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Use of polyhexamethylene guanidine as animal specimen mildew preventive

A technology of polyhexamethylene guanidine and antifungal agent, applied in the field of antifungal agent for animal specimens, to achieve the effect of broad spectrum sterilization, fast action speed and low effective concentration

Inactive Publication Date: 2013-06-12
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polyhexamethylene guanidine has not been reported as an antifungal agent for animal specimens.

Method used

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  • Use of polyhexamethylene guanidine as animal specimen mildew preventive
  • Use of polyhexamethylene guanidine as animal specimen mildew preventive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Adopt the method of the present invention to carry out polyhexamethylene guanidine inhibition deer specimen mold experiment according to the following steps.

[0024] 1. Inoculation activation

[0025] First collect the mold on the surface of the animal specimen, culture it with PDA at 30°C, and then inoculate and activate the mold first;

[0026] Wash the mold spores on the slant of the test tube with sterilized water, use a pipette gun to draw 200mL of the bacterial suspension and add it to the PDA plate medium, and spread the bacterial suspension evenly with the coating rod (the coating rod should be washed with 95% ethanol or acetaldehyde beforehand). Burn and sterilize on an alcohol lamp), after standing for 5 minutes, place the plate at 30°C and incubate for 24-48 hours in a biochemical incubator, and it can be used as a seedling after it grows without bacteria.

[0027] 2. Inhibition experiment part

[0028] (1) Polyhexamethylene guanidine inhibits mold ...

Embodiment 2

[0034] Adopt the inventive method to carry out polyhexamethylene guanidine inhibition fish specimen mold experiment according to the following steps:

[0035] 1. Inoculation activation

[0036] First collect the mold on the surface of the animal specimen, culture it with PDA at 30°C, and then inoculate and activate the mold first;

[0037] Wash the mold spores on the slant of the test tube with sterilized water, use a pipette gun to draw 200mL of the bacterial suspension and add it to the PDA plate medium, and spread the bacterial suspension evenly with the coating rod (the coating rod should be washed with 95% ethanol or acetaldehyde beforehand). Burn and sterilize on an alcohol lamp), after standing for 5 minutes, place the plate in a biochemical incubator at 30°C for 24-48 hours. It can be used as a seedling after growing out without miscellaneous bacteria.

[0038] 2. Inhibition experiment part

[0039] (1) Polyhexamethylene guanidine inhibits mold in animal specim...

Embodiment 3

[0047] Applying polyhexamethylene guanidine solution with a concentration of 100 mol / liter to animal specimens can prevent mildew; for moldy animal specimens, smear 10cm on the surface of moldy body 2 The 100 mol / L polyhexamethylene guanidine solution was observed and photographed every week, and it was found that the mold was gradually inhibited in 15-30 days, and the mold gradually disappeared in 30-40 days.

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Abstract

The invention discloses use of polyhexamethylene guanidine as an animal specimen mildew preventive, and belongs to the technical field of specimen mildew preventives. A mildew preventive storage liquid is a solution with the total molar concentration of polyhexamethylene guanidine of 0.010-100.0 mol / L. The application method comprises the following step of: coating / soaking / or spraying a specimen by use of the solution to prevent mildew for the specimen. The polyhexamethylene guanidine is a completely safe / environmental-friendly specimen mildew preventing and treating method. The mildew preventive storage liquid is the mildew preventive consisting of polyhexamethylene guanidine, has broad spectrum in sterilization, and has excellent performances of low effective concentration, fast acting effect, stable property and easy solubility in water. Compared with current animal specimen mildew preventives marketed, the animal specimen mildew preventive has good antibacterial effect.

Description

technical field [0001] The invention relates to an antifungal agent for animal specimens, in particular to the use of polyhexamethyleneguanidine as an antifungal agent for animal specimens, and belongs to the technical field of antimold for animal specimens. Background technique [0002] There are many kinds of animals, and the methods of making and preserving specimens of different kinds of animals are also different. According to the production methods, animal specimens can be divided into stripped specimens, soaked specimens, bone specimens, dried specimens and embedded specimens, etc. The finished animal specimens can theoretically be preserved permanently. However, limited by various subjective and objective conditions, animal specimens are often damaged by various harmful factors during the preservation process, resulting in a greatly shortened service life of the specimens. [0003] Among the many factors that damage animal specimens, the harm of mold is the most ser...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/00
Inventor 张锐孙美榕
Owner GUANGDONG OCEAN UNIVERSITY