Application of Phyllanthus urinaria polysaccharide in preparation of anti-hepatitis B virus medicines

A bead polysaccharide and anti-hepatitis B technology, which is applied in the field of medicine, can solve the problems of less adverse reactions of Chinese herbal medicine, low price, and needs further research, and achieve the effect of improving the body's immunity, less dosage, and high development value

Inactive Publication Date: 2014-05-07
FUJIAN AGRI & FORESTRY UNIV
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  • Abstract
  • Description
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Problems solved by technology

There are many varieties of traditional Chinese herbal medicines, which have been widely used in the clinical treatment of chronic hepatitis B and have achieved certain curative effects, and have accumulated rich clinical experience. However, the mechanism of Chinese herbal medicines against HBV needs to be further studied, so the screening of Chinese herbal medicines against HBV remains to be done. It has great potential; and the traditional Chinese herbal medicine has few adverse reactions and low price, and has been paid more and more attention by researchers at home and abroad. Seeking natural medicines with antiviral effects is a hot spot in current research

Method used

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  • Application of Phyllanthus urinaria polysaccharide in preparation of anti-hepatitis B virus medicines
  • Application of Phyllanthus urinaria polysaccharide in preparation of anti-hepatitis B virus medicines
  • Application of Phyllanthus urinaria polysaccharide in preparation of anti-hepatitis B virus medicines

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Embodiment Construction

[0015] Below in conjunction with embodiment the present invention is described in detail:

[0016] Extraction of Phyllostachys polysaccharide

[0017] The whole plant of Phyllostachys phylloxera was washed with distilled water to remove the soil, dried at 50°C, crushed, and sealed for later use. Its powder is yellow-green.

[0018] Petroleum ether reflux at 80 °C to degrease → 95% ethanol at 80 °C to remove small molecule sugars → 90 °C water to reflux for three times, combine the filtrate for three times and centrifuge at 4000r / min, concentrate to 1 / 4 volume → add 4 times the volume of 95% ethanol , stand still for 24 hours, centrifuge, redissolve in distilled water → remove protein by sevag method (chloroform: n-butanol = 4:1, polysaccharide solution: mixed solution = 3:1), magnetically stir for 30 minutes, fully stand for stratification, repeat 7~ Free protein can be removed after 8 operations → add 4 times the volume of 95% ethanol to the polysaccharide solution after ...

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Abstract

The invention discloses an application of a Phyllanthus urinaria polysaccharide PUIP III in the preparation of anti-hepatitis B virus medicines. The Phyllanthus urinaria polysaccharide PUIP III is composed of rhamnose, arabinose, mannose and glucose, the molar ratio of rhamnose to arabinose to mannose to glucose is 0.27:0.28:0.1:0.35, and the molecular weight of the Phyllanthus urinaria polysaccharide is 418793.5. The Phyllanthus urinaria polysaccharide is obtained through artificially extracting the medicinal plant Phyllanthus urinaria, separating and purifying. In-vitro biological activity experiments show that the PUIP III can inhibit the HBsAg and HBeAg secretion and the HBV-DNA duplication in the HepG2.2.2.15 cell culture, and has a certain in-vitro anti-HBV activity; and in-vivo activity experiments show that the PUIP III has an obvious inhibition effect on DHBVDNA in ducks, has small rebound, and can be used for preparing the anti-hepatitis B virus medicines and liver protection healthcare products.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the application of phyllophyllum polysaccharide PUIPⅢ in the preparation of anti-hepatitis B virus medicine. Background technique [0002] Hepatitis B Virus (HBV) has been discovered for more than 40 years. In 1963, Blumberg and others found that the serum of Australian hemophiliac patients contained an antigen that could react with the serum of American hemophiliac patients, calling it the Australian antigen (ie, Hepatitis B Virus Surface Antigen, Hepatitis B Virus Surface Antigen, HBsAg ). In 1970, Dane et al. found HBV particles with a size of 42nm in the serum of hepatitis B patients, and the outer membrane component contained HBsAg. In 1986, the International Committee on Virus Nomenclature officially classified HBV as a hepadnaviridae. [0003] Hepatitis B virus (HBV) infection is a worldwide health problem with worldwide distribution. It can not only cause acute and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61P31/20A23L1/29A23L33/00
Inventor 李清禄李凌峰李宇翔张丽丽谢勇平王玉林黄志坚
Owner FUJIAN AGRI & FORESTRY UNIV
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