Method for rapidly determining starch content of sorghum grains
A technology for rapid determination of starch content, applied in the measurement of color/spectral characteristics, etc., can solve the problems of error, deviation, and difference in absorbance, etc., and achieve the effect of high accuracy, high safety and low cost
Inactive Publication Date: 2015-05-13
GUANGXI DONGXING DINGKANG PLASTIC IND
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Problems solved by technology
Fan Mingshun et al. used dual-wavelength spectrophotometry to discuss the determination method of sorghum amylose and amylopectin content, but the absorption curves of the two starches in the visible light range vary with the concentration gradient, which makes the reference wavelength The absorbance at the measurement wavelength is not equal, resulting in a large deviation, and the mapping method to find the reference wavelength and the measurement wavelength will also produce a large error
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Embodiment 1
[0023] Embodiment 1 of the present invention: the method for quickly measuring sorghum grain starch content, amylose and amylopectin standard solution are carried out wavelength scanning, find out the absorption peak wavelength of amylose and amylopectin standard solution respectively; According to Lambert-Beer law, by drawing a standard curve, the molar absorptivity of the two starch components at the absorption peak position is obtained, and the simultaneous equations are used to solve the calculation formula of the two starch concentrations for rapid batch determination; the specific steps are as follows:
[0024] 1. Determination of absorption peak wavelength of amylose and amylopectin samples
[0025] (1) Weigh 100 mg of standard amylose and amylopectin respectively, add them into a 50 mL small beaker, moisten with 1 mL of absolute ethanol, add 9 mL of 1 mol / L NaOH solution, and disperse in a boiling water bath for 10 min , transferred to a 100 mL volumetric flask, clea...
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Abstract
The invention discloses a method for rapidly determining starch content of sorghum grains. The method comprises the following steps of: performing wavelength scanning on an amylose and amylopectin standard solution, respectively finding out the absorption peak wavelength value of the amylose and amylopectin standard solution; according to the Lambert-Beer law, drawing a standard curve to obtain the molar absorption coefficients of the two types of starch constituents at the absorption peak, and building simultaneous equations to solve the computational formula for two types of starch concentrations, wherein the computational formula is used for rapid batched determination. In the method, the colorimetric method is used, the content of amylose, the content of amylopectin and the content of total starch can be determined simultaneously, and the method is high in accuracy, convenient and efficient. The method provided by the invention solves the problem that the conventional starch determination method can only determine the content of the amylose or the content of the total starch each time, and can not simultaneously determine the content of the amylose and the content of the amylopectin; and moreover, the method established by the invention is free from application of concentrated sulfuric acid, concentrated hydrochloric acid and toxic reagents, thereby being more environment-friendly, high in safety, low in cost and ideal in using effect.
Description
technical field [0001] The invention relates to the technical field of crop starch analysis, in particular to a method for quickly measuring the starch content of sorghum grains. Background technique [0002] sorghum [ Sorghum bicolor (L.) Moench] is one of the oldest cereal crops in the world, belonging to the Gramineae (Gramineae) sorghum family ( Andropogoneae ) Sorghum ( sorghum ). In addition to being used as food, feed and energy crops, sorghum is also one of the main raw materials for the brewing industry. Sorghum is divided into japonica and waxy. The amylose, amylopectin and total starch content of different sorghum varieties vary greatly. important relationship. At the same time, the content and ratio of amylose and amylopectin also affect the cooking characteristics and taste, and the breeding of excellent wine-making types and edible varieties is of great significance in grain production and wine-making industries. Therefore, it is necessary to choose a f...
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IPC IPC(8): G01N21/33
Inventor 张明生李祥栋王洋
Owner GUANGXI DONGXING DINGKANG PLASTIC IND