39 results about "Pulmonary tb" patented technology

The invention relates to a traditional Chinese medicine for treating pulmonary tuberculosis, which belongs to the field of medicines. The invention aims to provide the traditional Chinese medicine for treating pulmonary tuberculosis, which adopts the theory of traditional Chinese medicine and has the effects of nourishing Yin and moistening lung, regulating functions of an organism, relieving cough and reducing sputum and the like. In the invention, the prepared traditional Chinese medicine comprises the following active ingredients: dwarf lilyturf tuber, root of straight ladybell, lily, bulbus fritilariae, manyflower solomonseal rhizome, yam, sessile stemona root, bletilla and platycodon root. The traditional Chinese medicine has unique compatibility, can regulate the overall functions of the organism, has the effects of nourishing Yin and moistening lung and relieving cough and reducing sputum, has the effective rate of 83.33 percent and is used for treating pulmonary tuberculosis and yin deficiency of lungs. The traditional chinese medicine provided by the invention is used for treating symptoms containing tussiculation, night sweat, feverish palms and soles, sputum with blood streaks or blood sports, chest ache, dry mouth and lips, shortness of cough, coughing with little viscous sputum, red cheek, dry skin, red tongue and shortage of the production of body fluid, thready pulse or weak or intermittent pulse and the like.

The invention provides a diagnostic kit for detecting active pulmonary tuberculosis. The kit consists of an RNA extract buffer solution, a specific serum miRNA composition for the active pulmonary tuberculosis, internal reference reverse transcription primers, polymerase chain reaction (PCR) primers and a fluorescent quantitative reverse transcription-polymerase chain reaction (RT-PCR) reaction solution, wherein the specific serum miRNA composition for the active pulmonary tuberculosis consists of four differential expression serum miRNAs, namely hsa-miR-29c, hsa-miR-22, hsa-miR-320b and hsa-miR-101. By using the specific serum miRNAs composition for the active pulmonary tuberculosis to detect the active pulmonary tuberculosis on the serum miRNAs level, the sensitivity is 90.2 percent, and the specificity is 75.0 percent; and the kit has early-stage diagnostic value for the active pulmonary tuberculosis, and can realize early warning and early diagnosis of the active pulmonary tuberculosis.

The invention discloses a serum miRNA combination-based pulmonary tuberculosis therapeutic effect evaluation kit and applications thereof. The kit comprises an RNA extraction buffering solution, a Poly (A)-containing reaction solution, a reverse transcription reaction solution, a serum miRNA upstream primer combination, an internal reference upstream primer, a universal downstream primer and a fluorescent quantitation RT-PCR reaction solution, wherein the serum miRNA upstream primer combination comprises four differentially expressed serum miRNA: hsa-miR-21-5p, hsa-miR-92a-3p, hsa-miR-125a-5p and hsa-miR-148b-3p, the sequences of which are respectively SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3 and SEQ ID NO:4; the internal reference is hsa-miR-16, the sequence of which is SEQ ID NO:5. The detection sensitivity of the kit on treatment of pulmonary tuberculosis is 65.38%, and the specificity is 80.77%, so that the specificity is strong and the sensitivity is high, and the kit has higher accuracy, and is simple and convenient to operate, high in efficiency, small in specimen state limit, and superior to the sputum culture currently clinically adopted, thus providing a new evaluation method for the evaluation on the therapeutic efficacy of pulmonary tuberculosis.

