Fermenting method for increasing dextranase enzyme activity unit

Abstract

The invention discloses a fermenting method for increasing dextranase enzyme activity units. In the fermenting process, the method comprises the following steps: continuously feeding a dextran solution to a fermentation medium when the total sugar content of the fermentation medium is reduced to 0.26%, wherein the content of the dextran is 0.5-1.5% of the total weight of the fermentation medium; continuing fermenting; continuously feeding the dextran solution to the fermentation medium when the total sugar content of the fermentation medium is reduced to 0.20%, wherein the content of the dextran is 0.5-1.5% of the total weight of the fermentation medium; and finishing the fermentation after continuing fermenting for 160-170 hours; and according to the invention, through supplementing a proper amount of dextrans in the later stage of the dextranase fermenting process as a persistent inducer for producing the dextranase, the enzyme production time is prolonged, the amplification of the enzyme activity unit is obvious and the maximum amplification reaches 18%; and the fermenting method for increasing the dextranase enzyme activity units, disclosed by the invention, has the advantages of strong operability, low input and high output.

Description

technical field [0001] The invention relates to the technical field of bioengineering, in particular to a fermentation method for increasing the enzyme activity unit of dextranase. Background technique [0002] Dextranase is a new enzyme that can catalyze the decomposition of dextran into glucose or oligosaccharides. It is a protein. Dextranase has important uses in the pharmaceutical industry and food industry. The study of dextranase is of great significance for the prevention and treatment of dental caries and the development of dental caries vaccine; The filterability of sugar products can increase the recovery rate of sugar and reduce the viscosity, so the market prospect of this product is broad. The existing dextranase fermentation method is to ferment continuously in a fixed fermentation medium for a certain period of time until the end of the fermentation. The medium is constant during the fermentation process. The enzyme activity unit of the dextranase obtained...

AI Technical Summary

Problems solved by technology

The existing dextranase fermentation method is to ferment continuously in a fixed fermentation medium for a certain period of time until the end of the fermentation. The medium is constant during the fermentation process. The enzyme activity unit of the dextranase obtained by this fermentation method is relatively low, and the enzyme activity The unit is between 450u / ml~460 u / ml