Table 3 and Table 4.

[0025] table 3

[0026]

[0027] Table 4

[0028]

Embodiment 3

[0030] In this example, the cutinase Tfu_0883 derived from Thermobifda fusca was used to catalyze the reaction.

[0031] Reaction conditions: 0.5M acid, 0.5M alcohol, 0.08% water added, 50°C, 150rpm, enzyme 60U / ml, reaction solvent isooctane. After reacting for 5h and 15h, the conversion rates of propyl butyrate were 92.8% and 91.5%, respectively.

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Abstract

The invention discloses a method for synthesizing ester by using cutinase. The Thermobifida fusca cutinase is added in a reaction system of acid and alcohol, wherein the cutinase is from Thernobifida fusca and the NCBI (national center of biotechnology information) number is AAZ54920 and AAZ54921; and the reaction system is as follows: the reaction solvent is an organic solvent, the additive amount of water is 0.03-0.08%, and the addition amount of enzyme is 10-80U/mL. The method disclosed by the invention is simple in technology and has the advantages of simple process, high conversion rate, wide application scope, and the like.

Description

Technical field [0001] The invention relates to a method for synthesizing esters by using cutinase, and belongs to the technical field of enzyme engineering. Background technique [0002] Esters are a class of compounds produced by the reaction of acids and alcohols to lose water. They are widely used in food, wine, beverages, medicine, health care products, daily chemicals and other fields. At present, the production of ester substances basically adopts chemical methods, which are obtained by catalyzing the esterification reaction of acids and alcohols by inorganic catalysts (such as concentrated sulfuric acid) at high temperatures. Although the production cost is low, some uncontrollable side reactions in the process make the product quality low. The corrosion of the equipment by strong acid will also increase maintenance costs. More importantly, the environmental pollution caused by this method is not in line with today's sustainable development. Mainstream. The enzymatic sy...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/62
Inventor 吴敬宿玲恰陈坚
Owner JIANGNAN UNIV
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