Jiang chuan mycin for preventing and controlling plant diseases
A technology of Jiangchuanmycin and plant diseases, applied in the field of pesticide technology and fermentation, can solve the problems of no one reporting the function of pesticides, unclear effects, loss of antibiotic activity, etc., to achieve convenient practical control operations, good adaptability of application, and tolerance Good rain wash effect
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Embodiment 1
[0048] Example 1 Fermentation preparation and identification of Jiangchuanmycin
[0049] Inoculate the Streptomyces griseochromogenes strain CGMCC No. 6829, which the applicant deposited on November 15, 2012, at the General Microorganism Center (CGMCC) of the Chinese The weight percentage formula is: glucose 2%, starch 5%, peanut cake powder 2%, soybean cake powder 1%, peptone 0.1%, yeast extract 0.4%, sodium chloride 0.2%, magnesium sulfate 0.5%, ammonium nitrate 0.2% , disodium hydrogen phosphate 0.05%, pH 7.2, the balance is water), and cultured on a shaking table at 28° C. at 300 rpm for 64 hours to obtain a cultured seed solution.
[0050] The seed solution was inoculated into the fermentation medium in the fermenter with 10% inoculation amount (V / V) (the formula by weight percentage is: glucose 0.4%, starch 7%, peanut cake powder 1.5%, soybean cake powder 1.5% , 0.1% peptone, 0.4% yeast extract, 0.2% sodium chloride, 0.5% magnesium sulfate, 0.2% ammonium...
Embodiment 2
[0056] The mensuration of the antibacterial spectrum of embodiment 2 Jiangchuanmycin
[0057] Cultivate pathogenic microorganisms on plants mixed with different concentrations of Jiangchuanmycin (100, 50, 25mg / L), measure the colony growth diameter of each pathogenic bacteria, and compare with each blank control (the medium does not contain Jiangchuanmycin) Comparing to calculate inhibition rate. The measured pathogens except cucumber powdery mildew ( Sphaerotheca fuliginea , which will be studied in detail below), also includes: Xanthomonas oryzae (Pseudomonas oryzae), Erwinia carotovora (Cabbage Soft Rot), Bacillus subtilis (Bacillus subtilis), Eshchcrichia coli (Escherichia coli), Helmintosporium maydis (Maize subspecies fungus), Pyricuraria oryzae CaV (Ryza sativa), Colletotrichum lagenarium (Anthracnose of melons), Phoma asparagi saccardo (Asparagus Stem Blight), Helminthosporium oryzae (Rice flax fungus), Alternaria solani (Tomato blight ...
Embodiment 3
[0061] Example 3 Control effect test of Jiangchuanmycin on melon anthracnose
[0062] Spray equal volumes of Jiangchuanmycin of different concentrations on a cucumber seedling at the true leaf stage in advance, and then artificially inoculate the anthracnose conidia suspension (spore concentration of 6.0×10 5 pcs / ml), and then put the cucumber seedlings in a constant temperature room (about 24°C) in a humidity chamber for shading and moisture induction. After 42 hours, take the cucumber seedlings out of the humidity chamber and place them under the light of a constant temperature room until the 7th day as a blank control ( (without pre-spraying Jiangchuanmycin) after the condition stabilized, count the number of anthracnose lesions on the leaves, compare with the blank control, calculate the control effect and further calculate the EC against melon anthracnose by linear regression method 50 value.
[0063] Table 2 Control effect of Jiangchuanmycin on melon anthracnose
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