Method for identifying cigarettes by using electrochemical fingerprints
A fingerprint and electrochemical technology, applied in the field of tobacco identification, can solve the problems of complex spectra, expensive instruments, difficult operation, etc., and achieve the effect of simple sample processing, accurate and reliable results, and simple and convenient operation
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Embodiment 1
[0027] The method for identifying cigarette varieties by using electrochemical fingerprints, comprising the steps of:
[0028] A. Preparation of Cigarette Samples
[0029] Take the shredded tobacco of Longfeng Chengxiang (soft Charm Chaotianmen), Jiaozi (soft Sunshine) and Zhonghua (hard) respectively, and dry it for 30 minutes at a temperature of 110°C. After cooling, add 50mL extract to 1g of shredded tobacco, and stir 15 minutes, then filter with gauze, collect the filtrate, get the standard sample, wherein the extract is the concentration of 0.5mol / L H 2 SO 4 ;
[0030] B. Obtaining Fingerprints
[0031] Take 8.00 mL of the standard sample obtained in step A and add it to the B-Z oscillating system, control the solution temperature at 37.00±0.05°C, use a platinum electrode as the indicator electrode, and a double-salt bridge saturated calomel electrode as the reference electrode, and obtain the potential change curve with time. That is, to obtain the electrochemical fi...
Embodiment 2
[0038] The method for identifying cigarette varieties by using electrochemical fingerprints, comprising the steps of:
[0039] A. Preparation of Cigarette Samples
[0040] Take the shredded tobacco of Zhenzhonghua (hard), Taijian Zhonghua (hard) Ⅰ and Taijian Zhonghua (hard) Ⅱ respectively, and dry it for 30 minutes at a temperature of 110°C. After cooling, add 50mL of extracting solution to 1g of shredded tobacco, and stir. After 15 minutes, filter with gauze and collect the filtrate to obtain the standard sample of Zhonghua (hard), the sample of Zhonghua (hard) I and the sample of Zhonghua (hard) II to be identified, wherein the extract is 0.5mol / L H 2 SO 4 ;
[0041] B. Acquisition of Fingerprints
[0042] Take 8.00 mL of the standard sample and the sample to be identified from step A respectively and add them to the B-Z oscillation system. The change curve of time, that is, to obtain the electrochemical fingerprints of the standard sample and the sample to be identifie...
Embodiment 3
[0049] The method for identifying cigarette varieties by using electrochemical fingerprints, comprising the steps of:
[0050] A. Preparation of Cigarette Samples
[0051] Cut tobacco from Longfeng Chengxiang (soft charm Chaotianmen), Jiaozi (soft sunshine), Zhonghua (hard), Taijian Zhonghua (hard) Ⅰ and Taijian Zhonghua (hard) Ⅱ cigarettes were respectively taken, and the temperature was 60°C , dried for 60 minutes, after cooling, add 10mL extract to 1g shredded tobacco, stir for 5 minutes, then filter with gauze, collect the filtrate, and obtain the standard sample of Longfeng Chengxiang (soft charm Chaotianmen), Jiaozi (soft sunshine), standard sample of Zhonghua (Hard) standard sample, Zhonghua (hard) I sample and Zhonghua (hard) II sample, in which the extract is H with a concentration of 0.2mol / L 2 SO 4 ;
[0052] B. Obtaining Fingerprints
[0053] Take 10mL of the standard sample obtained in step A and add it to the B-Z oscillation system. Under the condition of tem...
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