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Method for preparing avian infectious bronchitis positive serum from domestic rabbit

A technique for bronchitis, chicken contagiousness

Inactive Publication Date: 2013-04-03
山东滨州沃华生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation of specific serum requires high standards for SPF animals and is difficult to obtain
However, the specific serum prepared by using ordinary homologous animals as immune receptors is easily contaminated by other viruses, which will affect the specificity and reliability of the serum.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0015] In the examples of the present invention, unless otherwise specified, all parts and percentages are calculated by volume.

[0016] Materials: The infectious bronchitis virus strains used to manufacture this product are H120 strain and H52 strain, and the M41 antigen purchased in the Netherlands is used for detection, and a healthy rabbit with a body weight of 2.5kg.

[0017] Implementation steps:

[0018] (1) First mix the H120 strain and the H52 strain in equal amounts, make a 3-fold dilution, and then inject 0.5ml into the ear vein of the rabbit, collect 0.5ml of blood 7 days after the injection, and the detection antibody is 2 2 .

[0019] (2) The second immunization was carried out 7 days after the first immunization, the H120 strain and the H52 strain were mixed in equal amounts, diluted twice, and then 1ml was injected into the ear vein of the rabbit, and 0.5ml of blood was collected 7 days after the injection , the detection serum antibody is 2 7 .

[0020] (...

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PUM

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Abstract

The invention relates to a method for preparing avian infectious bronchitis positive serum from a domestic rabbit. According to the method, the domestic rabbit is creatively used as an immunoreceptor for preparing the avian infectious bronchitis positive serum, and the immunizing dose, the immunizing frequency, the immunizing interval, the immunizing way, the blood-taking time, the serum treatment, the detection and the like in the serum preparation process are systematically adjusted. The titer of an antibody obtained by the avian infectious bronchitis positive serum prepared from the domestic rabbit is high, the homologous pollution is avoided, and the quality is stable; and furthermore, the method has the advantages of simplified conditions, convenience in operation, capability of saving time and raw materials, easiness in implementation and good social benefits and social benefits.

Description

technical field [0001] The invention relates to the technical field of breeding biological products for animals, in particular to a method for preparing chicken infectious bronchitis positive serum with rabbits. Background technique [0002] Specific serum neutralization test is a commonly used method in the identification test of live vaccines and exogenous viruses in biological products. At present, when preparing specific serum at home and abroad, homologous SPF animals are usually used as immune receptors. The so-called SPF animals, that is, animals without specific pathogens (meaning that there are no specific microorganisms and parasites in animals) are animals that do not carry potential infection or conditional pathogenic bacteria, but carry non-specific microorganisms and parasites. The preparation of specific serum requires high standards for SPF animals and is difficult to obtain. However, the specific serum prepared by using common-grade homologous animals as i...

Claims

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Application Information

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IPC IPC(8): G01N33/531
Inventor 孟照洁马景霞贾红梅
Owner 山东滨州沃华生物工程有限公司
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