Application of sclerotium rolfsii scleroglucan fermentation broth as oil field drilling fluid treatment agent
A scleroglucan, oil field drilling technology, applied in the directions of fermentation, drilling composition, microorganism-based method, etc., can solve the problem of not finding Sclerotinia scleroglucan fermentation liquid and the like, achieve good compatibility, The effect of high purity and ability to withstand harsh formation conditions
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preparation example Construction
[0032] The preparation method of the Sclerotin scleroglucan fermentation liquid is an in-situ separation and fermentation method, and the steps are:
[0033] ⑴. Bacteria culture:
[0034] A 1000ml shake flask was used to cultivate the Sclerotinia seed solution in a constant temperature shaker. The components and mass percentages of the culture medium are: 4% sucrose, 0.3% sodium nitrate, 0.075% corn steep liquor, 0.075% yeast powder, 0.05% potassium chloride, 0.05% potassium dihydrogen phosphate, 0.05% magnesium sulfate heptahydrate %, the remainder is water, pH: 5.0. The temperature of the shaker was 30° C., the rotation speed of the shaker was 150 rpm, and the culture period was 48 hours.
[0035] ⑵. Using 30L fermenter to ferment Sclerotinia:
[0036] After the prepared fermentation medium is sterilized by a continuous sterilization device, it enters the fermenter after emptying and sterilizing. Sterilization conditions: 121°C, tank pressure: 1kg / cm 2 , the time is 20mi...
Embodiment 1
[0043] Mix the Sclerotin scleroglucan fermentation liquid with water according to the ratio of 10Kg and 90Kg of water to make an oilfield drilling fluid treatment agent. After testing, the Sclerotinia scleroglucan oilfield drilling fluid treatment agent prepared in the above ratio has a pH of 1- The viscosity remains unchanged under the condition of 13, and the pH range of xanthan gum, which is commonly used as oilfield products, is 3-11. Beyond this range, the viscosity drops by more than 30%, and the viscosity cannot be recovered. Therefore, as an oilfield drilling fluid treatment agent, Sclerotin scleroglucan is more suitable for oil well drilling with complex geological conditions.
Embodiment 2
[0045] Mix the Sclerotin scleroglucan fermentation broth with water at a ratio of 30Kg and 70Kg of water to make an oilfield drilling fluid treatment agent. After testing, the viscosity of the Sclerotinia scleroglucan oilfield drilling fluid treatment agent prepared in this ratio reaches 500cp , the viscosity recovery rate reaches 100% after high-shear stirring, and has good pseudoplasticity. However, polyacrylamide, which is also an oilfield product, will have a sharp drop in viscosity when stirred under high-shear force, and the viscosity cannot be recovered. , thus proving that Sclerotin scleroglucan has better pseudoplasticity than polyacrylamide.
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