The invention relates to a medicament for treating pulmonary tuberculosis by combined chemotherapy with traditional Chinese medicine Fuying and a preparation method; in the medicament, a traditional Chinese medicine of Fuying No. I comprises gecko, donkey-hide gelatin, stemona, adenophora stricta, radix ophiopogonis, prunella vulgaris, radix scrophulariae, fritillaria thun-bergli, cortex lycii radicis, bletilla striata, picrorhiza scrophulariiflora pennell, rehmanniae radix, and anemarrhena, and is mainly used for expelling evil and nourishing yang; a traditional Chinese medicine of Fuying No. II comprises codonopsis pilosula, Chinese yam, poria cocos, schisandra chinensis, radix rehmanniae preparata, angelica, caulis spatholobi, gentrin knotweed, bighead atractylodes rhizome, and radix astragali, and is mainly used for strengthening the body resistance and nourishing body; western medicines participating the combined chemotherapy comprise: streptomycin injections, isoniazid tablets, rifampin, and pyrazinamide capsules; during the combined chemotherapy with the medicines, not only stable therapeutic effect on pulmonary tuberculosis is realized, but also nourishing treatment effect on patients with hepatopathy is realized; during preparation, geckoes are grinded into fine powder, and fried with donkey-hide gelatin; then the other medicines are cleaned, dried, pulverized, and grinded into fine powder; the fine powder is sieved by a No. 6 sieve to obtain a pulvis; the pulvis is packaged into bags to obtain the Fuying No. I. During the preparation of the Fuying No. II, the medicines are also selected, cleaned, pulverized, and grinded into fine powder; the fine powder is sieved by a No. 6 sieve to obtain a pulvis; the pulvis is packaged into bags to obtain the Fuying No. II. During treatment, the traditional Chinese medicine Fuying is combined with western medicines for administration.

A sputum treating liquid for inspecting standard films of Mycobacterium tuberculosis in sputum comprises ethyl alcohol, isopropyl alcohol, boric acid, disodium EDTA, SDS, a mucus dissolving agent and distilled water and is capable of dissolving phlegm, deactivating pathogenic bacteria such as Mycobacterium tuberculosis as well as viruses in sputum, preventing corrosion and splitting exfoliative cells in sputum. Applied with simple centrifugal operation, the sputum treatment with the sputum treating liquid enables 8 to 10 spits of sputum to be treated at a time, and even sputum left for 24 hours, the treatment quantity of sputum is increased, standard operation is achieved through a standard automatic film-making technique, the operation is simple and easy, detection sensitivity is improved, detection is accurate and reliable, and the sputum treating liquid is suitable for the general investigation of tuberculosis high-risk groups, screening of rural tuberculosis and clinical diagnosis of tuberculosis.

A composite Chinese medicine in the form of dripping pill and soft capsule for treating infiltrative pulmonary tuberculosis and blood-stained sputum, and its preparing process are disclosed.

The invention discloses one group of nucleic acid aptamers capable of specifically bonding with tuberculosis mycobacterium Beijing genotype strain surface lipolysaccharide namely mannose modified mannosylated lipoarabinomannan (ManLAM) and application of the nucleic acid aptamers. The aptamers are specific to small-molecular single-chain DNA (ssDNA) of the ManLAM, and have a nucleotide sequence as shown in the SEQ ID No.1-5. The ssDNA has functions similar to those of a monoclonal antibody, and can be directly and specifically bonded with ManLAM. By adopting an enzyme-linked oligonucleotide assay (ELONA) method, the ManLAM antigen in serum of patients with pulmonary tuberculosis, extrapulmanory tuberculosis and the like can be quickly effectively detected in 2 hours, the ManLAM antigen in sputum of an active pulmonary tuberculosis patient can be directly identified, and the detection sensitivity on the Man LAM antigen can be 0.035mu g/mL. The aptamers are simple to prepare and low in cost, and can be used as an effective novel reagent for diagnosing the mycobacterium tuberculosis antigen of early and latent tuberculosis.

The invention discloses a pulmonary tuberculosis sampler, which comprises a supporting column, an electric telescopic rod, a cleaning box and a positive and negative motor, wherein a top cover is fixedly mounted above the supporting column, a sampling needle tube is connected to the middle of the upper part of the top cover, a piston rod is connected to the inner side of the sampling needle tube,a groove is formed in the lower portion of the left side of the piston rod, an electric telescopic rod is fixedly installed on the left side of the top cover, a sliding groove is formed in the inner surface of the supporting column, a rotating rod is rotatably connected to the bottom of the supporting column, and a connecting block is welded to the inner side of the cleaning box. According to thepulmonary tuberculosis sampler, the positive and negative motor can drive the rotating gear to be meshed with tooth blocks on the surface of the sampling needle tube, so that the sampling needle tubecan conveniently move up and down on the surface of the top cover, the sampler can be conveniently and automatically inserted into a cup for containing pathogen sputum of pulmonary tuberculosis, and the rod body of the sampler can conveniently extend upwards and conveniently drive the piston rod to move upwards for sampling operation by matching with the electric telescopic rod.

