Preparation method of three-dimensional soft bracket
A soft, three-dimensional technology, applied in the field of bioengineering, can solve problems such as difficulty in meeting the needs of organ soft scaffolds, inability to prepare organ soft scaffolds, etc., and achieve the effects of quality assurance, reduction of pollution probability, and efficiency improvement.
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[0023] In this example, primary liver cells are used to prepare liver tissue scaffolds to describe the preparation method of three-dimensional soft scaffolds, which specifically includes the following steps:
[0024] Step 1. Carry out CT or MRI scanning on the replaced liver, and obtain a set of tomographic images of the organ and tissue parts with a total of 30 layers from bottom to top. i , where the size of Δh is the diameter of the nozzle hole, here we take the diameter of the nozzle hole as 0.3mm, that is, Δh=0.3mm;
[0025] Step 2. Take 1.0×10 6 Primary hepatocytes from a patient were cultured. The cell culture medium was high-glucose DMEM medium, and 10ml of fetal bovine serum, 29.2mg of glutamine and 10mg of penicillin / streptomycin were added to each 100ml of culture medium. Reach the required quantity, digest with trypsin before use, then extract the obtained hepatocytes, and finally add DMEM cell culture medium to prepare a...
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