Application of fatty acid compounds in the preparation of drugs for the prevention and treatment of liver cancer
A fatty acid treatment technology for liver cancer, applied in drug combinations, digestive system, anti-tumor drugs, etc., can solve problems such as inability to perform normal functions, and achieve the effect of enhancing activity inhibition and promoting apoptosis
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Embodiment 1
[0038] The preparation of embodiment 1 fatty acid compound
[0039] 1. Prepare the alcoholic extract of Angelica dahurica
[0040] 1) Weigh 3kg of dried Angelica dahurica root, add 30L of 60% ethanol, stir, boil and reflux for 2 hours in the extraction tank, filter, and collect the filtrate;
[0041] 2) The filter residue was boiled and refluxed with 60% ethanol to extract twice, filtered, and the filtrate was collected. The dosage of 60% ethanol was 30L / time;
[0042] 3) Merge the filtrates for 3 times, extract with ethyl acetate, the amount of extractant is 30L each time, and collect the upper layer extract;
[0043] 4) The lower layer was extracted twice with ethyl acetate, and the upper layer extracts were combined for three times, and vacuum-dried to obtain 48.3g of the alcoholic extract of Angelica dahurica (coded as: AD).
[0044] 2. Silica gel column chromatography and ODS column chromatography to prepare monomeric compounds
[0045] 1) Soak 200-300 mesh silica gel ...
Embodiment 2
[0058] Embodiment 2 fatty acid compound pharmacodynamics experiment
[0059] 1. Experimental materials and reagents
[0060] (1) Experimental cell: human liver cancer cell HepG2
[0061] (2) Strains
[0062] pGEX4T1-PTP-SHP2 E. coli BL21
[0063] pGEX4T1-VHR E. coli BL21
[0064] pGEX4T1-HEPTP E. coli BL21
[0065] (3) Medicinal material Hangzhou Angelica dahurica produced in Zhejiang
[0066] (4) Main reagents
[0067]
[0068] (5) culture medium
[0069] LB liquid medium:
[0070] 1% (g / ml) Tryptone, 0.5% (g / ml) Yeast Extract, 1% (g / ml) NaCl
[0071] (6) Buffers and reagents required for GST protein purification
[0072] PBS Bμffer: 137mM NaCl, 2.7mM KCl, 10mM NaCl 2 HPO 4 ,2mM KH 2 PO 4
[0073] 100mg / ml ampicillin
[0074] Protease Inhibitor and Phosphatase Inhibitor Stock Solutions:
[0075]
[0076]
[0077] IPTG stock solution (0.8M)
[0078] Buffer W: 25mM Tris-HCl pH7.5, 150mM NaCl, 10mM β-mercaptoethanol, 1mM EDTA
[0079] Buffer W1: 25mM ...
Embodiment 314
[0197] Embodiment 314Z, the preparation of 17Z-eicosadienoic acid tablet
[0198] Get 14Z, 17Z-eicosadienoic acid 1g, microcrystalline cellulose 27g and magnesium stearate 2g and mix, and the mixture is pressed into a tablet with a diameter of 6mm and a weight of 300mg with a single-punch tablet machine. The tablet contains 10mg of 14Z, 17Z-21-eicosadienoic acid.
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