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Method for preparing collagen/lactic acid-caprolactone copolymer composite fiber bracket

A collagen and composite fiber technology, applied in the fields of medical science, textiles and papermaking, prostheses, etc., can solve the problem of not fully meeting the requirements of cell growth, and achieve the effect of promoting cell growth.

Active Publication Date: 2013-06-12
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The dense fibrous membrane obtained by stacking nanofibers layer by layer cannot fully meet the requirements of cell growth because its pore size is nanoscale, such as cells cannot grow into its interior.

Method used

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  • Method for preparing collagen/lactic acid-caprolactone copolymer composite fiber bracket
  • Method for preparing collagen/lactic acid-caprolactone copolymer composite fiber bracket
  • Method for preparing collagen/lactic acid-caprolactone copolymer composite fiber bracket

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Dissolve 0.8 g of collagen in 10 ml of hexafluoroisopropanol and stir until uniform to obtain a collagen solution with a concentration of 8% (g / ml); dissolve 0.8 g of polylactic acid-caprolactone copolymer in 10 ml In hexafluoroisopropanol, stir until uniform to obtain a lactic acid-caprolactone copolymer solution with a concentration of 8% (g / ml); mix the two solutions at a volume ratio of 10:90 to obtain collagen and lactic acid- A mixed solution with a caprolactone copolymer mass ratio of 10:90; the mixed solution was subjected to electrospinning, spinning conditions: voltage, 15 kV; electric field distance, 150 mm; injection rate, 1 ml / hour; shaft speed, 60 rotation / minute; the diameter of the small hole is 8 mm, and the composite fiber scaffold is obtained. The nanofibers in the scaffold have an average diameter of 650 nanometers, and the microfibers have an average diameter of 24 micrometers.

Embodiment 2

[0024] Dissolve 0.8 g of collagen in 10 ml of hexafluoroisopropanol and stir until uniform to obtain a collagen solution with a concentration of 8% (g / ml); dissolve 0.8 g of lactic acid-caprolactone copolymer in 10 ml of hexafluoroisopropanol In fluoroisopropanol, stir until uniform to obtain a polylactic acid-polycaprolactone solution with a concentration of 8% (g / ml); mix the two solutions at a volume ratio of 25:75 to obtain collagen and lactic acid-caprolactone A mixed solution with a lactone copolymer mass ratio of 25:75; electrospinning the mixed solution, spinning conditions: voltage, 15 kV; electric field distance, 150 mm; injection rate, 1 ml / hour; shaft speed, 60 rpm / min; the diameter of the small hole is 8 mm, and the composite fiber support is obtained. The nanofibers in the scaffold have an average diameter of 542 nanometers, and the microfibers have an average diameter of 20 micrometers.

Embodiment 3

[0026] Dissolve 0.8 g of collagen in 10 ml of hexafluoroisopropanol solution and stir until uniform to obtain a collagen solution with a concentration of 8% (g / ml); dissolve 0.8 g of lactic acid-caprolactone copolymer in 10 ml Hexafluoroisopropanol solution, stirred until uniform to obtain a lactic acid-caprolactone copolymer solution with a concentration of 8% (g / ml); mix the two solutions at a volume ratio of 40:60 to obtain collagen and poly A mixed solution with a mass ratio of lactic acid-polycaprolactone of 40:60; electrospinning the mixed solution, spinning conditions: voltage, 15 kV; electric field distance, 150 mm; injection rate, 1 ml / hour; shaft speed, 60 revolutions / minute; the diameter of the small hole is 8 mm, and the composite fiber scaffold is obtained. The nanofibers in the scaffold have an average diameter of 450 nanometers, and the microfibers have an average diameter of 15 micrometers.

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Abstract

The invention relates to a method for preparing a collagen / lactic acid-caprolactone copolymer composite fiber bracket. The method comprises the following steps of: (1) dissolving collagen into hexafluoroisopropanol, uniformly stirring to obtain a collagen solution with the concentration of 4 to 10 percent (g / ml), dissolving lactic acid-caprolactone copolymer into the hexafluoroisopropanol, and uniformly stirring to obtain a lactic acid-caprolactone copolymer solution with the concentration of 4 to 10 percent (g / ml); (2) mixing the collagen solution with the lactic acid-caprolactone copolymer solution to obtain a blended electrostatic spinning solution, wherein the volume ratio of the total solute to a solvent is 4 percent to 10 percent; and (3) performing electrostatic spinning on the blended electrostatic spinning solution to obtain the collagen / lactic acid-caprolactone copolymer composite fiber bracket. The aperture of the prepared bracket is increased along with the increase of fiber diameter. The bracket has the structural characteristics of nano- and micro-fibers, simulates an extracellular matrix, and has a great application prospect.

Description

technical field [0001] The invention belongs to the field of preparation of composite fiber scaffolds, in particular to a preparation method of collagen / lactic acid-caprolactone copolymer composite fiber scaffolds. Background technique [0002] The core of tissue engineering is to construct a three-dimensional space complex composed of biomaterials and cells, that is, tissue engineering scaffold composite seed cells. Its biggest advantage is that it can form living tissues with vitality, bionic human tissues and organs, reconstruct the shape, structure and function of damaged tissues and achieve permanent replacement; use less tissue cells to complete the repair and repair of damaged tissues. regeneration. In this process, tissue engineering scaffolds provide an appropriate microenvironment for cell growth and migration, including adhesion sites and growth signals. [0003] Tissue engineering scaffold is one of the key factors to determine the success of tissue repair. An...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): D04H1/4382D04H1/728A61L27/24A61L27/18A61L27/56
Inventor 莫秀梅吴晶磊
Owner DONGHUA UNIV
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