The invention discloses a breathing machine for internal medicine-tuberculosis. The breathing machine includes a disinfecting box; the top end of the disinfecting box is provided with an installationmouth; a filtering plate is slidingly installed in the installation mouth; the top end of the filtering plate extends to the external of the disinfecting box and is fixedly connected to a baffle plate; the top end of the baffle plate is fixedly connected to a pulling handle; the side, away from the installation mouth, on the top end of the disinfecting box is fixedly connected to a water adding port; a sealing cover is installed on the water adding port in a threaded manner; one side of the disinfecting box is respectively and fixedly connected to an air outlet pipe and a water outlet pipe; and the water outlet pipe is located under the air outlet pipe. The breathing machine is reasonable in structure and significant in beneficial effect, and has double disinfection effects; the tuberculosis viruses left on the disinfecting box and the filtering plate can be completely killed, so that the spreading of the viruses can be effectively isolated; the filtering plate can be conveniently taken out, so that the convenient replacing of the filtering plate can be realized; and a catheter can be conveniently stored in a catheter storage box, so that the dragging of the catheter due to the excessive long catheter can be effectively prevented from causing the twining and damaging of the catheter.

The invention discloses a classification device based on lipid biomarkers and application. Specifically, the classification device based on lipid biomarkers comprises a data acquisition module configured to acquire a feature data set of a patient; a model matching module configured to inquire a classification model library and determine a matched classification model according to the medication characteristics, wherein the classification model is obtained by training a preset classification algorithm according to expression quantities of lipid biomarkers in blood of different types of patients; and a patient classification module configured to obtain a classification result of the patient for pulmonary tuberculosis according to the classification model and the expression quantities of thelipid biomarkers in the blood. According to the technical scheme, harm to the body of a patient is low, the device can be suitable for early screening of pulmonary tuberculosis and evaluation of whether pulmonary tuberculosis is cured or not, and a new and objective classification scheme is provided for early screening diagnosis and curative effect evaluation of pulmonary tuberculosis.

The invention relates to a traditional Chinese medicine composition for treating dog tuberculosis. The traditional Chinese medicine composition is prepared from the following raw materials in parts by weight: 10-20 parts of platycodon grandiflorum, 11-20 parts of semen lepidii, 10-20 parts of Chinese-date, 12-20 parts of ephedra, 10-13 parts of gypsum, 10-20 parts of pinellia ternate and 10-14 parts of fresh ginger. The traditional Chinese medicine composition can be water aqua. The preparation method comprises the following preparation steps: (1) weighing various traditional Chinese medicines according to the formula ratio, and feeding to an extraction tank; (2) adding 5-15fold water to the extraction tank, and boiling twice and 2-12 hours each time; (3) filtering and merging filtrate of twice; (4) carrying out vacuum concentration at 30-80 DEG C until the volume is 10%-50% of original volume; and (5) filtering, deslagging and filling each bottle with100-200ml of liquid to obtain a finished product. The composition has the efficacies of relieving cough and asthma, eliminating phlegm, promoting circulation of qi, invigorating the spleen and replenishing qi; the traditional Chinese medicine composition provided by the invention is capable of treating exterior and interior of the dog tuberculosis, and is significant in curative effect; and the recovery rate can reach 90%.

