An electrochemical sensor for mercury ion detection and its manufacturing method and detection method
A detection method and sensor technology, applied in the field of biological analysis, to achieve the effect of simple steps, high specificity, and simple operation
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Embodiment 1
[0037] Example 1: Construction of Hg when the whole thymine (T) oligonucleotide chain assembled on the surface of the gold electrode is low density 2+ Detection of electrochemical sensors.
[0038] The unmodified clean gold electrode was soaked in 10 mM Tris containing 1 μM probe 1 that had been reduced with tris[2-carboxyethyl]phosphine (TCEP) for 1 h, 0.2 M NaCl, pH=8.0, and left at room temperature for 1 h. The assembled electrode was rinsed twice with 10mM Tris, 0.5M NaCl, pH=8.0, then placed in an aqueous solution containing 1mM mercaptohexanol (MCH) to block for 30 minutes, and then washed three times with 10mM Tris, 0.5M NaCl, pH=8.0 , and then reacted with 500nM probe 2 in 1 / 15MPB, 0.3M NaCl, pH=7.4 solution for 2h at 25°C. Then wash the electrode three times with 1 / 15MPB, 0.1M NaCl, pH = 7.4 solution and store it at 4°C for future use.
Embodiment 2
[0039] Example 2: Construction of Hg when the whole thymine (T) oligonucleotide chain assembled on the surface of the gold electrode is medium density 2+ Detection of electrochemical sensors.
[0040] Soak an unmodified clean gold electrode in 10 mM Tris containing 1 μM probe 1 that has been reduced with tris[2-carboxyethyl]phosphine (TCEP) for 1 h, 1.0 M NaCl, pH=8.0, and place it at room temperature overnight. Assemble. The assembled electrode was rinsed twice with 10mM Tris, 0.5M NaCl, pH=8.0, then placed in an aqueous solution containing 1mM mercaptohexanol (MCH) to block for 30 minutes, and then washed three times with 10mM Tris, 0.5M NaCl, pH=8.0 , and then reacted with 500nM probe 2 in 1 / 15MPB, 0.3M NaCl, pH=7.4 solution for 2h at 25°C. Then wash the electrode three times with 1 / 15MPB, 0.1M NaCl, pH = 7.4 solution and store it at 4°C for future use.
Embodiment 3
[0041] Example 3: Construction of Hg when the whole thymine (T) oligonucleotide chain assembled on the surface of the gold electrode is at high density 2+ Detection of electrochemical sensors.
[0042] Soak an unmodified clean gold electrode in 100 mMPB containing 1 μM probe 1 that has been reduced with tris[2-carboxyethyl]phosphine (TCEP) for 1 h, 1.5 M NaCl, pH=7.2, and place it at room temperature overnight Assemble. The assembled electrode was rinsed twice with 10mM Tris, 0.5M NaCl, pH=8.0, then placed in an aqueous solution containing 1mM mercaptohexanol (MCH) to block for 30 minutes, and then washed three times with 10mM Tris, 0.5M NaCl, pH=8.0 , and then reacted with 500nM probe 2 in 1 / 15MPB, 0.3M NaCl, pH=7.4 solution for 2h at 25°C. Then wash the electrode three times with 1 / 15MPB, 0.1M NaCl, pH = 7.4 solution and store it at 4°C for future use.
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