Blood coagulation quality control product and preparing method thereof
A coagulation quality control and coagulation technology, which is applied in the field of quality control of clinical coagulation test items, can solve the problems of high price, and achieve the effect of low raw material cost, stable coagulation factors, and easy access
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Embodiment 1
[0034] Example 1 Preparation of coagulation quality control products by adding fibrinogen solution
[0035] 1. Preparation of coagulation quality control products
[0036] 1. Rabbit and bovine blood were collected from the carotid artery, and pig blood was collected from the anterior vena cava. Add 3.8% trisodium citrate anticoagulant to the collected blood at a ratio of 9:1 to anticoagulant. The blood added with anticoagulant was centrifuged for 30 minutes with a relative centrifugal force of 2500g, and the upper layer of plasma was carefully separated and drawn.
[0037] 2. Preparation of plasma buffer containing stabilizer: Tris 20mM / L, sodium azide 1g / L, sucrose 20g / L, aprotinin 80000KIU / L, hydrochloric acid to adjust the pH value to 7.5.
[0038] 3. Detect the PT and APTT values of pig, cow and rabbit plasma, as shown in Table 1.
[0039] Table 1 Animal plasma PT and APTT values (unit: second)
[0040]
[0041] 4. Mix 74ml of pig plasma, 10ml of bovine plasma, ...
Embodiment 2
[0069] Example 2 Preparation of coagulation quality control products by removing part of fibrinogen
[0070] 1. Preparation of coagulation quality control products
[0071] 1. Rabbit and bovine blood were collected from the carotid artery, and pig blood was collected from the anterior vena cava. Add 3.8% trisodium citrate anticoagulant to the collected blood at a ratio of 9:1 to the anticoagulant, centrifuge for 30 minutes at a relative centrifugal force of 2500g, and carefully separate and absorb the upper layer of plasma.
[0072] 2. Preparation of plasma buffer containing stabilizer: Tris 20mM / L, sodium azide 1g / L, sucrose 20g / L, aprotinin 80000KIU / L, hydrochloric acid to adjust the pH value to 7.5.
[0073] 3. Detect the PT and APTT values of pig, cow and rabbit plasma, as shown in Table 8.
[0074] Table 8 Animal plasma PT and APTT values (unit: second)
[0075]
[0076] 4. Mix 146ml of pig plasma, 34ml of bovine plasma, and 20ml of rabbit plasma, and test the P...
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