The invention discloses a respirator for pulmonary tuberculosis patients. The respirator comprises a breathing mask, a processor and a corrugated pipe, wherein the breathing mask is provided with an air inlet and an air outlet, and a first activated carbon filter layer is arranged in the air inlet and used for filtering inhaled air; the processor comprises a shell and an ultrasonic nebulizer, the shell is provided with an atomization cavity and an exhaust cavity; the ultrasonic nebulizer is arranged in the atomization cavity and used for atomizing disinfectant in the atomization cavity to form atomized liquid medicine; atomization spray holes are formed between the exhaust cavity and the atomization cavity, and the atomized liquid medicine is sprayed into the exhaust cavity through the atomization spray holes; and one end of the corrugated pipe is connected to the air outlet, and the other end of the corrugated pipe is connected to the exhaust cavity, so that air exhausted from the air outlet is exhausted after being disinfected by the atomized liquid medicine in the exhaust cavity. The respirator for pulmonary tuberculosis patients provided by the embodiment of the invention can be used for performing disinfection and sterilization on exhaled air, and the infection risk can be greatly reduced.

The invention relates to the field of traditional Chinese medicine, in particular to traditional Chinese medicine for treating tuberculosis. Each dose of the traditional Chinese medicine for treating tuberculosis comprises the following medicinal herbs: 25 g of lily, 25 g of ainsliaea fragrans Champ., 25 g of radix stemonae, 25 g of bletilla, 25 g of humulus japonicus, 25 g of lucid asparagus, 15 g of cortex lycii radicis, 15 g of dwarf lilyturf turber, 30 g of heartleaf houttuynia herb, 30 g of siegesbeckia glabrescens makino, 25 g of petelot habenaria, 25 g of walnut kernel, 10 g of ginkgo, 15 g of osbeckia opipara, 10 g of longspur epimedium, 30 g of semen coicis, 25 g of alpinia japonica, 25 g of ceylon cynoglossum, 30 g of cirsium japonicum, 20 g of bistort, 50 g of cynoglossumfurcatum Wall., 15 g of Chinese arbor-vitae branches, 30 g of pilea notata and 30 g of liquorice. The traditional Chinese medicine for treating tuberculosis adopts the various traditional Chinese medicines to condition lungs, has the efficacies of nourishing yin, moistening lung, suppressing cough, reducing phlegm, cooling blood, stopping bleeding, removing blood stasis to regenerate tissues, regulating the flow of qi, relieving pain, benefiting a liver and kidneys, resisting bacterium, tonifying deficiency, and enhancing immunity, not only can resist tuberculosis from the source, but also can protect the lever, has obvious effects in treating symptoms such as cough, expectoration, hemoptysis and chest pain of tuberculosis, is free of toxic and side effects, and high in safety, and can treat both symptoms and root causes.

The invention discloses application of CD4+T lymphocyte characteristic protein to identification of active tuberculosis and latent tuberculosis infection (LTBI). The invention provides application ofa substance for detecting an expression quantity of integrin beta-3 and/or integrin-linked kinase to preparation of a product for distinguishing or identifying or assisting to distinguish or identifya patient with the active tuberculosis and a latent tuberculosis infection infected person. The invention finds out that integrin beta-3 and integrin-linked kinase protein can distinguish an LTBI patient and an initially-treated active tuberculosis patient, and novel diagnostic standards are provided for diagnosing the LTBI patient and the initially-treated active tuberculosis patient.

The invention discloses a pharmaceutical composition suitable for primary treatment of extra-short-range treatment of smear-positive pulmonary tuberculosis. The invention provides the pharmaceutical composition for treating primary treatment of smeared-positive pulmonary tuberculosis. The pharmaceutical composition is composed of levofloxacin, isoniazid, rifampicin, pyrazinamide and ethambutol. The pharmaceutical composition provided by the invention can be used for completing the primary treatment of the smeared-positive pulmonary tuberculosis through chemotherapy for four and a half months with a shorter range and higher efficiency, and compared with a standard six-month chemotherapy regimen, the pharmaceutical composition has the characteristic of high focus absorptivity, under the condition of ensuring a higher sputum culture negative conversion rate, a lower adverse reaction rate, a higher cure rate and a lower recurrence rate. According to the present invention, the treatment time of the primary treatment of the smeared-positive pulmonary tuberculosis can be shortened, and the pharmaceutical composition has important significance for reducing the prevalence and propagation oftuberculosis.

