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Staphylococcus saprophyticus selective culture medium and preparation method thereof

A selective technology for Staphylococcus saprophyticus, which is applied in the field of medical inspection, can solve the problems that Staphylococcus saprophyticus cannot be selectively cultivated, Staphylococcus saprophyticus cannot grow, etc., and achieve reliable detection and high separation rate

Inactive Publication Date: 2013-04-03
朱义江
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this medium cannot selectively cultivate Staphylococcus saprophyticus, and its reason is that Staphylococcus aureus grows faster than Staphylococcus saprophyticus in the current selective medium, covering the medium soon, and Staphylococcus saprophyticus cannot grow

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Example 1, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; Ketone 0.5 g; Potassium dihydrogen phosphate 0.2 g; Add distilled water to 1000 ml. Preparation method: take malt extract powder; egg white liquid; sodium chloride; magnesium citrate; agar; sterilized at low temperature for 2 times, each time for 45 minutes, and packed after sterilization.

Embodiment 2

[0027] Example 2, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; Ketone 0.5 g; Potassium dihydrogen phosphate 0.2 g; Copper sulfate 5 mg; Add distilled water to 1000 ml. Preparation method: take malt extract powder; egg white liquid; sodium chloride; magnesium citrate; , distilled water to volume, low-temperature sterilization twice, 45 min each time, and then packed after sterilization.

Embodiment 3

[0028] Example 3, the formula for preparing 1000ml of selective medium contains: 15g of malt extract powder; 150ml of egg white liquid; 5g of sodium chloride; 0.5g of magnesium citrate; 15g of agar; 0.5 g ketone; 0.2 g potassium dihydrogen phosphate; 0.1 g L-cysteine; add distilled water to 1000 ml. Preparation method: take malt extract powder; egg white liquid; sodium chloride; magnesium citrate; agar; After mixing and filtering, make up to volume with distilled water, sterilize twice at low temperature, 45 min each time, and pack after sterilization.

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PUM

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Abstract

The invention discloses a Staphylococcus saprophyticus selective culture medium and a preparation method thereof. The invention is characterized in that the formula of every 1000ml of selective culture medium comprises the following components: 15g of malt extract powder, 150ml of egg white liquid, 5g of sodium chloride, 0.5g of magnesium citrate; 15g of agar, 8-12mg of coetsoidin A, 0.5g of arborinone, 0.2g of potassium dihydrogen phosphate and the balance of distilled water. The optimal preparation method of the formula comprises the following steps: dissolving the malt extract powder, the egg white liquid, sodium chlorid, magnesium citrate, the agar, coetsoidin A, arborinone, potassium dihydrogen phosphate, L-cysteine and copper sulfate in a lily boiled liquid, evenly mixing, regulating to a constant volume, sterilizing, and then subpackaging. Compared with the prior art, the invention has the characteristics of high detection reliability and high Staphylococcus saprophyticus separation rate.

Description

technical field [0001] The invention relates to the field of medical examination, in particular to a selective culture medium for Staphylococcus saprophyticus and a preparation method thereof. Background technique [0002] Staph.saparophytics is a low-virulence opportunistic pathogen. Symptoms after infection are not typical, and multi-drug resistance is present, which brings certain difficulties to clinical diagnosis and treatment. Efficient and reliable detection methods are inevitable. [0003] The so-called microbial culture is a technology that uses artificial methods to grow and reproduce microorganisms, and selective selective medium is a selective medium designed to promote or inhibit certain types of organisms (such as cells or bacteria, etc.). This selective medium allows the separation of desired microorganisms from promiscuous microorganisms. [0004] Manitol Salt Agar is the commonly used selective culture medium for Staphylococcus aureus, which is used for the...

Claims

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Application Information

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IPC IPC(8): C12Q1/14C12R1/44
Inventor 候素君
Owner 朱义江