The invention discloses a medicinal composition for treating pulmonary tuberculosis, and a preparation method and a use thereof. The medicinal composition is prepared from Radix Glehniae, Radix Stemonae, Anemone chinensis, Radix Ranunculi Ternati, Common Boxwood, Cortex Lycii, centipedes, Fagopyrum cymosum, Orange Sugarmustard, Metaplexis japonica (Thunb.) Makino, Human Placenta, Veronica peregrina L., Fritillaria cirrhosa, deerhorn glue, turtle shell, Hibiscus trionum, Poria cocos, Meconopsis punicea, oyster, tingli seed and pork lung extract; the pork lung extract is one or more of phosphatidylcholine, dipalmitoylphosphatidylcholine, phosphatidylethanolamine, phosphatidylserine, phosphatidylinositol and sphingomyelin; and the effective component of Common Boxwood is cycloprotobuxinamin. The medicinal composition adopting traditional Chinese medicine and western medicine combination has the efficacies of moistening lung and relieving coughs, clearing heat and eliminating inflammation, cooling blood and stopping bleeding, and eliminating swelling and relieving pains, and has the advantages of substantial pulmonary tuberculosis treatment effect, safety, reliability, no toxic or side effects, short treatment course, fast effectiveness, low cost, and reaching of the purpose of simultaneous treatment of principal and subordinate symptoms.

The invention discloses a pulmonary tuberculosis mitochondria-derived small RNA (Ribonucleic Acid) detection kit. The pulmonary tuberculosis mitochondria-derived small RNA detection kit is characterized by comprising a pair of reverse transcription primers and detection primers corresponding to up-regulation of the expression ratio of mitochondria-derived small RNA and 6 pairs of down-regulation of the expression ratio of mitochondria-derived small RNA. The invention provides a specific expression profile of a mitochondrial-derived small RNA (mdsRNA) ratio in peripheral blood of a pulmonary tuberculosis patient, and a reverse transcription primer, a detection primer, a detection kit and a diagnosis model of the specific expression profile, and provides support for early discovery and early treatment of clinical pulmonary tuberculosis.

The invention discloses a serum metabolism marker related to pulmonary tuberculosis and application of the serum metabolism marker related to the pulmonary tuberculosis, and the serum metabolism marker related to the pulmonary tuberculosis comprises at least one of 3-oxopalmitic acid, akebia stem saponin, thiolithocholic acid, 2-decyl furan, (decahydro-4, 8, 8-trimethyl-1, 4-methylene azulene-9-subunit) methanol, D-(+)-camphor and 2-methylamino adenosine, and the serum metabolism marker related to the pulmonary tuberculosis comprises at least one of 3-oxopalmitic acid, akebia stem saponin, thiolithocholic acid, 2-decyl furan, (decahydro-4, 8, 8-trimethyl-1, 4-methylene azulene-9-subunit) methanol, D-(+)-camphor and 2-methylamino adenosine. According to the application, the serum metabolism marker is used for preparing a detection reagent or applied to detection related to pulmonary tuberculosis. The serum metabolism marker is suitable for detection related to pulmonary tuberculosis, the accuracy of the detection result is high, and the detection efficiency can be improved.

The invention discloses an excrement metabolite and detection system for detecting active pulmonary tuberculosis. The excrement metabolite is selected from 13 kinds of differential metabolites. The detection system is based on an excrement LC-MS metabonomics technology, LC-MS detection is carried out on excrement of a to-be-detected patient, the relative expression quantities of 13 different metabolites are obtained respectively, and then whether the to-be-detected patient is an active pulmonary tuberculosis patient or not is judged according to the relative expression quantities of the metabolites. Compared with the traditional method, the method provided by the invention has the advantages of non-invasion, simple sample treatment, no damage to the sample, small sample injection amount, good repeatability, low cost and the like.